Experiment: RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10560 and BWI76_RS10565 are separated by 168 nucleotides BWI76_RS10565 and BWI76_RS10570 are separated by 214 nucleotides
BWI76_RS10560: BWI76_RS10560 - gamma-glutamyltransferase, at 2,230,064 to 2,231,647
_RS10560
BWI76_RS10565: BWI76_RS10565 - sugar isomerase, at 2,231,816 to 2,232,655
_RS10565
BWI76_RS10570: BWI76_RS10570 - cysteine ABC transporter substrate-binding protein, at 2,232,870 to 2,233,655
_RS10570
Position (kb)
2231
2232
2233 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2230.907 kb on - strand, within BWI76_RS10560 at 2230.999 kb on + strand, within BWI76_RS10560 at 2231.062 kb on + strand, within BWI76_RS10560 at 2231.079 kb on + strand, within BWI76_RS10560 at 2231.594 kb on + strand at 2231.926 kb on - strand, within BWI76_RS10565 at 2232.085 kb on - strand, within BWI76_RS10565 at 2232.153 kb on + strand, within BWI76_RS10565 at 2232.155 kb on - strand, within BWI76_RS10565 at 2232.228 kb on - strand, within BWI76_RS10565 at 2232.320 kb on + strand, within BWI76_RS10565 at 2232.893 kb on + strand at 2232.893 kb on + strand at 2233.032 kb on - strand, within BWI76_RS10570 at 2233.157 kb on - strand, within BWI76_RS10570 at 2233.221 kb on - strand, within BWI76_RS10570 at 2233.384 kb on - strand, within BWI76_RS10570
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 2,230,907 - BWI76_RS10560 0.53 +0.1 2,230,999 + BWI76_RS10560 0.59 +1.6 2,231,062 + BWI76_RS10560 0.63 +0.8 2,231,079 + BWI76_RS10560 0.64 +0.9 2,231,594 + -0.1 2,231,926 - BWI76_RS10565 0.13 +0.8 2,232,085 - BWI76_RS10565 0.32 -0.3 2,232,153 + BWI76_RS10565 0.40 +1.4 2,232,155 - BWI76_RS10565 0.40 +2.3 2,232,228 - BWI76_RS10565 0.49 +0.5 2,232,320 + BWI76_RS10565 0.60 +0.8 2,232,893 + +0.2 2,232,893 + +1.7 2,233,032 - BWI76_RS10570 0.21 +0.5 2,233,157 - BWI76_RS10570 0.37 -0.3 2,233,221 - BWI76_RS10570 0.45 -2.0 2,233,384 - BWI76_RS10570 0.65 +0.5
Or see this region's nucleotide sequence