Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS05515

Experiment: RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS05505 and BWI76_RS05510 are separated by 6 nucleotidesBWI76_RS05510 and BWI76_RS05515 are separated by 11 nucleotidesBWI76_RS05515 and BWI76_RS05520 are separated by 2 nucleotidesBWI76_RS05520 and BWI76_RS05525 are separated by 32 nucleotides BWI76_RS05505: BWI76_RS05505 - NADH:ubiquinone reductase (Na(+)-transporting) subunit D, at 1,155,199 to 1,155,837 _RS05505 BWI76_RS05510: BWI76_RS05510 - NADH:ubiquinone reductase (Na(+)-transporting) subunit E, at 1,155,844 to 1,156,440 _RS05510 BWI76_RS05515: BWI76_RS05515 - NADH:ubiquinone reductase (Na(+)-transporting) subunit F, at 1,156,452 to 1,157,675 _RS05515 BWI76_RS05520: BWI76_RS05520 - hypothetical protein, at 1,157,678 to 1,157,893 _RS05520 BWI76_RS05525: BWI76_RS05525 - glycerophosphodiester phosphodiesterase, at 1,157,926 to 1,158,783 _RS05525 Position (kb) 1156 1157 1158Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 1155.862 kb on - strandat 1156.033 kb on - strand, within BWI76_RS05510at 1156.158 kb on + strand, within BWI76_RS05510at 1156.158 kb on + strand, within BWI76_RS05510at 1156.166 kb on - strand, within BWI76_RS05510at 1156.375 kb on - strand, within BWI76_RS05510at 1156.375 kb on - strand, within BWI76_RS05510at 1156.511 kb on + strandat 1156.733 kb on + strand, within BWI76_RS05515at 1156.858 kb on - strand, within BWI76_RS05515at 1157.126 kb on - strand, within BWI76_RS05515at 1157.340 kb on + strand, within BWI76_RS05515at 1157.773 kb on - strand, within BWI76_RS05520at 1157.809 kb on + strand, within BWI76_RS05520at 1157.820 kb on + strand, within BWI76_RS05520at 1157.820 kb on + strand, within BWI76_RS05520at 1157.828 kb on - strand, within BWI76_RS05520at 1158.058 kb on + strand, within BWI76_RS05525at 1158.058 kb on + strand, within BWI76_RS05525at 1158.329 kb on - strand, within BWI76_RS05525at 1158.330 kb on - strand, within BWI76_RS05525at 1158.542 kb on - strand, within BWI76_RS05525

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
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1,155,862 - -1.7
1,156,033 - BWI76_RS05510 0.32 +0.2
1,156,158 + BWI76_RS05510 0.53 +0.4
1,156,158 + BWI76_RS05510 0.53 -2.7
1,156,166 - BWI76_RS05510 0.54 -0.8
1,156,375 - BWI76_RS05510 0.89 +1.6
1,156,375 - BWI76_RS05510 0.89 +0.1
1,156,511 + -0.2
1,156,733 + BWI76_RS05515 0.23 -4.0
1,156,858 - BWI76_RS05515 0.33 -0.5
1,157,126 - BWI76_RS05515 0.55 -3.6
1,157,340 + BWI76_RS05515 0.73 -2.2
1,157,773 - BWI76_RS05520 0.44 -1.9
1,157,809 + BWI76_RS05520 0.61 -0.2
1,157,820 + BWI76_RS05520 0.66 -1.2
1,157,820 + BWI76_RS05520 0.66 -1.3
1,157,828 - BWI76_RS05520 0.69 +1.1
1,158,058 + BWI76_RS05525 0.15 -0.4
1,158,058 + BWI76_RS05525 0.15 -0.1
1,158,329 - BWI76_RS05525 0.47 +0.7
1,158,330 - BWI76_RS05525 0.47 -0.4
1,158,542 - BWI76_RS05525 0.72 +0.1

Or see this region's nucleotide sequence