Experiment: RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12400 and BWI76_RS12405 are separated by 509 nucleotides BWI76_RS12405 and BWI76_RS12410 are separated by 227 nucleotides BWI76_RS12410 and BWI76_RS12415 are separated by 204 nucleotides
BWI76_RS12400: BWI76_RS12400 - TetR family transcriptional regulator, at 2,589,961 to 2,590,617
_RS12400
BWI76_RS12405: BWI76_RS12405 - diguanylate phosphodiesterase, at 2,591,127 to 2,592,299
_RS12405
BWI76_RS12410: BWI76_RS12410 - hypothetical protein, at 2,592,527 to 2,592,838
_RS12410
BWI76_RS12415: BWI76_RS12415 - antimicrobial peptide ABC transporter permease SapB, at 2,593,043 to 2,594,008
_RS12415
Position (kb)
2591
2592
2593 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2590.201 kb on - strand, within BWI76_RS12400 at 2590.238 kb on - strand, within BWI76_RS12400 at 2590.340 kb on - strand, within BWI76_RS12400 at 2590.353 kb on - strand, within BWI76_RS12400 at 2590.362 kb on - strand at 2590.407 kb on - strand, within BWI76_RS12400 at 2590.524 kb on + strand, within BWI76_RS12400 at 2590.532 kb on - strand, within BWI76_RS12400 at 2590.532 kb on - strand, within BWI76_RS12400 at 2590.532 kb on - strand, within BWI76_RS12400 at 2590.532 kb on - strand, within BWI76_RS12400 at 2590.532 kb on - strand, within BWI76_RS12400 at 2590.613 kb on + strand at 2590.621 kb on - strand at 2590.621 kb on - strand at 2590.679 kb on - strand at 2590.743 kb on + strand at 2590.751 kb on - strand at 2590.751 kb on - strand at 2590.751 kb on - strand at 2590.751 kb on - strand at 2590.757 kb on + strand at 2590.765 kb on - strand at 2590.775 kb on - strand at 2590.775 kb on - strand at 2590.775 kb on - strand at 2590.775 kb on - strand at 2590.779 kb on - strand at 2590.781 kb on - strand at 2590.791 kb on - strand at 2590.801 kb on + strand at 2590.814 kb on + strand at 2590.822 kb on - strand at 2590.822 kb on - strand at 2590.822 kb on - strand at 2590.822 kb on - strand at 2590.822 kb on - strand at 2590.824 kb on + strand at 2590.835 kb on + strand at 2590.859 kb on + strand at 2590.931 kb on + strand at 2590.939 kb on + strand at 2590.939 kb on + strand at 2590.940 kb on - strand at 2590.940 kb on - strand at 2590.948 kb on + strand at 2590.956 kb on - strand at 2590.961 kb on - strand at 2590.977 kb on + strand at 2591.012 kb on + strand at 2591.015 kb on + strand at 2591.023 kb on + strand at 2591.023 kb on + strand at 2591.023 kb on + strand at 2591.023 kb on + strand at 2591.023 kb on + strand at 2591.023 kb on + strand at 2591.023 kb on + strand at 2591.023 kb on + strand at 2591.023 kb on + strand at 2591.023 kb on + strand at 2591.023 kb on + strand at 2591.023 kb on + strand at 2591.023 kb on + strand at 2591.031 kb on - strand at 2591.031 kb on - strand at 2591.031 kb on - strand at 2591.031 kb on - strand at 2591.031 kb on - strand at 2591.031 kb on - strand at 2591.031 kb on - strand at 2591.037 kb on + strand at 2591.037 kb on + strand at 2591.037 kb on + strand at 2591.044 kb on - strand at 2591.079 kb on + strand at 2591.159 kb on - strand at 2591.179 kb on - strand at 2591.216 kb on - strand at 2591.229 kb on - strand at 2591.235 kb on - strand at 2591.277 kb on + strand, within BWI76_RS12405 at 2591.285 kb on - strand, within BWI76_RS12405 at 2591.312 kb on - strand at 2591.400 kb on + strand, within BWI76_RS12405 at 2591.400 kb on + strand, within BWI76_RS12405 at 2591.400 kb on + strand, within BWI76_RS12405 at 2591.400 kb on + strand, within BWI76_RS12405 at 2591.623 kb on + strand at 2591.728 kb on + strand, within BWI76_RS12405 at 2591.906 kb on - strand, within BWI76_RS12405 at 2591.906 kb on - strand, within BWI76_RS12405 at 2591.954 kb on + strand, within BWI76_RS12405 at 2591.957 kb on - strand, within BWI76_RS12405 at 2591.962 kb on - strand at 2591.962 kb on - strand, within BWI76_RS12405 at 2591.975 kb on - strand, within BWI76_RS12405 at 2591.988 kb on + strand at 2591.990 kb on + strand, within BWI76_RS12405 at 2592.042 kb on - strand, within BWI76_RS12405 at 2592.045 kb on + strand, within BWI76_RS12405 at 2592.053 kb on - strand, within BWI76_RS12405 at 2592.132 kb on + strand, within BWI76_RS12405 at 2592.134 kb on + strand, within BWI76_RS12405 at 2592.135 kb on - strand, within BWI76_RS12405 at 2592.266 kb on + strand at 2592.269 kb on - strand at 2592.269 kb on - strand at 2592.497 kb on + strand at 2592.530 kb on + strand at 2592.535 kb on + strand at 2592.566 kb on + strand, within BWI76_RS12410 at 2592.589 kb on - strand, within BWI76_RS12410 at 2592.627 kb on + strand, within BWI76_RS12410 at 2592.627 kb on + strand, within BWI76_RS12410 at 2592.641 kb on + strand, within BWI76_RS12410 at 2592.641 kb on + strand, within BWI76_RS12410 at 2592.641 kb on + strand, within BWI76_RS12410 at 2592.648 kb on - strand, within BWI76_RS12410 at 2592.649 kb on - strand, within BWI76_RS12410 at 2592.656 kb on - strand, within BWI76_RS12410 at 2592.672 kb on + strand, within BWI76_RS12410 at 2592.672 kb on + strand, within BWI76_RS12410 at 2592.726 kb on - strand, within BWI76_RS12410 at 2592.794 kb on - strand, within BWI76_RS12410 at 2592.794 kb on - strand, within BWI76_RS12410 at 2592.828 kb on + strand at 2592.851 kb on - strand at 2592.873 kb on - strand at 2592.874 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 2,590,201 - BWI76_RS12400 0.37 -0.9 2,590,238 - BWI76_RS12400 0.42 +0.9 2,590,340 - BWI76_RS12400 0.58 -1.2 2,590,353 - BWI76_RS12400 0.60 +0.5 2,590,362 - +0.7 2,590,407 - BWI76_RS12400 0.68 -2.2 2,590,524 + BWI76_RS12400 0.86 +1.4 2,590,532 - BWI76_RS12400 0.87 +0.7 2,590,532 - BWI76_RS12400 0.87 +0.1 2,590,532 - BWI76_RS12400 0.87 +1.1 2,590,532 - BWI76_RS12400 0.87 -0.5 2,590,532 - BWI76_RS12400 0.87 +0.1 2,590,613 + -3.2 2,590,621 - +1.1 2,590,621 - -0.4 2,590,679 - -1.2 2,590,743 + -0.5 2,590,751 - -0.9 2,590,751 - +0.9 2,590,751 - -2.2 2,590,751 - -2.2 2,590,757 + -1.5 2,590,765 - -2.9 2,590,775 - -2.9 2,590,775 - -2.6 2,590,775 - -1.3 2,590,775 - +0.7 2,590,779 - -1.9 2,590,781 - +0.9 2,590,791 - -2.2 2,590,801 + +0.1 2,590,814 + -1.7 2,590,822 - -1.4 2,590,822 - -1.5 2,590,822 - -0.8 2,590,822 - -1.5 2,590,822 - +1.4 2,590,824 + -0.9 2,590,835 + -1.2 2,590,859 + -0.3 2,590,931 + -1.7 2,590,939 + +0.1 2,590,939 + +1.9 2,590,940 - -1.2 2,590,940 - +1.1 2,590,948 + -2.8 2,590,956 - +0.1 2,590,961 - -1.7 2,590,977 + +1.1 2,591,012 + +0.1 2,591,015 + +0.4 2,591,023 + -1.5 2,591,023 + -0.6 2,591,023 + +0.5 2,591,023 + -0.9 2,591,023 + -0.4 2,591,023 + -2.8 2,591,023 + -2.2 2,591,023 + -0.7 2,591,023 + +0.6 2,591,023 + -3.3 2,591,023 + -0.4 2,591,023 + -3.2 2,591,023 + -1.2 2,591,031 - +1.1 2,591,031 - -0.9 2,591,031 - +1.7 2,591,031 - +0.4 2,591,031 - -0.5 2,591,031 - -0.5 2,591,031 - -1.7 2,591,037 + -2.5 2,591,037 + +0.2 2,591,037 + -1.1 2,591,044 - -2.2 2,591,079 + -0.4 2,591,159 - -3.3 2,591,179 - -1.7 2,591,216 - -3.6 2,591,229 - -2.3 2,591,235 - -2.5 2,591,277 + BWI76_RS12405 0.13 -1.4 2,591,285 - BWI76_RS12405 0.13 -0.9 2,591,312 - -0.5 2,591,400 + BWI76_RS12405 0.23 -1.5 2,591,400 + BWI76_RS12405 0.23 -1.5 2,591,400 + BWI76_RS12405 0.23 -0.9 2,591,400 + BWI76_RS12405 0.23 -0.5 2,591,623 + +0.1 2,591,728 + BWI76_RS12405 0.51 -0.5 2,591,906 - BWI76_RS12405 0.66 -0.3 2,591,906 - BWI76_RS12405 0.66 +0.4 2,591,954 + BWI76_RS12405 0.71 -2.0 2,591,957 - BWI76_RS12405 0.71 +0.2 2,591,962 - -0.5 2,591,962 - BWI76_RS12405 0.71 -2.7 2,591,975 - BWI76_RS12405 0.72 -0.9 2,591,988 + -0.2 2,591,990 + BWI76_RS12405 0.74 -2.3 2,592,042 - BWI76_RS12405 0.78 -2.8 2,592,045 + BWI76_RS12405 0.78 -2.8 2,592,053 - BWI76_RS12405 0.79 -2.5 2,592,132 + BWI76_RS12405 0.86 +1.1 2,592,134 + BWI76_RS12405 0.86 -1.5 2,592,135 - BWI76_RS12405 0.86 -0.9 2,592,266 + -1.2 2,592,269 - +0.2 2,592,269 - -0.3 2,592,497 + -1.1 2,592,530 + +0.2 2,592,535 + +0.2 2,592,566 + BWI76_RS12410 0.12 +0.2 2,592,589 - BWI76_RS12410 0.20 -2.2 2,592,627 + BWI76_RS12410 0.32 +0.2 2,592,627 + BWI76_RS12410 0.32 -0.6 2,592,641 + BWI76_RS12410 0.37 -1.7 2,592,641 + BWI76_RS12410 0.37 -0.7 2,592,641 + BWI76_RS12410 0.37 -3.8 2,592,648 - BWI76_RS12410 0.39 -0.0 2,592,649 - BWI76_RS12410 0.39 -0.9 2,592,656 - BWI76_RS12410 0.41 +1.1 2,592,672 + BWI76_RS12410 0.46 -1.5 2,592,672 + BWI76_RS12410 0.46 +0.0 2,592,726 - BWI76_RS12410 0.64 -3.2 2,592,794 - BWI76_RS12410 0.86 -1.2 2,592,794 - BWI76_RS12410 0.86 -0.1 2,592,828 + -1.7 2,592,851 - -1.9 2,592,873 - +2.4 2,592,874 - +0.5
Or see this region's nucleotide sequence