Experiment: RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12450 and BWI76_RS12455 are separated by 51 nucleotides BWI76_RS12455 and BWI76_RS12460 overlap by 1 nucleotides BWI76_RS12460 and BWI76_RS12465 are separated by 19 nucleotides BWI76_RS12465 and BWI76_RS12470 are separated by 33 nucleotides BWI76_RS12470 and BWI76_RS12475 are separated by 100 nucleotides
BWI76_RS12450: BWI76_RS12450 - phage shock protein PspA, at 2,602,661 to 2,603,329
_RS12450
BWI76_RS12455: BWI76_RS12455 - envelope stress response membrane protein PspB, at 2,603,381 to 2,603,605
_RS12455
BWI76_RS12460: BWI76_RS12460 - envelope stress response membrane protein PspC, at 2,603,605 to 2,603,964
_RS12460
BWI76_RS12465: BWI76_RS12465 - phage shock protein D, at 2,603,984 to 2,604,214
_RS12465
BWI76_RS12470: BWI76_RS12470 - thiosulfate sulfurtransferase PspE, at 2,604,248 to 2,604,562
_RS12470
BWI76_RS12475: BWI76_RS12475 - hypothetical protein, at 2,604,663 to 2,606,060
_RS12475
Position (kb)
2604
2605 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2603.698 kb on - strand, within BWI76_RS12460 at 2603.731 kb on - strand, within BWI76_RS12460 at 2604.075 kb on - strand, within BWI76_RS12465 at 2604.208 kb on + strand at 2604.307 kb on + strand, within BWI76_RS12470 at 2604.762 kb on + strand at 2604.764 kb on + strand at 2604.787 kb on - strand at 2604.787 kb on - strand at 2604.931 kb on - strand, within BWI76_RS12475 at 2605.111 kb on - strand, within BWI76_RS12475 at 2605.161 kb on - strand, within BWI76_RS12475 at 2605.223 kb on - strand, within BWI76_RS12475 at 2605.375 kb on + strand, within BWI76_RS12475 at 2605.385 kb on + strand, within BWI76_RS12475
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 2,603,698 - BWI76_RS12460 0.26 +1.1 2,603,731 - BWI76_RS12460 0.35 -1.2 2,604,075 - BWI76_RS12465 0.39 +0.1 2,604,208 + +0.5 2,604,307 + BWI76_RS12470 0.19 -1.5 2,604,762 + +0.7 2,604,764 + -1.2 2,604,787 - -2.7 2,604,787 - +0.6 2,604,931 - BWI76_RS12475 0.19 -3.1 2,605,111 - BWI76_RS12475 0.32 +1.0 2,605,161 - BWI76_RS12475 0.36 -0.9 2,605,223 - BWI76_RS12475 0.40 +0.4 2,605,375 + BWI76_RS12475 0.51 +0.2 2,605,385 + BWI76_RS12475 0.52 +0.8
Or see this region's nucleotide sequence