Experiment: RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08495 and BWI76_RS08500 are separated by 15 nucleotides BWI76_RS08500 and BWI76_RS08505 are separated by 15 nucleotides BWI76_RS08505 and BWI76_RS08510 overlap by 1 nucleotides BWI76_RS08510 and BWI76_RS08515 are separated by 149 nucleotides BWI76_RS08515 and BWI76_RS08520 are separated by 5 nucleotides
BWI76_RS08495: BWI76_RS08495 - cytochrome bd-I ubiquinol oxidase subunit I, at 1,800,095 to 1,801,663
_RS08495
BWI76_RS08500: BWI76_RS08500 - cytochrome d ubiquinol oxidase subunit 2, at 1,801,679 to 1,802,818
_RS08500
BWI76_RS08505: BWI76_RS08505 - cytochrome bd-I oxidase subunit CydX, at 1,802,834 to 1,802,947
_RS08505
BWI76_RS08510: BWI76_RS08510 - cyd operon protein YbgE, at 1,802,947 to 1,803,237
_RS08510
BWI76_RS08515: BWI76_RS08515 - acyl-CoA thioesterase YbgC, at 1,803,387 to 1,803,791
_RS08515
BWI76_RS08520: BWI76_RS08520 - colicin transporter TolQ, at 1,803,797 to 1,804,480
_RS08520
Position (kb) 1801
1802
1803 Strain fitness (log2 ratio) -2
-1
0
1 at 1801.011 kb on + strand, within BWI76_RS08495 at 1801.181 kb on - strand, within BWI76_RS08495 at 1801.313 kb on + strand, within BWI76_RS08495 at 1801.380 kb on - strand, within BWI76_RS08495 at 1801.545 kb on - strand at 1801.909 kb on + strand, within BWI76_RS08500 at 1801.917 kb on - strand, within BWI76_RS08500 at 1802.639 kb on + strand, within BWI76_RS08500 at 1802.734 kb on + strand at 1802.812 kb on + strand at 1802.965 kb on + strand at 1803.068 kb on + strand, within BWI76_RS08510 at 1803.086 kb on - strand, within BWI76_RS08510 at 1803.105 kb on + strand, within BWI76_RS08510 at 1803.125 kb on + strand, within BWI76_RS08510 at 1803.447 kb on + strand, within BWI76_RS08515 at 1803.764 kb on + strand at 1803.812 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 1,801,011 + BWI76_RS08495 0.58 +1.0 1,801,181 - BWI76_RS08495 0.69 -0.6 1,801,313 + BWI76_RS08495 0.78 -1.1 1,801,380 - BWI76_RS08495 0.82 -0.0 1,801,545 - -1.6 1,801,909 + BWI76_RS08500 0.20 -1.0 1,801,917 - BWI76_RS08500 0.21 -1.1 1,802,639 + BWI76_RS08500 0.84 -1.3 1,802,734 + -0.0 1,802,812 + +1.7 1,802,965 + +0.3 1,803,068 + BWI76_RS08510 0.42 +0.3 1,803,086 - BWI76_RS08510 0.48 +0.8 1,803,105 + BWI76_RS08510 0.54 +0.1 1,803,125 + BWI76_RS08510 0.61 +0.1 1,803,447 + BWI76_RS08515 0.15 +0.4 1,803,764 + +0.5 1,803,812 - -2.3
Or see this region's nucleotide sequence