Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS10745

Experiment: RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS10735 and BWI76_RS10740 are separated by 19 nucleotidesBWI76_RS10740 and BWI76_RS10745 are separated by 374 nucleotidesBWI76_RS10745 and BWI76_RS10750 are separated by 258 nucleotides BWI76_RS10735: BWI76_RS10735 - hypothetical protein, at 2,268,858 to 2,269,085 _RS10735 BWI76_RS10740: BWI76_RS10740 - NAD(P)H:quinone oxidoreductase, at 2,269,105 to 2,269,701 _RS10740 BWI76_RS10745: BWI76_RS10745 - hypothetical protein, at 2,270,076 to 2,270,249 _RS10745 BWI76_RS10750: BWI76_RS10750 - pyrimidine utilization transport protein G, at 2,270,508 to 2,271,830 _RS10750 Position (kb) 2270 2271Strain fitness (log2 ratio) -2 -1 0 1at 2269.145 kb on + strandat 2269.145 kb on + strandat 2269.610 kb on - strand, within BWI76_RS10740at 2269.634 kb on - strand, within BWI76_RS10740at 2269.652 kb on + strandat 2269.881 kb on - strandat 2269.988 kb on - strandat 2270.016 kb on + strandat 2270.024 kb on - strandat 2270.035 kb on + strandat 2270.159 kb on - strand, within BWI76_RS10745at 2270.275 kb on + strandat 2270.282 kb on - strandat 2270.551 kb on + strandat 2270.668 kb on + strand, within BWI76_RS10750at 2270.714 kb on - strand, within BWI76_RS10750at 2270.872 kb on - strand, within BWI76_RS10750at 2271.104 kb on - strand, within BWI76_RS10750

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
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2,269,145 + +0.2
2,269,145 + -0.3
2,269,610 - BWI76_RS10740 0.85 -1.0
2,269,634 - BWI76_RS10740 0.89 -0.4
2,269,652 + +1.0
2,269,881 - -0.7
2,269,988 - -0.6
2,270,016 + +0.2
2,270,024 - -0.2
2,270,035 + +0.7
2,270,159 - BWI76_RS10745 0.48 -2.7
2,270,275 + +0.2
2,270,282 - -1.8
2,270,551 + +0.2
2,270,668 + BWI76_RS10750 0.12 +1.2
2,270,714 - BWI76_RS10750 0.16 -0.6
2,270,872 - BWI76_RS10750 0.28 -0.0
2,271,104 - BWI76_RS10750 0.45 +1.2

Or see this region's nucleotide sequence