Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS08020

Experiment: RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
1,699,151	+				+0.9
1,699,755	-		BWI76_RS08020	0.15	-4.0
1,700,122	-		BWI76_RS08020	0.46	-0.6
1,700,205	+		BWI76_RS08020	0.53	-1.4
1,700,215	+		BWI76_RS08020	0.54	+2.0
1,700,223	-		BWI76_RS08020	0.54	-4.7
1,700,259	-		BWI76_RS08020	0.57	-2.5
1,700,467	+		BWI76_RS08020	0.75	-1.0
1,700,504	+		BWI76_RS08020	0.78	-1.3
1,700,504	+		BWI76_RS08020	0.78	-2.7
1,700,707	+				-4.6
1,700,941	+				-0.7
1,700,949	-				-1.2
1,701,202	+		BWI76_RS08025	0.26	+0.7
1,701,311	-		BWI76_RS08025	0.36	-1.1
1,701,437	+		BWI76_RS08025	0.48	-0.8
1,701,525	+		BWI76_RS08025	0.55	+0.2
1,701,700	-		BWI76_RS08025	0.71	+0.6