Experiment: RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08200 and BWI76_RS08205 are separated by 45 nucleotides BWI76_RS08205 and BWI76_RS08210 are separated by 8 nucleotides
BWI76_RS08200: BWI76_RS08200 - UMP phosphatase, at 1,734,685 to 1,735,437
_RS08200
BWI76_RS08205: BWI76_RS08205 - DNA-binding transcriptional dual regulator NagC, at 1,735,483 to 1,736,703
_RS08205
BWI76_RS08210: BWI76_RS08210 - N-acetylglucosamine-6-phosphate deacetylase, at 1,736,712 to 1,737,860
_RS08210
Position (kb)
1735
1736
1737 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1734.625 kb on + strand at 1734.655 kb on + strand at 1734.659 kb on + strand at 1734.659 kb on + strand at 1734.659 kb on + strand at 1734.659 kb on + strand at 1734.659 kb on + strand at 1734.659 kb on + strand at 1734.664 kb on + strand at 1734.667 kb on - strand at 1734.667 kb on - strand at 1734.667 kb on - strand at 1734.667 kb on - strand at 1734.667 kb on - strand at 1734.669 kb on + strand at 1734.669 kb on + strand at 1734.672 kb on - strand at 1734.672 kb on - strand at 1734.674 kb on + strand at 1734.677 kb on - strand at 1734.677 kb on - strand at 1734.677 kb on - strand at 1734.679 kb on - strand at 1734.679 kb on - strand at 1734.679 kb on - strand at 1734.728 kb on + strand at 1734.728 kb on + strand at 1734.750 kb on - strand at 1734.753 kb on - strand at 1734.775 kb on - strand, within BWI76_RS08200 at 1734.790 kb on + strand, within BWI76_RS08200 at 1734.790 kb on + strand, within BWI76_RS08200 at 1734.809 kb on - strand, within BWI76_RS08200 at 1734.831 kb on + strand, within BWI76_RS08200 at 1734.855 kb on + strand, within BWI76_RS08200 at 1734.856 kb on - strand, within BWI76_RS08200 at 1734.863 kb on - strand, within BWI76_RS08200 at 1734.863 kb on - strand, within BWI76_RS08200 at 1734.864 kb on - strand, within BWI76_RS08200 at 1735.133 kb on + strand, within BWI76_RS08200 at 1735.141 kb on - strand, within BWI76_RS08200 at 1735.236 kb on - strand, within BWI76_RS08200 at 1735.312 kb on - strand, within BWI76_RS08200 at 1735.433 kb on + strand at 1735.441 kb on - strand at 1735.514 kb on + strand at 1735.730 kb on + strand, within BWI76_RS08205 at 1735.915 kb on + strand, within BWI76_RS08205 at 1736.060 kb on - strand, within BWI76_RS08205 at 1736.300 kb on - strand, within BWI76_RS08205 at 1736.394 kb on - strand, within BWI76_RS08205 at 1736.430 kb on - strand, within BWI76_RS08205 at 1737.060 kb on - strand, within BWI76_RS08210 at 1737.418 kb on - strand, within BWI76_RS08210
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 1,734,625 + +0.6 1,734,655 + +1.0 1,734,659 + -1.3 1,734,659 + +0.6 1,734,659 + -3.8 1,734,659 + -0.0 1,734,659 + -1.0 1,734,659 + -0.1 1,734,664 + +1.0 1,734,667 - -2.0 1,734,667 - +0.1 1,734,667 - +0.5 1,734,667 - -0.1 1,734,667 - +0.5 1,734,669 + +0.2 1,734,669 + +0.1 1,734,672 - +1.2 1,734,672 - +1.0 1,734,674 + -0.3 1,734,677 - -1.8 1,734,677 - +0.1 1,734,677 - -1.9 1,734,679 - +0.3 1,734,679 - +1.2 1,734,679 - -0.3 1,734,728 + +0.9 1,734,728 + +1.3 1,734,750 - +0.4 1,734,753 - -0.0 1,734,775 - BWI76_RS08200 0.12 -1.0 1,734,790 + BWI76_RS08200 0.14 -1.1 1,734,790 + BWI76_RS08200 0.14 -1.0 1,734,809 - BWI76_RS08200 0.16 -0.3 1,734,831 + BWI76_RS08200 0.19 -3.1 1,734,855 + BWI76_RS08200 0.23 -2.3 1,734,856 - BWI76_RS08200 0.23 -0.3 1,734,863 - BWI76_RS08200 0.24 +0.2 1,734,863 - BWI76_RS08200 0.24 +0.2 1,734,864 - BWI76_RS08200 0.24 -2.5 1,735,133 + BWI76_RS08200 0.59 +0.5 1,735,141 - BWI76_RS08200 0.61 +0.1 1,735,236 - BWI76_RS08200 0.73 -0.5 1,735,312 - BWI76_RS08200 0.83 +2.0 1,735,433 + +1.0 1,735,441 - -1.0 1,735,514 + +1.0 1,735,730 + BWI76_RS08205 0.20 +1.0 1,735,915 + BWI76_RS08205 0.35 -0.2 1,736,060 - BWI76_RS08205 0.47 -2.7 1,736,300 - BWI76_RS08205 0.67 -1.5 1,736,394 - BWI76_RS08205 0.75 -1.2 1,736,430 - BWI76_RS08205 0.78 -2.7 1,737,060 - BWI76_RS08210 0.30 -2.7 1,737,418 - BWI76_RS08210 0.61 +1.2
Or see this region's nucleotide sequence