Experiment: RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15340 and BWI76_RS15345 are separated by 176 nucleotides BWI76_RS15345 and BWI76_RS15350 are separated by 149 nucleotides BWI76_RS15350 and BWI76_RS15355 are separated by 67 nucleotides
BWI76_RS15340: BWI76_RS15340 - urea ABC transporter substrate-binding protein, at 3,212,576 to 3,213,847
_RS15340
BWI76_RS15345: BWI76_RS15345 - 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase, at 3,214,024 to 3,215,127
_RS15345
BWI76_RS15350: BWI76_RS15350 - hypothetical protein, at 3,215,277 to 3,215,669
_RS15350
BWI76_RS15355: BWI76_RS15355 - transcriptional regulator FtrA, at 3,215,737 to 3,216,747
_RS15355
Position (kb)
3214
3215
3216 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3213.110 kb on - strand, within BWI76_RS15340 at 3213.476 kb on - strand, within BWI76_RS15340 at 3213.488 kb on - strand, within BWI76_RS15340 at 3213.794 kb on + strand at 3213.795 kb on - strand at 3213.797 kb on + strand at 3213.856 kb on + strand at 3213.856 kb on + strand at 3213.856 kb on + strand at 3213.864 kb on - strand at 3213.864 kb on - strand at 3213.957 kb on - strand at 3214.012 kb on + strand at 3214.033 kb on - strand at 3214.040 kb on - strand at 3214.159 kb on - strand, within BWI76_RS15345 at 3214.451 kb on + strand, within BWI76_RS15345 at 3214.489 kb on + strand, within BWI76_RS15345 at 3214.554 kb on - strand, within BWI76_RS15345 at 3214.844 kb on - strand, within BWI76_RS15345 at 3215.104 kb on + strand at 3215.295 kb on + strand at 3215.297 kb on - strand at 3215.303 kb on - strand at 3215.354 kb on - strand, within BWI76_RS15350 at 3216.042 kb on + strand, within BWI76_RS15355 at 3216.052 kb on + strand, within BWI76_RS15355
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 3,213,110 - BWI76_RS15340 0.42 +0.5 3,213,476 - BWI76_RS15340 0.71 -1.5 3,213,488 - BWI76_RS15340 0.72 -3.2 3,213,794 + -0.8 3,213,795 - +0.2 3,213,797 + +0.3 3,213,856 + +0.8 3,213,856 + +2.1 3,213,856 + -0.0 3,213,864 - +0.9 3,213,864 - +0.2 3,213,957 - +0.2 3,214,012 + -2.2 3,214,033 - -0.4 3,214,040 - +0.4 3,214,159 - BWI76_RS15345 0.12 -0.1 3,214,451 + BWI76_RS15345 0.39 -3.3 3,214,489 + BWI76_RS15345 0.42 -0.6 3,214,554 - BWI76_RS15345 0.48 +1.2 3,214,844 - BWI76_RS15345 0.74 +0.1 3,215,104 + -0.3 3,215,295 + +0.9 3,215,297 - +1.3 3,215,303 - -0.7 3,215,354 - BWI76_RS15350 0.20 -3.4 3,216,042 + BWI76_RS15355 0.30 -0.4 3,216,052 + BWI76_RS15355 0.31 +0.2
Or see this region's nucleotide sequence