Experiment: RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS24375 and BWI76_RS24380 are separated by 30 nucleotides BWI76_RS24380 and BWI76_RS24385 overlap by 1 nucleotides BWI76_RS24385 and BWI76_RS24390 are separated by 24 nucleotides
BWI76_RS24375: BWI76_RS24375 - DNA topoisomerase IV subunit B, at 5,080,927 to 5,082,822
_RS24375
BWI76_RS24380: BWI76_RS24380 - esterase YqiA, at 5,082,853 to 5,083,428
_RS24380
BWI76_RS24385: BWI76_RS24385 - phosphodiesterase, at 5,083,428 to 5,084,255
_RS24385
BWI76_RS24390: BWI76_RS24390 - hypothetical protein, at 5,084,280 to 5,084,702
_RS24390
Position (kb) 5082
5083
5084 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5083.137 kb on + strand, within BWI76_RS24380 at 5083.179 kb on + strand, within BWI76_RS24380 at 5083.187 kb on - strand, within BWI76_RS24380 at 5083.298 kb on + strand, within BWI76_RS24380 at 5083.306 kb on - strand at 5083.340 kb on - strand, within BWI76_RS24380 at 5083.427 kb on - strand at 5083.522 kb on - strand, within BWI76_RS24385 at 5083.556 kb on + strand, within BWI76_RS24385 at 5083.578 kb on - strand, within BWI76_RS24385 at 5083.578 kb on - strand, within BWI76_RS24385 at 5083.578 kb on - strand, within BWI76_RS24385 at 5083.658 kb on + strand, within BWI76_RS24385 at 5083.726 kb on - strand, within BWI76_RS24385 at 5083.778 kb on + strand, within BWI76_RS24385 at 5083.849 kb on - strand at 5083.945 kb on + strand, within BWI76_RS24385 at 5084.023 kb on + strand, within BWI76_RS24385 at 5084.183 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 5,083,137 + BWI76_RS24380 0.49 -1.8 5,083,179 + BWI76_RS24380 0.57 -0.6 5,083,187 - BWI76_RS24380 0.58 -1.2 5,083,298 + BWI76_RS24380 0.77 +0.2 5,083,306 - +1.2 5,083,340 - BWI76_RS24380 0.85 -2.5 5,083,427 - -2.3 5,083,522 - BWI76_RS24385 0.11 +1.7 5,083,556 + BWI76_RS24385 0.15 -0.8 5,083,578 - BWI76_RS24385 0.18 +0.1 5,083,578 - BWI76_RS24385 0.18 -0.8 5,083,578 - BWI76_RS24385 0.18 -0.3 5,083,658 + BWI76_RS24385 0.28 -0.4 5,083,726 - BWI76_RS24385 0.36 -1.0 5,083,778 + BWI76_RS24385 0.42 +0.3 5,083,849 - +1.2 5,083,945 + BWI76_RS24385 0.62 -2.8 5,084,023 + BWI76_RS24385 0.72 +1.2 5,084,183 + -2.7
Or see this region's nucleotide sequence