Experiment: RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13990 and BWI76_RS13995 are separated by 184 nucleotides BWI76_RS13995 and BWI76_RS14000 are separated by 103 nucleotides BWI76_RS14000 and BWI76_RS14005 are separated by 2 nucleotides
BWI76_RS13990: BWI76_RS13990 - allophanate hydrolase, at 2,921,893 to 2,923,689
_RS13990
BWI76_RS13995: BWI76_RS13995 - penicillin-binding protein 2, at 2,923,874 to 2,925,793
_RS13995
BWI76_RS14000: BWI76_RS14000 - glutamine amidotransferase, at 2,925,897 to 2,926,640
_RS14000
BWI76_RS14005: BWI76_RS14005 - TetR family transcriptional regulator, at 2,926,643 to 2,927,191
_RS14005
Position (kb)
2923
2924
2925
2926 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2922.966 kb on + strand, within BWI76_RS13990 at 2923.183 kb on - strand, within BWI76_RS13990 at 2923.518 kb on - strand at 2924.282 kb on - strand, within BWI76_RS13995 at 2924.454 kb on - strand, within BWI76_RS13995 at 2924.576 kb on + strand, within BWI76_RS13995 at 2924.944 kb on + strand, within BWI76_RS13995 at 2925.323 kb on - strand, within BWI76_RS13995 at 2925.419 kb on - strand, within BWI76_RS13995 at 2925.519 kb on - strand, within BWI76_RS13995 at 2926.067 kb on + strand, within BWI76_RS14000 at 2926.339 kb on - strand, within BWI76_RS14000 at 2926.343 kb on + strand, within BWI76_RS14000 at 2926.523 kb on - strand, within BWI76_RS14000 at 2926.570 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 2,922,966 + BWI76_RS13990 0.60 +0.6 2,923,183 - BWI76_RS13990 0.72 +0.3 2,923,518 - +2.2 2,924,282 - BWI76_RS13995 0.21 -3.3 2,924,454 - BWI76_RS13995 0.30 -1.5 2,924,576 + BWI76_RS13995 0.37 +0.1 2,924,944 + BWI76_RS13995 0.56 -1.3 2,925,323 - BWI76_RS13995 0.75 -0.5 2,925,419 - BWI76_RS13995 0.80 -0.6 2,925,519 - BWI76_RS13995 0.86 +0.3 2,926,067 + BWI76_RS14000 0.23 +0.4 2,926,339 - BWI76_RS14000 0.59 -0.6 2,926,343 + BWI76_RS14000 0.60 +0.3 2,926,523 - BWI76_RS14000 0.84 -0.9 2,926,570 + +0.3
Or see this region's nucleotide sequence