Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS12340

Experiment: RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
2,574,459	+		BWI76_RS12330	0.49	+1.0
2,574,668	+				-0.8
2,574,668	+				-0.6
2,574,675	-				+0.1
2,574,722	+				-0.3
2,574,827	+		BWI76_RS12335	0.29	-1.6
2,574,835	-		BWI76_RS12335	0.30	-2.0
2,574,843	-		BWI76_RS12335	0.32	-1.2
2,574,862	-		BWI76_RS12335	0.36	-1.8
2,575,011	-				+1.1
2,575,034	-		BWI76_RS12335	0.72	-3.3
2,575,034	-		BWI76_RS12335	0.72	-0.3
2,575,075	-		BWI76_RS12335	0.80	+0.4
2,575,087	+		BWI76_RS12335	0.83	-1.2
2,575,087	+		BWI76_RS12335	0.83	-1.4
2,575,091	+		BWI76_RS12335	0.83	+1.1
2,575,091	+		BWI76_RS12335	0.83	+0.2
2,575,095	-		BWI76_RS12335	0.84	-0.8
2,575,095	-		BWI76_RS12335	0.84	-2.0
2,575,099	-		BWI76_RS12335	0.85	+1.4
2,575,099	-		BWI76_RS12335	0.85	-0.0
2,575,099	-		BWI76_RS12335	0.85	+2.0
2,575,099	-		BWI76_RS12335	0.85	-1.6
2,575,115	+		BWI76_RS12335	0.88	-2.2
2,575,119	-		BWI76_RS12335	0.89	-1.4
2,575,125	-				-0.4
2,575,179	+				-1.2
2,575,186	-				+1.7
2,575,312	+				-3.6
2,575,322	+				-1.7
2,575,322	+				-2.0
2,575,322	+				-0.9
2,575,329	+				-3.6
2,575,329	+				+0.5
2,575,329	+				-0.7
2,575,329	+				-2.7
2,575,335	+				-1.9
2,575,335	+				-0.9
2,575,337	-				+0.1
2,575,355	-				-0.8
2,575,416	-				-0.0
2,575,424	-				+0.5
2,575,464	-				-0.9
2,575,585	+				-0.9
2,575,702	+				-2.7
2,575,758	+		BWI76_RS12340	0.41	-3.5
2,575,860	+		BWI76_RS12340	0.53	+1.1
2,575,861	-		BWI76_RS12340	0.53	-1.2
2,576,142	+		BWI76_RS12340	0.85	-1.7
2,576,166	-		BWI76_RS12340	0.87	-2.0
2,576,168	+		BWI76_RS12340	0.88	-0.6
2,576,244	+				+0.1
2,576,244	+				+0.1
2,576,249	+				-0.1
2,576,259	+				-2.9
2,576,274	+				-1.1
2,576,274	+				+0.1
2,576,287	+				+0.5
2,576,300	+				-2.3
2,576,300	+				+2.0
2,576,301	-				-2.6
2,576,305	+				-1.9
2,576,305	+				-0.4
2,576,305	+				-0.3
2,576,305	+				-2.9
2,576,318	-				+1.7
2,576,322	+				-1.9
2,576,322	+				+0.1
2,576,359	+				-1.6
2,576,616	-		BWI76_RS12345	0.58	+0.6
2,576,656	-		BWI76_RS12345	0.68	-0.2
2,576,826	+				-0.1
2,576,867	+				-0.7
2,577,120	+		BWI76_RS12350	0.11	-1.8
2,577,120	+		BWI76_RS12350	0.11	-1.1
2,577,206	+		BWI76_RS12350	0.14	+0.1
2,577,206	+		BWI76_RS12350	0.14	+0.2