Experiment: RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11810 and BWI76_RS11815 are separated by 97 nucleotides BWI76_RS11815 and BWI76_RS11820 are separated by 214 nucleotides BWI76_RS11820 and BWI76_RS11825 are separated by 121 nucleotides
BWI76_RS11810: BWI76_RS11810 - outer membrane protein OmpW, at 2,484,371 to 2,485,009
_RS11810
BWI76_RS11815: BWI76_RS11815 - hypothetical protein, at 2,485,107 to 2,485,352
_RS11815
BWI76_RS11820: BWI76_RS11820 - short-chain dehydrogenase/reductase SDR, at 2,485,567 to 2,486,271
_RS11820
BWI76_RS11825: BWI76_RS11825 - LysR family transcriptional regulator, at 2,486,393 to 2,487,292
_RS11825
Position (kb)
2485
2486
2487 Strain fitness (log2 ratio)
-2
-1
0
1 at 2484.588 kb on + strand, within BWI76_RS11810 at 2484.801 kb on + strand, within BWI76_RS11810 at 2485.030 kb on + strand at 2485.279 kb on + strand, within BWI76_RS11815 at 2485.498 kb on - strand at 2486.168 kb on + strand, within BWI76_RS11820 at 2486.215 kb on - strand at 2486.317 kb on + strand at 2486.328 kb on + strand at 2486.423 kb on + strand at 2486.545 kb on + strand, within BWI76_RS11825 at 2486.553 kb on - strand, within BWI76_RS11825 at 2486.633 kb on + strand, within BWI76_RS11825 at 2486.977 kb on + strand, within BWI76_RS11825
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 2,484,588 + BWI76_RS11810 0.34 -0.8 2,484,801 + BWI76_RS11810 0.67 +0.1 2,485,030 + +1.1 2,485,279 + BWI76_RS11815 0.70 +1.1 2,485,498 - +0.1 2,486,168 + BWI76_RS11820 0.85 -1.7 2,486,215 - +0.3 2,486,317 + -1.9 2,486,328 + +0.2 2,486,423 + -0.0 2,486,545 + BWI76_RS11825 0.17 +0.2 2,486,553 - BWI76_RS11825 0.18 +1.6 2,486,633 + BWI76_RS11825 0.27 -0.4 2,486,977 + BWI76_RS11825 0.65 -0.4
Or see this region's nucleotide sequence