Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS10930

Experiment: RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS10925 and BWI76_RS10930 are separated by 252 nucleotidesBWI76_RS10930 and BWI76_RS10935 overlap by 8 nucleotides BWI76_RS10925: BWI76_RS10925 - glucans biosynthesis protein C, at 2,306,188 to 2,307,348 _RS10925 BWI76_RS10930: BWI76_RS10930 - glucan biosynthesis protein G, at 2,307,601 to 2,309,154 _RS10930 BWI76_RS10935: BWI76_RS10935 - glucan biosynthesis glucosyltransferase H, at 2,309,147 to 2,311,675 _RS10935 Position (kb) 2307 2308 2309 2310Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2 3at 2306.666 kb on - strand, within BWI76_RS10925at 2306.736 kb on - strand, within BWI76_RS10925at 2306.982 kb on + strand, within BWI76_RS10925at 2307.040 kb on + strand, within BWI76_RS10925at 2307.048 kb on - strand, within BWI76_RS10925at 2307.076 kb on + strand, within BWI76_RS10925at 2307.229 kb on + strand, within BWI76_RS10925at 2307.229 kb on + strandat 2307.229 kb on + strand, within BWI76_RS10925at 2307.440 kb on - strandat 2307.440 kb on - strandat 2307.462 kb on + strandat 2307.470 kb on - strandat 2307.483 kb on + strandat 2307.483 kb on + strandat 2307.491 kb on - strandat 2307.491 kb on - strandat 2307.492 kb on - strandat 2307.635 kb on + strandat 2307.635 kb on + strandat 2307.646 kb on + strandat 2307.654 kb on - strandat 2307.689 kb on + strandat 2307.734 kb on + strandat 2307.742 kb on - strandat 2307.759 kb on + strand, within BWI76_RS10930at 2307.767 kb on - strand, within BWI76_RS10930at 2307.767 kb on - strand, within BWI76_RS10930at 2307.782 kb on + strand, within BWI76_RS10930at 2307.978 kb on + strand, within BWI76_RS10930at 2307.991 kb on + strand, within BWI76_RS10930at 2307.991 kb on + strand, within BWI76_RS10930at 2307.991 kb on + strand, within BWI76_RS10930at 2307.991 kb on + strand, within BWI76_RS10930at 2307.997 kb on - strand, within BWI76_RS10930at 2307.999 kb on - strand, within BWI76_RS10930at 2308.325 kb on - strand, within BWI76_RS10930at 2308.504 kb on - strand, within BWI76_RS10930at 2308.544 kb on + strand, within BWI76_RS10930at 2308.633 kb on - strand, within BWI76_RS10930at 2308.882 kb on + strand, within BWI76_RS10930at 2309.377 kb on + strandat 2309.383 kb on - strandat 2309.655 kb on - strand, within BWI76_RS10935at 2309.778 kb on - strand, within BWI76_RS10935at 2309.847 kb on + strand, within BWI76_RS10935at 2310.070 kb on - strand, within BWI76_RS10935

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
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2,306,666 - BWI76_RS10925 0.41 +0.7
2,306,736 - BWI76_RS10925 0.47 -0.7
2,306,982 + BWI76_RS10925 0.68 -2.4
2,307,040 + BWI76_RS10925 0.73 -1.7
2,307,048 - BWI76_RS10925 0.74 -1.1
2,307,076 + BWI76_RS10925 0.76 -0.2
2,307,229 + BWI76_RS10925 0.90 -0.0
2,307,229 + -0.1
2,307,229 + BWI76_RS10925 0.90 +1.1
2,307,440 - +0.1
2,307,440 - -0.5
2,307,462 + +0.8
2,307,470 - -0.3
2,307,483 + +0.4
2,307,483 + +2.9
2,307,491 - -1.2
2,307,491 - -0.7
2,307,492 - -0.2
2,307,635 + -3.0
2,307,635 + -1.9
2,307,646 + +0.9
2,307,654 - -1.9
2,307,689 + -0.1
2,307,734 + -4.3
2,307,742 - +0.2
2,307,759 + BWI76_RS10930 0.10 -2.2
2,307,767 - BWI76_RS10930 0.11 -1.7
2,307,767 - BWI76_RS10930 0.11 -3.5
2,307,782 + BWI76_RS10930 0.12 -1.1
2,307,978 + BWI76_RS10930 0.24 -0.4
2,307,991 + BWI76_RS10930 0.25 -2.6
2,307,991 + BWI76_RS10930 0.25 -4.0
2,307,991 + BWI76_RS10930 0.25 -5.2
2,307,991 + BWI76_RS10930 0.25 -0.8
2,307,997 - BWI76_RS10930 0.25 -3.2
2,307,999 - BWI76_RS10930 0.26 -0.7
2,308,325 - BWI76_RS10930 0.47 -0.1
2,308,504 - BWI76_RS10930 0.58 -0.7
2,308,544 + BWI76_RS10930 0.61 -3.2
2,308,633 - BWI76_RS10930 0.66 +1.2
2,308,882 + BWI76_RS10930 0.82 -2.5
2,309,377 + -1.9
2,309,383 - -2.5
2,309,655 - BWI76_RS10935 0.20 -1.9
2,309,778 - BWI76_RS10935 0.25 -3.3
2,309,847 + BWI76_RS10935 0.28 -0.3
2,310,070 - BWI76_RS10935 0.36 +0.9

Or see this region's nucleotide sequence