Experiment: RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22800 and BWI76_RS22805 are separated by 59 nucleotides BWI76_RS22805 and BWI76_RS22810 are separated by 97 nucleotides
BWI76_RS22800: BWI76_RS22800 - nucleoside triphosphate pyrophosphohydrolase, at 4,753,900 to 4,754,691
_RS22800
BWI76_RS22805: BWI76_RS22805 - GTP diphosphokinase, at 4,754,751 to 4,756,988
_RS22805
BWI76_RS22810: BWI76_RS22810 - 23S rRNA (uracil(1939)-C(5))-methyltransferase, at 4,757,086 to 4,758,429
_RS22810
Position (kb)
4754
4755
4756
4757 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 4753.793 kb on + strand at 4753.793 kb on + strand at 4753.964 kb on - strand at 4754.343 kb on + strand, within BWI76_RS22800 at 4754.483 kb on + strand, within BWI76_RS22800 at 4754.495 kb on + strand, within BWI76_RS22800 at 4754.495 kb on + strand, within BWI76_RS22800 at 4754.512 kb on + strand, within BWI76_RS22800 at 4754.563 kb on - strand, within BWI76_RS22800 at 4754.565 kb on + strand, within BWI76_RS22800 at 4754.573 kb on - strand, within BWI76_RS22800 at 4754.648 kb on + strand at 4754.666 kb on - strand at 4754.825 kb on - strand at 4754.982 kb on - strand, within BWI76_RS22805 at 4755.009 kb on - strand, within BWI76_RS22805 at 4755.251 kb on + strand, within BWI76_RS22805 at 4755.293 kb on - strand, within BWI76_RS22805 at 4755.422 kb on - strand, within BWI76_RS22805 at 4755.424 kb on + strand, within BWI76_RS22805 at 4755.461 kb on - strand, within BWI76_RS22805 at 4755.696 kb on - strand, within BWI76_RS22805 at 4755.724 kb on - strand, within BWI76_RS22805 at 4756.100 kb on + strand, within BWI76_RS22805 at 4756.264 kb on + strand, within BWI76_RS22805 at 4756.303 kb on + strand, within BWI76_RS22805 at 4756.410 kb on - strand, within BWI76_RS22805 at 4756.531 kb on + strand, within BWI76_RS22805 at 4756.598 kb on + strand, within BWI76_RS22805 at 4756.598 kb on + strand, within BWI76_RS22805 at 4756.606 kb on - strand, within BWI76_RS22805 at 4756.661 kb on - strand, within BWI76_RS22805 at 4756.663 kb on + strand, within BWI76_RS22805 at 4756.746 kb on - strand, within BWI76_RS22805 at 4756.775 kb on - strand at 4756.997 kb on + strand at 4756.997 kb on + strand at 4756.997 kb on + strand at 4757.003 kb on - strand at 4757.005 kb on - strand at 4757.005 kb on - strand at 4757.005 kb on - strand at 4757.005 kb on - strand at 4757.042 kb on + strand at 4757.042 kb on - strand at 4757.531 kb on + strand, within BWI76_RS22810 at 4757.637 kb on + strand, within BWI76_RS22810 at 4757.673 kb on + strand, within BWI76_RS22810 at 4757.689 kb on + strand, within BWI76_RS22810 at 4757.945 kb on - strand, within BWI76_RS22810
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 4,753,793 + -0.5 4,753,793 + +0.2 4,753,964 - -1.4 4,754,343 + BWI76_RS22800 0.56 -1.2 4,754,483 + BWI76_RS22800 0.74 -0.1 4,754,495 + BWI76_RS22800 0.75 +0.8 4,754,495 + BWI76_RS22800 0.75 -0.1 4,754,512 + BWI76_RS22800 0.77 -1.6 4,754,563 - BWI76_RS22800 0.84 -1.0 4,754,565 + BWI76_RS22800 0.84 -1.0 4,754,573 - BWI76_RS22800 0.85 -1.9 4,754,648 + +0.2 4,754,666 - +1.0 4,754,825 - -4.6 4,754,982 - BWI76_RS22805 0.10 -0.5 4,755,009 - BWI76_RS22805 0.12 -2.4 4,755,251 + BWI76_RS22805 0.22 -2.1 4,755,293 - BWI76_RS22805 0.24 -1.8 4,755,422 - BWI76_RS22805 0.30 -1.4 4,755,424 + BWI76_RS22805 0.30 -2.3 4,755,461 - BWI76_RS22805 0.32 -0.0 4,755,696 - BWI76_RS22805 0.42 -0.3 4,755,724 - BWI76_RS22805 0.43 -1.8 4,756,100 + BWI76_RS22805 0.60 -3.6 4,756,264 + BWI76_RS22805 0.68 -4.5 4,756,303 + BWI76_RS22805 0.69 -4.8 4,756,410 - BWI76_RS22805 0.74 -3.7 4,756,531 + BWI76_RS22805 0.80 -3.6 4,756,598 + BWI76_RS22805 0.83 -2.5 4,756,598 + BWI76_RS22805 0.83 -2.4 4,756,606 - BWI76_RS22805 0.83 -3.8 4,756,661 - BWI76_RS22805 0.85 -5.0 4,756,663 + BWI76_RS22805 0.85 -2.2 4,756,746 - BWI76_RS22805 0.89 -3.0 4,756,775 - -1.3 4,756,997 + -1.1 4,756,997 + -1.8 4,756,997 + -2.6 4,757,003 - +0.2 4,757,005 - -0.4 4,757,005 - -1.0 4,757,005 - +0.9 4,757,005 - -2.2 4,757,042 + -3.7 4,757,042 - -1.1 4,757,531 + BWI76_RS22810 0.33 -4.0 4,757,637 + BWI76_RS22810 0.41 -1.2 4,757,673 + BWI76_RS22810 0.44 +0.3 4,757,689 + BWI76_RS22810 0.45 -2.2 4,757,945 - BWI76_RS22810 0.64 -2.2
Or see this region's nucleotide sequence