Experiment: RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05960 and BWI76_RS05965 overlap by 75 nucleotides BWI76_RS05965 and BWI76_RS05970 are separated by 70 nucleotides BWI76_RS05970 and BWI76_RS05975 are separated by 107 nucleotides BWI76_RS05975 and BWI76_RS05980 overlap by 8 nucleotides BWI76_RS05980 and BWI76_RS05985 overlap by 14 nucleotides
BWI76_RS05960: BWI76_RS05960 - cysteine transport system periplasmic binding protein, at 1,252,622 to 1,253,431
_RS05960
BWI76_RS05965: BWI76_RS05965 - hypothetical protein, at 1,253,357 to 1,253,674
_RS05965
BWI76_RS05970: BWI76_RS05970 - hypothetical protein, at 1,253,745 to 1,253,927
_RS05970
BWI76_RS05975: BWI76_RS05975 - ABC transporter ATP-binding protein, at 1,254,035 to 1,254,730
_RS05975
BWI76_RS05980: BWI76_RS05980 - ABC transporter ATP-binding protein, at 1,254,723 to 1,255,505
_RS05980
BWI76_RS05985: BWI76_RS05985 - branched-chain amino acid ABC transporter permease, at 1,255,492 to 1,256,541
_RS05985
Position (kb)
1254
1255 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1253.154 kb on - strand, within BWI76_RS05960 at 1253.193 kb on + strand, within BWI76_RS05960 at 1253.211 kb on - strand, within BWI76_RS05960 at 1253.254 kb on - strand, within BWI76_RS05960 at 1253.284 kb on + strand, within BWI76_RS05960 at 1253.364 kb on + strand at 1253.364 kb on + strand at 1253.372 kb on - strand at 1253.372 kb on - strand at 1253.372 kb on - strand at 1253.372 kb on - strand at 1253.604 kb on - strand, within BWI76_RS05965 at 1253.606 kb on - strand, within BWI76_RS05965 at 1253.699 kb on + strand at 1253.707 kb on - strand at 1253.798 kb on + strand, within BWI76_RS05970 at 1253.880 kb on - strand, within BWI76_RS05970 at 1254.022 kb on + strand at 1254.030 kb on - strand at 1254.030 kb on - strand at 1254.030 kb on - strand at 1254.062 kb on - strand at 1254.255 kb on - strand, within BWI76_RS05975 at 1254.403 kb on + strand, within BWI76_RS05975 at 1254.411 kb on - strand, within BWI76_RS05975 at 1254.432 kb on + strand, within BWI76_RS05975 at 1254.675 kb on + strand at 1254.794 kb on - strand at 1254.816 kb on - strand, within BWI76_RS05980 at 1254.856 kb on + strand, within BWI76_RS05980 at 1255.055 kb on - strand, within BWI76_RS05980 at 1255.099 kb on + strand, within BWI76_RS05980 at 1255.099 kb on + strand, within BWI76_RS05980 at 1255.182 kb on + strand, within BWI76_RS05980 at 1255.338 kb on - strand, within BWI76_RS05980 at 1255.352 kb on + strand at 1255.384 kb on + strand, within BWI76_RS05980 at 1255.465 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 1,253,154 - BWI76_RS05960 0.66 -0.7 1,253,193 + BWI76_RS05960 0.70 +0.4 1,253,211 - BWI76_RS05960 0.73 +0.1 1,253,254 - BWI76_RS05960 0.78 -3.5 1,253,284 + BWI76_RS05960 0.82 -1.1 1,253,364 + +0.2 1,253,364 + +0.1 1,253,372 - -0.2 1,253,372 - +0.2 1,253,372 - +0.5 1,253,372 - +0.9 1,253,604 - BWI76_RS05965 0.78 -1.5 1,253,606 - BWI76_RS05965 0.78 -1.1 1,253,699 + +0.2 1,253,707 - +0.9 1,253,798 + BWI76_RS05970 0.29 -2.8 1,253,880 - BWI76_RS05970 0.74 +1.1 1,254,022 + +2.0 1,254,030 - +0.5 1,254,030 - -0.5 1,254,030 - +1.2 1,254,062 - +0.1 1,254,255 - BWI76_RS05975 0.32 -0.7 1,254,403 + BWI76_RS05975 0.53 +1.1 1,254,411 - BWI76_RS05975 0.54 +0.9 1,254,432 + BWI76_RS05975 0.57 +1.2 1,254,675 + +0.6 1,254,794 - -0.6 1,254,816 - BWI76_RS05980 0.12 +0.2 1,254,856 + BWI76_RS05980 0.17 -0.1 1,255,055 - BWI76_RS05980 0.42 -0.1 1,255,099 + BWI76_RS05980 0.48 -0.1 1,255,099 + BWI76_RS05980 0.48 -1.2 1,255,182 + BWI76_RS05980 0.59 +0.5 1,255,338 - BWI76_RS05980 0.79 +0.2 1,255,352 + +0.9 1,255,384 + BWI76_RS05980 0.84 -0.2 1,255,465 + +1.3
Or see this region's nucleotide sequence