Experiment: RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05785 and BWI76_RS05790 overlap by 4 nucleotides BWI76_RS05790 and BWI76_RS05795 are separated by 551 nucleotides BWI76_RS05795 and BWI76_RS05800 overlap by 4 nucleotides BWI76_RS05800 and BWI76_RS05805 are separated by 17 nucleotides BWI76_RS05805 and BWI76_RS05810 overlap by 37 nucleotides
BWI76_RS05785: BWI76_RS05785 - CI repressor, at 1,217,449 to 1,218,000
_RS05785
BWI76_RS05790: BWI76_RS05790 - phage DNA-binding protein, at 1,217,997 to 1,218,263
_RS05790
BWI76_RS05795: BWI76_RS05795 - hypothetical protein, at 1,218,815 to 1,219,552
_RS05795
BWI76_RS05800: BWI76_RS05800 - phage transcriptional activator, Ogr/delta, at 1,219,549 to 1,219,794
_RS05800
BWI76_RS05805: BWI76_RS05805 - phage polarity suppression protein, at 1,219,812 to 1,220,378
_RS05805
BWI76_RS05810: BWI76_RS05810 - hypothetical protein, at 1,220,342 to 1,220,527
_RS05810
Position (kb) 1218
1219
1220 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1218.149 kb on + strand, within BWI76_RS05790 at 1218.309 kb on + strand at 1218.354 kb on - strand at 1218.422 kb on - strand at 1218.422 kb on - strand at 1218.756 kb on + strand at 1218.859 kb on + strand at 1218.920 kb on - strand, within BWI76_RS05795 at 1218.934 kb on - strand, within BWI76_RS05795 at 1219.129 kb on + strand, within BWI76_RS05795 at 1219.137 kb on - strand at 1219.192 kb on - strand, within BWI76_RS05795 at 1219.233 kb on - strand, within BWI76_RS05795 at 1219.320 kb on - strand, within BWI76_RS05795 at 1219.380 kb on + strand, within BWI76_RS05795 at 1219.532 kb on - strand at 1219.752 kb on + strand, within BWI76_RS05800 at 1219.947 kb on - strand, within BWI76_RS05805 at 1219.965 kb on - strand, within BWI76_RS05805 at 1219.980 kb on - strand, within BWI76_RS05805 at 1219.996 kb on + strand, within BWI76_RS05805 at 1220.092 kb on + strand, within BWI76_RS05805 at 1220.105 kb on - strand, within BWI76_RS05805 at 1220.161 kb on + strand, within BWI76_RS05805 at 1220.166 kb on + strand, within BWI76_RS05805 at 1220.166 kb on + strand, within BWI76_RS05805 at 1220.166 kb on + strand, within BWI76_RS05805 at 1220.213 kb on + strand, within BWI76_RS05805 at 1220.221 kb on - strand, within BWI76_RS05805 at 1220.361 kb on + strand at 1220.432 kb on - strand, within BWI76_RS05810
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 1,218,149 + BWI76_RS05790 0.57 +0.2 1,218,309 + +0.1 1,218,354 - +0.0 1,218,422 - -2.0 1,218,422 - -3.1 1,218,756 + -0.7 1,218,859 + -0.5 1,218,920 - BWI76_RS05795 0.14 +0.0 1,218,934 - BWI76_RS05795 0.16 -0.2 1,219,129 + BWI76_RS05795 0.43 -1.9 1,219,137 - +1.0 1,219,192 - BWI76_RS05795 0.51 -2.1 1,219,233 - BWI76_RS05795 0.57 +0.1 1,219,320 - BWI76_RS05795 0.68 -1.1 1,219,380 + BWI76_RS05795 0.77 +2.6 1,219,532 - +0.5 1,219,752 + BWI76_RS05800 0.83 +1.0 1,219,947 - BWI76_RS05805 0.24 -1.8 1,219,965 - BWI76_RS05805 0.27 -0.3 1,219,980 - BWI76_RS05805 0.30 -1.0 1,219,996 + BWI76_RS05805 0.32 +1.1 1,220,092 + BWI76_RS05805 0.49 -0.3 1,220,105 - BWI76_RS05805 0.52 +1.4 1,220,161 + BWI76_RS05805 0.62 -0.3 1,220,166 + BWI76_RS05805 0.62 -1.5 1,220,166 + BWI76_RS05805 0.62 -0.4 1,220,166 + BWI76_RS05805 0.62 -2.3 1,220,213 + BWI76_RS05805 0.71 -0.1 1,220,221 - BWI76_RS05805 0.72 -0.9 1,220,361 + -0.6 1,220,432 - BWI76_RS05810 0.48 +0.4
Or see this region's nucleotide sequence