Experiment: RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09645 and BWI76_RS09650 are separated by 403 nucleotides BWI76_RS09650 and BWI76_RS09655 overlap by 72 nucleotides BWI76_RS09655 and BWI76_RS09660 are separated by 135 nucleotides
BWI76_RS09645: BWI76_RS09645 - thioredoxin-disulfide reductase, at 2,018,776 to 2,019,744
_RS09645
BWI76_RS09650: BWI76_RS09650 - hypothetical protein, at 2,020,148 to 2,020,347
_RS09650
BWI76_RS09655: BWI76_RS09655 - leucine-responsive regulatory protein, at 2,020,276 to 2,020,770
_RS09655
BWI76_RS09660: BWI76_RS09660 - DNA translocase FtsK, at 2,020,906 to 2,025,024
_RS09660
Position (kb)
2020
2021 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2019.640 kb on + strand, within BWI76_RS09645 at 2019.688 kb on + strand at 2019.943 kb on + strand at 2020.109 kb on + strand at 2020.122 kb on - strand at 2020.168 kb on - strand, within BWI76_RS09650 at 2020.171 kb on + strand, within BWI76_RS09650 at 2020.171 kb on + strand, within BWI76_RS09650 at 2020.179 kb on - strand, within BWI76_RS09650 at 2020.189 kb on - strand, within BWI76_RS09650 at 2020.204 kb on - strand, within BWI76_RS09650 at 2020.204 kb on - strand, within BWI76_RS09650 at 2020.204 kb on - strand, within BWI76_RS09650 at 2020.211 kb on + strand, within BWI76_RS09650 at 2020.243 kb on + strand, within BWI76_RS09650 at 2020.254 kb on + strand, within BWI76_RS09650 at 2020.408 kb on + strand, within BWI76_RS09655 at 2020.452 kb on + strand, within BWI76_RS09655 at 2020.460 kb on - strand, within BWI76_RS09655 at 2020.465 kb on - strand, within BWI76_RS09655 at 2020.481 kb on + strand, within BWI76_RS09655 at 2020.707 kb on - strand, within BWI76_RS09655
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 2,019,640 + BWI76_RS09645 0.89 -2.0 2,019,688 + -1.8 2,019,943 + -0.6 2,020,109 + -0.4 2,020,122 - -0.6 2,020,168 - BWI76_RS09650 0.10 +0.4 2,020,171 + BWI76_RS09650 0.12 -0.4 2,020,171 + BWI76_RS09650 0.12 -0.5 2,020,179 - BWI76_RS09650 0.15 -1.0 2,020,189 - BWI76_RS09650 0.20 -2.2 2,020,204 - BWI76_RS09650 0.28 -2.5 2,020,204 - BWI76_RS09650 0.28 +1.0 2,020,204 - BWI76_RS09650 0.28 -2.6 2,020,211 + BWI76_RS09650 0.32 -1.6 2,020,243 + BWI76_RS09650 0.47 -0.3 2,020,254 + BWI76_RS09650 0.53 -2.5 2,020,408 + BWI76_RS09655 0.27 -1.6 2,020,452 + BWI76_RS09655 0.36 -2.4 2,020,460 - BWI76_RS09655 0.37 -0.3 2,020,465 - BWI76_RS09655 0.38 -0.6 2,020,481 + BWI76_RS09655 0.41 -2.9 2,020,707 - BWI76_RS09655 0.87 -0.9
Or see this region's nucleotide sequence