Experiment: BHIS with DAMPA 60 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0194 and BT0195 are separated by 20 nucleotides BT0195 and BT0196 are separated by 12 nucleotides BT0196 and BT0197 are separated by 258 nucleotides
BT0194: BT0194 - hypothetical protein (NCBI ptt file), at 207,864 to 208,922
BT0194
BT0195: BT0195 - glycerophosphoryl diester phosphodiesterase (NCBI ptt file), at 208,943 to 209,842
BT0195
BT0196: BT0196 - putative hexose phosphate transport protein (NCBI ptt file), at 209,855 to 211,201
BT0196
BT0197: BT0197 - hypothetical protein (NCBI ptt file), at 211,460 to 212,059
BT0197
Position (kb) 209
210
211
212 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 208.869 kb on + strand at 208.870 kb on - strand at 209.131 kb on + strand, within BT0195 at 209.279 kb on - strand, within BT0195 at 209.356 kb on - strand, within BT0195 at 209.356 kb on - strand, within BT0195 at 209.400 kb on - strand, within BT0195 at 209.491 kb on - strand, within BT0195 at 209.589 kb on - strand, within BT0195 at 209.589 kb on - strand, within BT0195 at 209.598 kb on + strand, within BT0195 at 209.644 kb on - strand, within BT0195 at 209.803 kb on - strand at 209.870 kb on - strand at 209.918 kb on - strand at 209.997 kb on - strand, within BT0196 at 209.997 kb on - strand, within BT0196 at 209.997 kb on - strand, within BT0196 at 210.000 kb on - strand, within BT0196 at 210.100 kb on + strand, within BT0196 at 210.104 kb on + strand, within BT0196 at 210.148 kb on + strand, within BT0196 at 210.335 kb on + strand, within BT0196 at 210.365 kb on - strand, within BT0196 at 210.385 kb on + strand, within BT0196 at 210.393 kb on + strand, within BT0196 at 210.401 kb on - strand, within BT0196 at 210.468 kb on + strand, within BT0196 at 210.475 kb on - strand, within BT0196 at 210.640 kb on + strand, within BT0196 at 210.641 kb on - strand, within BT0196 at 210.644 kb on + strand, within BT0196 at 210.656 kb on + strand, within BT0196 at 210.755 kb on - strand, within BT0196 at 210.758 kb on - strand, within BT0196 at 210.850 kb on - strand, within BT0196 at 210.856 kb on - strand, within BT0196 at 210.871 kb on + strand, within BT0196 at 210.973 kb on + strand, within BT0196 at 211.028 kb on + strand, within BT0196 at 211.102 kb on - strand at 211.122 kb on - strand at 211.375 kb on + strand at 211.416 kb on + strand at 211.526 kb on - strand, within BT0197 at 211.551 kb on + strand, within BT0197 at 211.584 kb on + strand, within BT0197 at 211.745 kb on + strand, within BT0197 at 211.842 kb on - strand, within BT0197 at 211.872 kb on - strand, within BT0197 at 211.872 kb on - strand, within BT0197 at 211.930 kb on + strand, within BT0197 at 211.956 kb on + strand, within BT0197 at 212.027 kb on - strand at 212.071 kb on + strand at 212.074 kb on + strand at 212.079 kb on + strand at 212.089 kb on + strand at 212.143 kb on - strand at 212.158 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with DAMPA 60 uM remove 208,869 + -0.8 208,870 - -0.2 209,131 + BT0195 0.21 -0.2 209,279 - BT0195 0.37 +0.2 209,356 - BT0195 0.46 -3.3 209,356 - BT0195 0.46 -2.2 209,400 - BT0195 0.51 -1.8 209,491 - BT0195 0.61 -1.4 209,589 - BT0195 0.72 -2.2 209,589 - BT0195 0.72 +0.2 209,598 + BT0195 0.73 -2.0 209,644 - BT0195 0.78 +1.2 209,803 - +0.2 209,870 - -0.9 209,918 - -4.2 209,997 - BT0196 0.11 -1.4 209,997 - BT0196 0.11 -1.4 209,997 - BT0196 0.11 -1.0 210,000 - BT0196 0.11 -0.8 210,100 + BT0196 0.18 -0.8 210,104 + BT0196 0.18 -1.4 210,148 + BT0196 0.22 -2.7 210,335 + BT0196 0.36 -0.8 210,365 - BT0196 0.38 -2.2 210,385 + BT0196 0.39 -0.8 210,393 + BT0196 0.40 -1.2 210,401 - BT0196 0.41 +0.2 210,468 + BT0196 0.46 +0.2 210,475 - BT0196 0.46 +0.2 210,640 + BT0196 0.58 -0.8 210,641 - BT0196 0.58 -1.2 210,644 + BT0196 0.59 -3.6 210,656 + BT0196 0.59 -1.2 210,755 - BT0196 0.67 -1.4 210,758 - BT0196 0.67 -1.8 210,850 - BT0196 0.74 -1.2 210,856 - BT0196 0.74 -2.8 210,871 + BT0196 0.75 -2.6 210,973 + BT0196 0.83 +0.2 211,028 + BT0196 0.87 -1.8 211,102 - -3.3 211,122 - -1.6 211,375 + +0.2 211,416 + +0.0 211,526 - BT0197 0.11 -2.2 211,551 + BT0197 0.15 +0.2 211,584 + BT0197 0.21 -0.8 211,745 + BT0197 0.47 -1.4 211,842 - BT0197 0.64 -0.8 211,872 - BT0197 0.69 +0.2 211,872 - BT0197 0.69 +0.2 211,930 + BT0197 0.78 -0.8 211,956 + BT0197 0.83 -1.8 212,027 - +0.2 212,071 + +0.4 212,074 + -3.2 212,079 + -1.6 212,089 + +0.2 212,143 - -0.4 212,158 + +0.2
Or see this region's nucleotide sequence