Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS05625

Experiment: RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS05620 and BWI76_RS05625 are separated by 252 nucleotidesBWI76_RS05625 and BWI76_RS05630 are separated by 40 nucleotides BWI76_RS05620: BWI76_RS05620 - NAD-dependent succinate-semialdehyde dehydrogenase, at 1,178,560 to 1,180,008 _RS05620 BWI76_RS05625: BWI76_RS05625 - Zn-finger protein, at 1,180,261 to 1,180,512 _RS05625 BWI76_RS05630: BWI76_RS05630 - TonB-dependent siderophore receptor, at 1,180,553 to 1,182,643 _RS05630 Position (kb) 1180 1181Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 1179.403 kb on + strand, within BWI76_RS05620at 1179.467 kb on + strand, within BWI76_RS05620at 1179.783 kb on + strand, within BWI76_RS05620at 1179.859 kb on + strand, within BWI76_RS05620at 1180.028 kb on - strandat 1180.062 kb on + strandat 1180.371 kb on + strand, within BWI76_RS05625at 1180.659 kb on - strandat 1180.702 kb on + strandat 1180.710 kb on - strandat 1180.729 kb on + strandat 1180.851 kb on + strand, within BWI76_RS05630at 1180.941 kb on - strand, within BWI76_RS05630at 1181.060 kb on + strand, within BWI76_RS05630at 1181.085 kb on + strand, within BWI76_RS05630at 1181.338 kb on - strand, within BWI76_RS05630

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
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1,179,403 + BWI76_RS05620 0.58 -0.6
1,179,467 + BWI76_RS05620 0.63 +0.4
1,179,783 + BWI76_RS05620 0.84 -0.4
1,179,859 + BWI76_RS05620 0.90 +4.2
1,180,028 - -0.5
1,180,062 + -1.4
1,180,371 + BWI76_RS05625 0.44 -1.5
1,180,659 - -0.3
1,180,702 + -0.1
1,180,710 - +0.8
1,180,729 + -0.6
1,180,851 + BWI76_RS05630 0.14 +0.2
1,180,941 - BWI76_RS05630 0.19 +0.7
1,181,060 + BWI76_RS05630 0.24 +2.5
1,181,085 + BWI76_RS05630 0.25 -0.1
1,181,338 - BWI76_RS05630 0.38 -1.9

Or see this region's nucleotide sequence