Experiment: RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS07065 and BWI76_RS07070 overlap by 4 nucleotides BWI76_RS07070 and BWI76_RS07075 are separated by 96 nucleotides BWI76_RS07075 and BWI76_RS07080 are separated by 106 nucleotides
BWI76_RS07065: BWI76_RS07065 - 5-(carboxyamino)imidazole ribonucleotide synthase, at 1,486,550 to 1,487,617
_RS07065
BWI76_RS07070: BWI76_RS07070 - 5-(carboxyamino)imidazole ribonucleotide mutase, at 1,487,614 to 1,488,123
_RS07070
BWI76_RS07075: BWI76_RS07075 - hypothetical protein, at 1,488,220 to 1,488,549
_RS07075
BWI76_RS07080: BWI76_RS07080 - UDP-2,3-diacylglucosamine diphosphatase, at 1,488,656 to 1,489,378
_RS07080
Position (kb)
1487
1488
1489 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1486.753 kb on - strand, within BWI76_RS07065 at 1486.967 kb on - strand, within BWI76_RS07065 at 1487.019 kb on + strand, within BWI76_RS07065 at 1487.154 kb on - strand, within BWI76_RS07065 at 1487.222 kb on + strand, within BWI76_RS07065 at 1487.293 kb on - strand, within BWI76_RS07065 at 1487.293 kb on - strand, within BWI76_RS07065 at 1487.639 kb on - strand at 1487.641 kb on + strand at 1487.694 kb on + strand, within BWI76_RS07070 at 1487.835 kb on - strand, within BWI76_RS07070 at 1487.847 kb on - strand, within BWI76_RS07070 at 1487.901 kb on - strand, within BWI76_RS07070 at 1487.962 kb on - strand, within BWI76_RS07070 at 1488.071 kb on + strand, within BWI76_RS07070 at 1488.148 kb on + strand at 1488.156 kb on - strand at 1488.158 kb on + strand at 1488.158 kb on + strand at 1488.344 kb on + strand, within BWI76_RS07075 at 1488.344 kb on + strand, within BWI76_RS07075 at 1488.375 kb on + strand, within BWI76_RS07075 at 1488.435 kb on + strand, within BWI76_RS07075
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 1,486,753 - BWI76_RS07065 0.19 -4.4 1,486,967 - BWI76_RS07065 0.39 -4.5 1,487,019 + BWI76_RS07065 0.44 +1.0 1,487,154 - BWI76_RS07065 0.57 -3.7 1,487,222 + BWI76_RS07065 0.63 -4.9 1,487,293 - BWI76_RS07065 0.70 -4.1 1,487,293 - BWI76_RS07065 0.70 -4.2 1,487,639 - -1.2 1,487,641 + -2.6 1,487,694 + BWI76_RS07070 0.16 -5.0 1,487,835 - BWI76_RS07070 0.43 -1.8 1,487,847 - BWI76_RS07070 0.46 -4.5 1,487,901 - BWI76_RS07070 0.56 -3.7 1,487,962 - BWI76_RS07070 0.68 +1.0 1,488,071 + BWI76_RS07070 0.90 -1.8 1,488,148 + +0.0 1,488,156 - -5.5 1,488,158 + -2.7 1,488,158 + -2.7 1,488,344 + BWI76_RS07075 0.38 +0.8 1,488,344 + BWI76_RS07075 0.38 -1.3 1,488,375 + BWI76_RS07075 0.47 -2.7 1,488,435 + BWI76_RS07075 0.65 -0.0
Or see this region's nucleotide sequence