Experiment: RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04165 and BWI76_RS04170 are separated by 21 nucleotides BWI76_RS04170 and BWI76_RS04175 are separated by 98 nucleotides
BWI76_RS04165: BWI76_RS04165 - DNA repair protein RadA, at 856,653 to 858,035
_RS04165
BWI76_RS04170: BWI76_RS04170 - trifunctional nicotinamide-nucleotide adenylyltransferase/ribosylnicotinamide kinase/transcriptional regulator NadR, at 858,057 to 859,289
_RS04170
BWI76_RS04175: BWI76_RS04175 - energy-dependent translational throttle protein EttA, at 859,388 to 861,055
_RS04175
Position (kb) 858
859
860 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 857.087 kb on - strand, within BWI76_RS04165 at 857.376 kb on - strand, within BWI76_RS04165 at 857.473 kb on + strand, within BWI76_RS04165 at 857.652 kb on + strand, within BWI76_RS04165 at 857.682 kb on + strand, within BWI76_RS04165 at 857.999 kb on + strand at 858.067 kb on - strand at 858.314 kb on + strand, within BWI76_RS04170 at 858.324 kb on - strand, within BWI76_RS04170 at 858.530 kb on - strand, within BWI76_RS04170 at 858.594 kb on - strand, within BWI76_RS04170 at 858.772 kb on - strand, within BWI76_RS04170 at 858.893 kb on + strand, within BWI76_RS04170 at 859.069 kb on + strand, within BWI76_RS04170 at 859.127 kb on - strand, within BWI76_RS04170 at 859.167 kb on + strand at 859.208 kb on - strand at 859.257 kb on - strand at 859.318 kb on + strand at 859.507 kb on + strand at 859.589 kb on + strand, within BWI76_RS04175 at 859.713 kb on + strand, within BWI76_RS04175 at 859.742 kb on - strand, within BWI76_RS04175 at 860.196 kb on + strand, within BWI76_RS04175
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 857,087 - BWI76_RS04165 0.31 -0.4 857,376 - BWI76_RS04165 0.52 +0.9 857,473 + BWI76_RS04165 0.59 -1.3 857,652 + BWI76_RS04165 0.72 -0.8 857,682 + BWI76_RS04165 0.74 +1.2 857,999 + +0.0 858,067 - -0.3 858,314 + BWI76_RS04170 0.21 -0.2 858,324 - BWI76_RS04170 0.22 +0.5 858,530 - BWI76_RS04170 0.38 -1.1 858,594 - BWI76_RS04170 0.44 +0.2 858,772 - BWI76_RS04170 0.58 +0.6 858,893 + BWI76_RS04170 0.68 +0.0 859,069 + BWI76_RS04170 0.82 +1.0 859,127 - BWI76_RS04170 0.87 -4.5 859,167 + +2.5 859,208 - +0.6 859,257 - +1.0 859,318 + +3.6 859,507 + +0.6 859,589 + BWI76_RS04175 0.12 +1.0 859,713 + BWI76_RS04175 0.19 +0.6 859,742 - BWI76_RS04175 0.21 -0.5 860,196 + BWI76_RS04175 0.48 -0.8
Or see this region's nucleotide sequence