Experiment: RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05035 and BWI76_RS05040 overlap by 1 nucleotides BWI76_RS05040 and BWI76_RS05045 overlap by 11 nucleotides BWI76_RS05045 and BWI76_RS05050 overlap by 8 nucleotides
BWI76_RS05035: BWI76_RS05035 - prepilin peptidase, at 1,056,453 to 1,057,283
_RS05035
BWI76_RS05040: BWI76_RS05040 - type II secretion system protein N, at 1,057,283 to 1,058,041
_RS05040
BWI76_RS05045: BWI76_RS05045 - general secretion pathway protein GspM, at 1,058,031 to 1,058,516
_RS05045
BWI76_RS05050: BWI76_RS05050 - general secretion pathway protein GspL, at 1,058,509 to 1,059,705
_RS05050
Position (kb)
1057
1058
1059 Strain fitness (log2 ratio)
-1
0
1
2 at 1056.526 kb on + strand at 1056.597 kb on - strand, within BWI76_RS05035 at 1057.096 kb on - strand at 1057.373 kb on + strand, within BWI76_RS05040 at 1057.468 kb on + strand, within BWI76_RS05040 at 1057.486 kb on - strand, within BWI76_RS05040 at 1057.520 kb on + strand, within BWI76_RS05040 at 1057.946 kb on - strand, within BWI76_RS05040 at 1058.457 kb on - strand, within BWI76_RS05045 at 1058.457 kb on - strand, within BWI76_RS05045 at 1058.541 kb on + strand at 1058.616 kb on + strand at 1058.624 kb on - strand at 1058.624 kb on - strand at 1059.018 kb on + strand, within BWI76_RS05050
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 1,056,526 + +0.1 1,056,597 - BWI76_RS05035 0.17 +1.1 1,057,096 - +0.9 1,057,373 + BWI76_RS05040 0.12 -0.6 1,057,468 + BWI76_RS05040 0.24 -0.7 1,057,486 - BWI76_RS05040 0.27 -0.8 1,057,520 + BWI76_RS05040 0.31 -1.1 1,057,946 - BWI76_RS05040 0.87 -0.7 1,058,457 - BWI76_RS05045 0.88 -1.5 1,058,457 - BWI76_RS05045 0.88 +0.1 1,058,541 + -0.9 1,058,616 + -0.9 1,058,624 - +0.4 1,058,624 - +1.9 1,059,018 + BWI76_RS05050 0.43 -0.6
Or see this region's nucleotide sequence