Experiment: RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS21225 and BWI76_RS21230 overlap by 14 nucleotides BWI76_RS21230 and BWI76_RS21235 overlap by 15 nucleotides BWI76_RS21235 and BWI76_RS21240 overlap by 56 nucleotides
BWI76_RS21225: BWI76_RS21225 - serine hydroxymethyltransferase, at 4,445,622 to 4,446,875
_RS21225
BWI76_RS21230: BWI76_RS21230 - hypothetical protein, at 4,446,862 to 4,447,091
_RS21230
BWI76_RS21235: BWI76_RS21235 - hypothetical protein, at 4,447,077 to 4,447,256
_RS21235
BWI76_RS21240: BWI76_RS21240 - flavohemoprotein, at 4,447,201 to 4,448,391
_RS21240
Position (kb) 4447
4448 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4446.973 kb on - strand, within BWI76_RS21230 at 4447.198 kb on + strand, within BWI76_RS21235 at 4447.259 kb on - strand at 4447.357 kb on - strand, within BWI76_RS21240 at 4447.482 kb on + strand, within BWI76_RS21240 at 4447.482 kb on + strand, within BWI76_RS21240 at 4447.584 kb on - strand, within BWI76_RS21240 at 4447.854 kb on - strand, within BWI76_RS21240 at 4448.039 kb on - strand, within BWI76_RS21240 at 4448.094 kb on - strand, within BWI76_RS21240 at 4448.109 kb on - strand, within BWI76_RS21240 at 4448.201 kb on - strand, within BWI76_RS21240
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 4,446,973 - BWI76_RS21230 0.48 +0.9 4,447,198 + BWI76_RS21235 0.67 -2.9 4,447,259 - -0.2 4,447,357 - BWI76_RS21240 0.13 +1.2 4,447,482 + BWI76_RS21240 0.24 -0.2 4,447,482 + BWI76_RS21240 0.24 +0.2 4,447,584 - BWI76_RS21240 0.32 -0.2 4,447,854 - BWI76_RS21240 0.55 +2.0 4,448,039 - BWI76_RS21240 0.70 +0.7 4,448,094 - BWI76_RS21240 0.75 +0.7 4,448,109 - BWI76_RS21240 0.76 -0.4 4,448,201 - BWI76_RS21240 0.84 +0.2
Or see this region's nucleotide sequence