Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS12450

Experiment: RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS12445 and BWI76_RS12450 are separated by 167 nucleotidesBWI76_RS12450 and BWI76_RS12455 are separated by 51 nucleotidesBWI76_RS12455 and BWI76_RS12460 overlap by 1 nucleotidesBWI76_RS12460 and BWI76_RS12465 are separated by 19 nucleotidesBWI76_RS12465 and BWI76_RS12470 are separated by 33 nucleotides BWI76_RS12445: BWI76_RS12445 - phage shock protein operon transcriptional activator, at 2,601,519 to 2,602,493 _RS12445 BWI76_RS12450: BWI76_RS12450 - phage shock protein PspA, at 2,602,661 to 2,603,329 _RS12450 BWI76_RS12455: BWI76_RS12455 - envelope stress response membrane protein PspB, at 2,603,381 to 2,603,605 _RS12455 BWI76_RS12460: BWI76_RS12460 - envelope stress response membrane protein PspC, at 2,603,605 to 2,603,964 _RS12460 BWI76_RS12465: BWI76_RS12465 - phage shock protein D, at 2,603,984 to 2,604,214 _RS12465 BWI76_RS12470: BWI76_RS12470 - thiosulfate sulfurtransferase PspE, at 2,604,248 to 2,604,562 _RS12470 Position (kb) 2602 2603 2604Strain fitness (log2 ratio) -3 -2 -1 0 1at 2601.837 kb on + strand, within BWI76_RS12445at 2601.993 kb on + strand, within BWI76_RS12445at 2602.265 kb on + strand, within BWI76_RS12445at 2602.423 kb on + strandat 2602.826 kb on + strand, within BWI76_RS12450at 2603.123 kb on + strand, within BWI76_RS12450at 2603.698 kb on - strand, within BWI76_RS12460at 2603.731 kb on - strand, within BWI76_RS12460at 2604.075 kb on - strand, within BWI76_RS12465at 2604.208 kb on + strandat 2604.307 kb on + strand, within BWI76_RS12470

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
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2,601,837 + BWI76_RS12445 0.33 +0.4
2,601,993 + BWI76_RS12445 0.49 -3.1
2,602,265 + BWI76_RS12445 0.77 +0.6
2,602,423 + -0.1
2,602,826 + BWI76_RS12450 0.25 -2.7
2,603,123 + BWI76_RS12450 0.69 -1.4
2,603,698 - BWI76_RS12460 0.26 +1.1
2,603,731 - BWI76_RS12460 0.35 -1.2
2,604,075 - BWI76_RS12465 0.39 +0.1
2,604,208 + +0.5
2,604,307 + BWI76_RS12470 0.19 -1.5

Or see this region's nucleotide sequence