Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS27425

Experiment: RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS27410 and BWI76_RS27415 overlap by 6 nucleotidesBWI76_RS27415 and BWI76_RS27420 are separated by 83 nucleotidesBWI76_RS27420 and BWI76_RS27425 are separated by 54 nucleotidesBWI76_RS27425 and BWI76_RS27430 are separated by 18 nucleotides BWI76_RS27410: BWI76_RS27410 - DNA ligase B, at 5,687,041 to 5,688,717 _RS27410 BWI76_RS27415: BWI76_RS27415 - hypothetical protein, at 5,688,712 to 5,688,891 _RS27415 BWI76_RS27420: BWI76_RS27420 - guanylate kinase, at 5,688,975 to 5,689,598 _RS27420 BWI76_RS27425: BWI76_RS27425 - DNA-directed RNA polymerase subunit omega, at 5,689,653 to 5,689,928 _RS27425 BWI76_RS27430: BWI76_RS27430 - (p)ppGpp synthetase, at 5,689,947 to 5,692,067 _RS27430 Position (kb) 5689 5690Strain fitness (log2 ratio) -2 -1 0 1at 5688.693 kb on - strandat 5688.900 kb on + strandat 5688.900 kb on + strandat 5688.908 kb on - strandat 5688.926 kb on - strandat 5688.926 kb on - strandat 5688.926 kb on - strandat 5689.709 kb on + strand, within BWI76_RS27425at 5689.821 kb on + strand, within BWI76_RS27425at 5689.821 kb on + strand, within BWI76_RS27425

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
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5,688,693 - +0.8
5,688,900 + -0.3
5,688,900 + +0.4
5,688,908 - +0.8
5,688,926 - +1.1
5,688,926 - +1.7
5,688,926 - +0.1
5,689,709 + BWI76_RS27425 0.20 -1.2
5,689,821 + BWI76_RS27425 0.61 -2.2
5,689,821 + BWI76_RS27425 0.61 +0.1

Or see this region's nucleotide sequence