Experiment: RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS07455 and BWI76_RS07460 are separated by 118 nucleotides BWI76_RS07460 and BWI76_RS07465 overlap by 1 nucleotides BWI76_RS07465 and BWI76_RS07470 are separated by 190 nucleotides
BWI76_RS07455: BWI76_RS07455 - NAD(P)H nitroreductase, at 1,573,790 to 1,574,443
_RS07455
BWI76_RS07460: BWI76_RS07460 - hypothetical protein, at 1,574,562 to 1,574,930
_RS07460
BWI76_RS07465: BWI76_RS07465 - TetR family transcriptional regulator, at 1,574,930 to 1,575,511
_RS07465
BWI76_RS07470: BWI76_RS07470 - MBL fold metallo-hydrolase, at 1,575,702 to 1,576,802
_RS07470
Position (kb) 1574
1575
1576 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1574.160 kb on + strand, within BWI76_RS07455 at 1574.165 kb on + strand, within BWI76_RS07455 at 1574.465 kb on - strand at 1574.702 kb on + strand, within BWI76_RS07460 at 1574.900 kb on + strand at 1574.947 kb on - strand at 1575.250 kb on - strand, within BWI76_RS07465 at 1575.306 kb on + strand, within BWI76_RS07465 at 1575.514 kb on - strand at 1575.575 kb on - strand at 1575.835 kb on - strand, within BWI76_RS07470 at 1575.968 kb on + strand, within BWI76_RS07470 at 1576.364 kb on + strand, within BWI76_RS07470
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 1,574,160 + BWI76_RS07455 0.57 +0.4 1,574,165 + BWI76_RS07455 0.57 -1.3 1,574,465 - -0.4 1,574,702 + BWI76_RS07460 0.38 -0.2 1,574,900 + +1.0 1,574,947 - -1.0 1,575,250 - BWI76_RS07465 0.55 -3.1 1,575,306 + BWI76_RS07465 0.65 -1.6 1,575,514 - -0.0 1,575,575 - -2.2 1,575,835 - BWI76_RS07470 0.12 +0.1 1,575,968 + BWI76_RS07470 0.24 -0.6 1,576,364 + BWI76_RS07470 0.60 -0.3
Or see this region's nucleotide sequence