Experiment: RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16905 and BWI76_RS16910 overlap by 8 nucleotides BWI76_RS16910 and BWI76_RS16915 are separated by 9 nucleotides BWI76_RS16915 and BWI76_RS16920 are separated by 17 nucleotides BWI76_RS16920 and BWI76_RS16925 are separated by 1 nucleotides
BWI76_RS16905: BWI76_RS16905 - acetaldehyde dehydrogenase (acetylating), at 3,505,611 to 3,506,561
_RS16905
BWI76_RS16910: BWI76_RS16910 - 2-keto-4-pentenoate hydratase, at 3,506,554 to 3,507,360
_RS16910
BWI76_RS16915: BWI76_RS16915 - alpha/beta hydrolase fold protein, at 3,507,370 to 3,508,236
_RS16915
BWI76_RS16920: BWI76_RS16920 - 3-(2,3-dihydroxyphenyl)propionate dioxygenase, at 3,508,254 to 3,509,198
_RS16920
BWI76_RS16925: BWI76_RS16925 - 3-(3-hydroxyphenyl)propionate hydroxylase, at 3,509,200 to 3,510,867
_RS16925
Position (kb)
3507
3508
3509 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3506.575 kb on - strand at 3506.594 kb on - strand at 3506.986 kb on - strand, within BWI76_RS16910 at 3507.250 kb on - strand, within BWI76_RS16910 at 3507.409 kb on + strand at 3507.410 kb on - strand at 3507.823 kb on - strand, within BWI76_RS16915 at 3507.854 kb on + strand, within BWI76_RS16915 at 3508.140 kb on + strand, within BWI76_RS16915 at 3508.418 kb on + strand, within BWI76_RS16920 at 3508.568 kb on + strand, within BWI76_RS16920 at 3509.124 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 3,506,575 - +0.5 3,506,594 - -0.2 3,506,986 - BWI76_RS16910 0.54 -0.2 3,507,250 - BWI76_RS16910 0.86 +1.5 3,507,409 + +1.9 3,507,410 - +0.3 3,507,823 - BWI76_RS16915 0.52 +0.1 3,507,854 + BWI76_RS16915 0.56 +0.1 3,508,140 + BWI76_RS16915 0.89 +1.0 3,508,418 + BWI76_RS16920 0.17 -2.2 3,508,568 + BWI76_RS16920 0.33 -1.5 3,509,124 + +0.9
Or see this region's nucleotide sequence