Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS08205

Experiment: RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS08200 and BWI76_RS08205 are separated by 45 nucleotidesBWI76_RS08205 and BWI76_RS08210 are separated by 8 nucleotides BWI76_RS08200: BWI76_RS08200 - UMP phosphatase, at 1,734,685 to 1,735,437 _RS08200 BWI76_RS08205: BWI76_RS08205 - DNA-binding transcriptional dual regulator NagC, at 1,735,483 to 1,736,703 _RS08205 BWI76_RS08210: BWI76_RS08210 - N-acetylglucosamine-6-phosphate deacetylase, at 1,736,712 to 1,737,860 _RS08210 Position (kb) 1735 1736 1737Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 1734.625 kb on + strandat 1734.655 kb on + strandat 1734.659 kb on + strandat 1734.659 kb on + strandat 1734.659 kb on + strandat 1734.659 kb on + strandat 1734.659 kb on + strandat 1734.659 kb on + strandat 1734.664 kb on + strandat 1734.667 kb on - strandat 1734.667 kb on - strandat 1734.667 kb on - strandat 1734.667 kb on - strandat 1734.667 kb on - strandat 1734.669 kb on + strandat 1734.669 kb on + strandat 1734.672 kb on - strandat 1734.672 kb on - strandat 1734.674 kb on + strandat 1734.677 kb on - strandat 1734.677 kb on - strandat 1734.677 kb on - strandat 1734.679 kb on - strandat 1734.679 kb on - strandat 1734.679 kb on - strandat 1734.728 kb on + strandat 1734.728 kb on + strandat 1734.750 kb on - strandat 1734.753 kb on - strandat 1734.775 kb on - strand, within BWI76_RS08200at 1734.790 kb on + strand, within BWI76_RS08200at 1734.790 kb on + strand, within BWI76_RS08200at 1734.809 kb on - strand, within BWI76_RS08200at 1734.831 kb on + strand, within BWI76_RS08200at 1734.855 kb on + strand, within BWI76_RS08200at 1734.856 kb on - strand, within BWI76_RS08200at 1734.863 kb on - strand, within BWI76_RS08200at 1734.863 kb on - strand, within BWI76_RS08200at 1734.864 kb on - strand, within BWI76_RS08200at 1735.133 kb on + strand, within BWI76_RS08200at 1735.141 kb on - strand, within BWI76_RS08200at 1735.236 kb on - strand, within BWI76_RS08200at 1735.312 kb on - strand, within BWI76_RS08200at 1735.433 kb on + strandat 1735.441 kb on - strandat 1735.514 kb on + strandat 1735.730 kb on + strand, within BWI76_RS08205at 1735.915 kb on + strand, within BWI76_RS08205at 1736.060 kb on - strand, within BWI76_RS08205at 1736.300 kb on - strand, within BWI76_RS08205at 1736.394 kb on - strand, within BWI76_RS08205at 1736.430 kb on - strand, within BWI76_RS08205at 1737.060 kb on - strand, within BWI76_RS08210at 1737.418 kb on - strand, within BWI76_RS08210

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
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1,734,625 + +0.6
1,734,655 + +1.0
1,734,659 + -1.3
1,734,659 + +0.6
1,734,659 + -3.8
1,734,659 + -0.0
1,734,659 + -1.0
1,734,659 + -0.1
1,734,664 + +1.0
1,734,667 - -2.0
1,734,667 - +0.1
1,734,667 - +0.5
1,734,667 - -0.1
1,734,667 - +0.5
1,734,669 + +0.2
1,734,669 + +0.1
1,734,672 - +1.2
1,734,672 - +1.0
1,734,674 + -0.3
1,734,677 - -1.8
1,734,677 - +0.1
1,734,677 - -1.9
1,734,679 - +0.3
1,734,679 - +1.2
1,734,679 - -0.3
1,734,728 + +0.9
1,734,728 + +1.3
1,734,750 - +0.4
1,734,753 - -0.0
1,734,775 - BWI76_RS08200 0.12 -1.0
1,734,790 + BWI76_RS08200 0.14 -1.1
1,734,790 + BWI76_RS08200 0.14 -1.0
1,734,809 - BWI76_RS08200 0.16 -0.3
1,734,831 + BWI76_RS08200 0.19 -3.1
1,734,855 + BWI76_RS08200 0.23 -2.3
1,734,856 - BWI76_RS08200 0.23 -0.3
1,734,863 - BWI76_RS08200 0.24 +0.2
1,734,863 - BWI76_RS08200 0.24 +0.2
1,734,864 - BWI76_RS08200 0.24 -2.5
1,735,133 + BWI76_RS08200 0.59 +0.5
1,735,141 - BWI76_RS08200 0.61 +0.1
1,735,236 - BWI76_RS08200 0.73 -0.5
1,735,312 - BWI76_RS08200 0.83 +2.0
1,735,433 + +1.0
1,735,441 - -1.0
1,735,514 + +1.0
1,735,730 + BWI76_RS08205 0.20 +1.0
1,735,915 + BWI76_RS08205 0.35 -0.2
1,736,060 - BWI76_RS08205 0.47 -2.7
1,736,300 - BWI76_RS08205 0.67 -1.5
1,736,394 - BWI76_RS08205 0.75 -1.2
1,736,430 - BWI76_RS08205 0.78 -2.7
1,737,060 - BWI76_RS08210 0.30 -2.7
1,737,418 - BWI76_RS08210 0.61 +1.2

Or see this region's nucleotide sequence