Experiment: RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22675 and BWI76_RS22680 are separated by 19 nucleotides BWI76_RS22680 and BWI76_RS22685 are separated by 139 nucleotides BWI76_RS22685 and BWI76_RS22690 are separated by 53 nucleotides BWI76_RS22690 and BWI76_RS22695 overlap by 1 nucleotides
BWI76_RS22675: BWI76_RS22675 - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, at 4,725,382 to 4,726,092
_RS22675
BWI76_RS22680: BWI76_RS22680 - cell division protein FtsB, at 4,726,112 to 4,726,429
_RS22680
BWI76_RS22685: BWI76_RS22685 - hypothetical protein, at 4,726,569 to 4,726,895
_RS22685
BWI76_RS22690: BWI76_RS22690 - adenylyl-sulfate kinase, at 4,726,949 to 4,727,554
_RS22690
BWI76_RS22695: BWI76_RS22695 - sulfate adenylyltransferase, at 4,727,554 to 4,728,981
_RS22695
Position (kb) 4726
4727
4728 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4726.585 kb on + strand at 4726.723 kb on + strand, within BWI76_RS22685 at 4726.723 kb on + strand, within BWI76_RS22685 at 4726.730 kb on - strand, within BWI76_RS22685 at 4726.731 kb on - strand, within BWI76_RS22685 at 4726.731 kb on - strand, within BWI76_RS22685 at 4726.731 kb on - strand, within BWI76_RS22685 at 4726.731 kb on - strand, within BWI76_RS22685 at 4726.753 kb on + strand, within BWI76_RS22685 at 4726.776 kb on - strand, within BWI76_RS22685 at 4726.817 kb on + strand, within BWI76_RS22685 at 4726.909 kb on + strand at 4726.964 kb on - strand at 4726.980 kb on - strand at 4726.980 kb on - strand at 4727.091 kb on + strand, within BWI76_RS22690 at 4727.106 kb on + strand, within BWI76_RS22690 at 4727.212 kb on + strand, within BWI76_RS22690 at 4727.279 kb on - strand, within BWI76_RS22690 at 4727.339 kb on + strand, within BWI76_RS22690 at 4727.508 kb on - strand at 4727.638 kb on + strand at 4727.638 kb on + strand at 4727.638 kb on + strand at 4727.638 kb on + strand at 4727.638 kb on + strand at 4727.643 kb on + strand at 4727.646 kb on - strand at 4727.646 kb on - strand at 4727.646 kb on - strand at 4727.697 kb on + strand, within BWI76_RS22695 at 4728.156 kb on + strand at 4728.156 kb on + strand, within BWI76_RS22695 at 4728.164 kb on - strand, within BWI76_RS22695 at 4728.164 kb on - strand, within BWI76_RS22695 at 4728.413 kb on - strand, within BWI76_RS22695
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 4,726,585 + -2.9 4,726,723 + BWI76_RS22685 0.47 -0.1 4,726,723 + BWI76_RS22685 0.47 -2.6 4,726,730 - BWI76_RS22685 0.49 -0.4 4,726,731 - BWI76_RS22685 0.50 -0.3 4,726,731 - BWI76_RS22685 0.50 -0.4 4,726,731 - BWI76_RS22685 0.50 -0.1 4,726,731 - BWI76_RS22685 0.50 -0.0 4,726,753 + BWI76_RS22685 0.56 -0.0 4,726,776 - BWI76_RS22685 0.63 +1.6 4,726,817 + BWI76_RS22685 0.76 -0.3 4,726,909 + +0.2 4,726,964 - -2.0 4,726,980 - -2.1 4,726,980 - -1.0 4,727,091 + BWI76_RS22690 0.23 -2.0 4,727,106 + BWI76_RS22690 0.26 -1.8 4,727,212 + BWI76_RS22690 0.43 +1.0 4,727,279 - BWI76_RS22690 0.54 -1.8 4,727,339 + BWI76_RS22690 0.64 -3.3 4,727,508 - -2.7 4,727,638 + -1.2 4,727,638 + -0.1 4,727,638 + +0.0 4,727,638 + -0.0 4,727,638 + -0.4 4,727,643 + -3.0 4,727,646 - -1.2 4,727,646 - +1.5 4,727,646 - +0.4 4,727,697 + BWI76_RS22695 0.10 +0.7 4,728,156 + -0.0 4,728,156 + BWI76_RS22695 0.42 -0.9 4,728,164 - BWI76_RS22695 0.43 -0.7 4,728,164 - BWI76_RS22695 0.43 -3.3 4,728,413 - BWI76_RS22695 0.60 -0.0
Or see this region's nucleotide sequence