Experiment: RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05440 and BWI76_RS05445 are separated by 32 nucleotides BWI76_RS05445 and BWI76_RS05450 overlap by 4 nucleotides BWI76_RS05450 and BWI76_RS05455 are separated by 65 nucleotides BWI76_RS05455 and BWI76_RS05460 are separated by 132 nucleotides
BWI76_RS05440: BWI76_RS05440 - hydroxyacylglutathione hydrolase, at 1,142,455 to 1,143,210
_RS05440
BWI76_RS05445: BWI76_RS05445 - type 11 methyltransferase, at 1,143,243 to 1,143,965
_RS05445
BWI76_RS05450: BWI76_RS05450 - ribonuclease HI, at 1,143,962 to 1,144,429
_RS05450
BWI76_RS05455: BWI76_RS05455 - DNA polymerase III subunit epsilon, at 1,144,495 to 1,145,226
_RS05455
BWI76_RS05460: BWI76_RS05460 - tRNA-Asp, at 1,145,359 to 1,145,435
_RS05460
Position (kb)
1143
1144
1145 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1143.029 kb on + strand, within BWI76_RS05440 at 1143.076 kb on - strand, within BWI76_RS05440 at 1143.264 kb on - strand at 1143.294 kb on + strand at 1143.352 kb on - strand, within BWI76_RS05445 at 1143.352 kb on - strand, within BWI76_RS05445 at 1143.470 kb on + strand, within BWI76_RS05445 at 1143.470 kb on + strand, within BWI76_RS05445 at 1143.478 kb on - strand, within BWI76_RS05445 at 1143.478 kb on - strand, within BWI76_RS05445 at 1143.478 kb on - strand, within BWI76_RS05445 at 1143.478 kb on - strand, within BWI76_RS05445 at 1143.622 kb on + strand, within BWI76_RS05445 at 1143.641 kb on + strand, within BWI76_RS05445 at 1144.210 kb on + strand, within BWI76_RS05450 at 1144.317 kb on - strand, within BWI76_RS05450 at 1144.492 kb on + strand at 1145.308 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 1,143,029 + BWI76_RS05440 0.76 +0.3 1,143,076 - BWI76_RS05440 0.82 +1.4 1,143,264 - +1.0 1,143,294 + +0.8 1,143,352 - BWI76_RS05445 0.15 +0.3 1,143,352 - BWI76_RS05445 0.15 +0.8 1,143,470 + BWI76_RS05445 0.31 +1.0 1,143,470 + BWI76_RS05445 0.31 -0.1 1,143,478 - BWI76_RS05445 0.33 +1.2 1,143,478 - BWI76_RS05445 0.33 +1.0 1,143,478 - BWI76_RS05445 0.33 +2.5 1,143,478 - BWI76_RS05445 0.33 -2.4 1,143,622 + BWI76_RS05445 0.52 +3.0 1,143,641 + BWI76_RS05445 0.55 -1.4 1,144,210 + BWI76_RS05450 0.53 -1.9 1,144,317 - BWI76_RS05450 0.76 +0.9 1,144,492 + -0.4 1,145,308 + +1.4
Or see this region's nucleotide sequence