Experiment: RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00885 and BWI76_RS00890 are separated by 364 nucleotides BWI76_RS00890 and BWI76_RS00895 overlap by 56 nucleotides BWI76_RS00895 and BWI76_RS00900 overlap by 20 nucleotides
BWI76_RS00885: BWI76_RS00885 - tRNA (uridine(54)-C5)-methyltransferase TrmA, at 181,984 to 183,084
_RS00885
BWI76_RS00890: BWI76_RS00890 - vitamin B12/cobalamin outer membrane transporter, at 183,449 to 185,317
_RS00890
BWI76_RS00895: BWI76_RS00895 - glutamate racemase, at 185,262 to 186,116
_RS00895
BWI76_RS00900: BWI76_RS00900 - hypothetical protein, at 186,097 to 186,364
_RS00900
Position (kb) 183
184
185
186 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 182.575 kb on - strand, within BWI76_RS00885 at 182.637 kb on + strand, within BWI76_RS00885 at 182.713 kb on + strand, within BWI76_RS00885 at 182.772 kb on + strand, within BWI76_RS00885 at 182.812 kb on - strand, within BWI76_RS00885 at 183.052 kb on + strand at 183.062 kb on + strand at 183.078 kb on - strand at 183.079 kb on + strand at 183.121 kb on - strand at 183.129 kb on + strand at 183.140 kb on + strand at 183.140 kb on + strand at 183.140 kb on + strand at 183.145 kb on + strand at 183.231 kb on + strand at 183.236 kb on + strand at 183.290 kb on - strand at 183.328 kb on + strand at 183.354 kb on - strand at 183.360 kb on - strand at 183.413 kb on - strand at 183.419 kb on - strand at 183.428 kb on + strand at 183.527 kb on - strand at 183.535 kb on + strand at 183.536 kb on - strand at 183.567 kb on + strand at 183.613 kb on - strand at 183.753 kb on - strand, within BWI76_RS00890 at 183.758 kb on + strand, within BWI76_RS00890 at 183.786 kb on + strand, within BWI76_RS00890 at 183.830 kb on + strand, within BWI76_RS00890 at 183.872 kb on + strand, within BWI76_RS00890 at 183.880 kb on - strand, within BWI76_RS00890 at 183.894 kb on - strand, within BWI76_RS00890 at 183.942 kb on + strand, within BWI76_RS00890 at 183.942 kb on + strand, within BWI76_RS00890 at 183.942 kb on + strand, within BWI76_RS00890 at 183.945 kb on - strand, within BWI76_RS00890 at 183.951 kb on - strand, within BWI76_RS00890 at 184.005 kb on - strand, within BWI76_RS00890 at 184.017 kb on + strand, within BWI76_RS00890 at 184.017 kb on + strand, within BWI76_RS00890 at 184.066 kb on + strand, within BWI76_RS00890 at 184.121 kb on - strand, within BWI76_RS00890 at 184.170 kb on - strand, within BWI76_RS00890 at 184.177 kb on - strand, within BWI76_RS00890 at 184.189 kb on - strand, within BWI76_RS00890 at 184.282 kb on + strand, within BWI76_RS00890 at 184.290 kb on + strand, within BWI76_RS00890 at 184.297 kb on + strand, within BWI76_RS00890 at 184.334 kb on - strand, within BWI76_RS00890 at 184.390 kb on + strand, within BWI76_RS00890 at 184.445 kb on + strand, within BWI76_RS00890 at 184.448 kb on - strand, within BWI76_RS00890 at 184.448 kb on - strand, within BWI76_RS00890 at 184.625 kb on + strand, within BWI76_RS00890 at 184.680 kb on + strand, within BWI76_RS00890 at 184.708 kb on - strand, within BWI76_RS00890 at 184.779 kb on - strand, within BWI76_RS00890 at 184.855 kb on + strand, within BWI76_RS00890 at 184.855 kb on + strand, within BWI76_RS00890 at 184.860 kb on + strand, within BWI76_RS00890 at 184.938 kb on - strand, within BWI76_RS00890 at 184.990 kb on + strand, within BWI76_RS00890 at 185.061 kb on - strand, within BWI76_RS00890 at 185.095 kb on + strand, within BWI76_RS00890 at 185.156 kb on - strand at 185.169 kb on + strand at 185.258 kb on + strand at 186.174 kb on + strand, within BWI76_RS00900
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 182,575 - BWI76_RS00885 0.54 -2.0 182,637 + BWI76_RS00885 0.59 -2.0 182,713 + BWI76_RS00885 0.66 -0.6 182,772 + BWI76_RS00885 0.72 -0.9 182,812 - BWI76_RS00885 0.75 +0.2 183,052 + -0.8 183,062 + +0.2 183,078 - -0.4 183,079 + +1.2 183,121 - +1.2 183,129 + -2.0 183,140 + -0.7 183,140 + +0.2 183,140 + +1.6 183,145 + +0.0 183,231 + +1.0 183,236 + -3.2 183,290 - -2.4 183,328 + +0.1 183,354 - +0.2 183,360 - -0.1 183,413 - -2.1 183,419 - +1.4 183,428 + -1.4 183,527 - +1.0 183,535 + -0.1 183,536 - -1.2 183,567 + -0.5 183,613 - +1.2 183,753 - BWI76_RS00890 0.16 +0.4 183,758 + BWI76_RS00890 0.17 +0.1 183,786 + BWI76_RS00890 0.18 -0.4 183,830 + BWI76_RS00890 0.20 +0.5 183,872 + BWI76_RS00890 0.23 +0.8 183,880 - BWI76_RS00890 0.23 +0.1 183,894 - BWI76_RS00890 0.24 -1.4 183,942 + BWI76_RS00890 0.26 -0.1 183,942 + BWI76_RS00890 0.26 -0.6 183,942 + BWI76_RS00890 0.26 -1.1 183,945 - BWI76_RS00890 0.27 -1.8 183,951 - BWI76_RS00890 0.27 -1.1 184,005 - BWI76_RS00890 0.30 +0.2 184,017 + BWI76_RS00890 0.30 -1.1 184,017 + BWI76_RS00890 0.30 -0.1 184,066 + BWI76_RS00890 0.33 -1.4 184,121 - BWI76_RS00890 0.36 +1.2 184,170 - BWI76_RS00890 0.39 -0.3 184,177 - BWI76_RS00890 0.39 -1.4 184,189 - BWI76_RS00890 0.40 -0.4 184,282 + BWI76_RS00890 0.45 -1.0 184,290 + BWI76_RS00890 0.45 -1.6 184,297 + BWI76_RS00890 0.45 -1.5 184,334 - BWI76_RS00890 0.47 +0.1 184,390 + BWI76_RS00890 0.50 +0.5 184,445 + BWI76_RS00890 0.53 -2.1 184,448 - BWI76_RS00890 0.53 +0.3 184,448 - BWI76_RS00890 0.53 -0.1 184,625 + BWI76_RS00890 0.63 -1.6 184,680 + BWI76_RS00890 0.66 +1.6 184,708 - BWI76_RS00890 0.67 -0.2 184,779 - BWI76_RS00890 0.71 -0.8 184,855 + BWI76_RS00890 0.75 -0.2 184,855 + BWI76_RS00890 0.75 -0.2 184,860 + BWI76_RS00890 0.75 -2.3 184,938 - BWI76_RS00890 0.80 -0.2 184,990 + BWI76_RS00890 0.82 -0.8 185,061 - BWI76_RS00890 0.86 +0.3 185,095 + BWI76_RS00890 0.88 -0.7 185,156 - -1.1 185,169 + +0.3 185,258 + -2.4 186,174 + BWI76_RS00900 0.29 -1.1
Or see this region's nucleotide sequence