Experiment: BHIS with DAMPA 60 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0464 and BT0465 are separated by 3 nucleotides BT0465 and BT0466 are separated by 2 nucleotides BT0466 and BT0467 are separated by 202 nucleotides
BT0464: BT0464 - dTDP-4-dehydrorhamnose 3,5-epimerase (NCBI ptt file), at 574,814 to 575,383
BT0464
BT0465: BT0465 - dTDP-4-dehydrorhamnose reductase (NCBI ptt file), at 575,387 to 576,241
BT0465
BT0466: BT0466 - dTDP-glucose 4,6-dehydratase (NCBI ptt file), at 576,244 to 577,356
BT0466
BT0467: BT0467 - conserved hypothetical protein (NCBI ptt file), at 577,559 to 579,028
BT0467
Position (kb) 576
577
578 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 575.376 kb on + strand at 575.410 kb on + strand at 575.461 kb on + strand at 575.470 kb on - strand at 575.527 kb on - strand, within BT0465 at 575.547 kb on + strand, within BT0465 at 575.582 kb on - strand, within BT0465 at 575.624 kb on - strand, within BT0465 at 575.680 kb on + strand, within BT0465 at 575.710 kb on + strand, within BT0465 at 575.716 kb on + strand, within BT0465 at 575.744 kb on - strand, within BT0465 at 575.840 kb on + strand, within BT0465 at 575.894 kb on - strand, within BT0465 at 575.936 kb on - strand, within BT0465 at 576.005 kb on - strand, within BT0465 at 576.005 kb on - strand, within BT0465 at 576.005 kb on - strand, within BT0465 at 576.008 kb on + strand, within BT0465 at 576.148 kb on - strand, within BT0465 at 576.155 kb on - strand, within BT0465 at 576.340 kb on - strand at 576.409 kb on - strand, within BT0466 at 576.409 kb on - strand, within BT0466 at 576.409 kb on - strand, within BT0466 at 576.422 kb on + strand, within BT0466 at 576.475 kb on - strand, within BT0466 at 576.525 kb on + strand, within BT0466 at 576.652 kb on - strand, within BT0466 at 576.754 kb on - strand, within BT0466 at 576.754 kb on - strand, within BT0466 at 576.789 kb on + strand, within BT0466 at 576.891 kb on + strand, within BT0466 at 576.974 kb on - strand, within BT0466 at 576.974 kb on - strand, within BT0466 at 576.978 kb on - strand, within BT0466 at 577.039 kb on - strand, within BT0466 at 577.042 kb on - strand, within BT0466 at 577.110 kb on - strand, within BT0466 at 577.113 kb on - strand, within BT0466 at 577.147 kb on - strand, within BT0466 at 577.179 kb on - strand, within BT0466 at 577.278 kb on - strand at 577.278 kb on - strand at 577.343 kb on - strand at 577.442 kb on - strand at 577.494 kb on - strand at 577.517 kb on - strand at 577.537 kb on + strand at 577.578 kb on + strand at 577.579 kb on - strand at 577.676 kb on - strand at 577.678 kb on - strand at 577.774 kb on - strand, within BT0467 at 577.778 kb on - strand, within BT0467 at 577.825 kb on - strand, within BT0467 at 577.855 kb on + strand, within BT0467 at 577.943 kb on - strand, within BT0467 at 577.955 kb on - strand, within BT0467 at 577.955 kb on - strand, within BT0467 at 578.041 kb on - strand, within BT0467 at 578.112 kb on - strand, within BT0467
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with DAMPA 60 uM remove 575,376 + +1.3 575,410 + -0.7 575,461 + +0.8 575,470 - +1.1 575,527 - BT0465 0.16 -1.7 575,547 + BT0465 0.19 -2.1 575,582 - BT0465 0.23 -0.6 575,624 - BT0465 0.28 -2.4 575,680 + BT0465 0.34 -1.1 575,710 + BT0465 0.38 +0.3 575,716 + BT0465 0.38 -1.7 575,744 - BT0465 0.42 -1.3 575,840 + BT0465 0.53 -1.3 575,894 - BT0465 0.59 +0.8 575,936 - BT0465 0.64 -2.3 576,005 - BT0465 0.72 -1.3 576,005 - BT0465 0.72 -2.6 576,005 - BT0465 0.72 -2.6 576,008 + BT0465 0.73 -0.7 576,148 - BT0465 0.89 -1.3 576,155 - BT0465 0.90 -0.3 576,340 - -0.7 576,409 - BT0466 0.15 +0.3 576,409 - BT0466 0.15 -0.7 576,409 - BT0466 0.15 -1.7 576,422 + BT0466 0.16 -1.3 576,475 - BT0466 0.21 -1.7 576,525 + BT0466 0.25 -1.0 576,652 - BT0466 0.37 +0.3 576,754 - BT0466 0.46 -0.7 576,754 - BT0466 0.46 -2.7 576,789 + BT0466 0.49 -2.3 576,891 + BT0466 0.58 -2.9 576,974 - BT0466 0.66 +0.7 576,974 - BT0466 0.66 -0.7 576,978 - BT0466 0.66 +0.3 577,039 - BT0466 0.71 +2.3 577,042 - BT0466 0.72 -3.1 577,110 - BT0466 0.78 -2.6 577,113 - BT0466 0.78 -0.7 577,147 - BT0466 0.81 -2.1 577,179 - BT0466 0.84 -2.3 577,278 - -0.7 577,278 - -1.7 577,343 - -1.3 577,442 - -0.7 577,494 - -3.1 577,517 - +0.3 577,537 + +0.3 577,578 + +0.3 577,579 - +0.3 577,676 - -0.7 577,678 - +0.3 577,774 - BT0467 0.15 -1.6 577,778 - BT0467 0.15 +1.3 577,825 - BT0467 0.18 -0.2 577,855 + BT0467 0.20 -1.3 577,943 - BT0467 0.26 +0.3 577,955 - BT0467 0.27 -0.2 577,955 - BT0467 0.27 -2.3 578,041 - BT0467 0.33 -0.7 578,112 - BT0467 0.38 -1.7
Or see this region's nucleotide sequence