Experiment: RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04675 and BWI76_RS04680 overlap by 1 nucleotides BWI76_RS04680 and BWI76_RS04685 are separated by 95 nucleotides BWI76_RS04685 and BWI76_RS04690 are separated by 621 nucleotides
BWI76_RS04675: BWI76_RS04675 - 3-isopropylmalate dehydrogenase, at 975,088 to 976,179
_RS04675
BWI76_RS04680: BWI76_RS04680 - 2-isopropylmalate synthase, at 976,179 to 977,750
_RS04680
BWI76_RS04685: BWI76_RS04685 - putative leucine operon leader peptide, at 977,846 to 977,932
_RS04685
BWI76_RS04690: BWI76_RS04690 - transcriptional regulator LeuO, at 978,554 to 979,504
_RS04690
Position (kb) 976
977
978 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 975.438 kb on + strand, within BWI76_RS04675 at 975.473 kb on + strand, within BWI76_RS04675 at 975.788 kb on - strand, within BWI76_RS04675 at 975.833 kb on - strand, within BWI76_RS04675 at 975.841 kb on - strand, within BWI76_RS04675 at 975.899 kb on + strand, within BWI76_RS04675 at 975.900 kb on - strand, within BWI76_RS04675 at 976.189 kb on + strand at 976.321 kb on - strand at 976.321 kb on - strand at 976.368 kb on - strand, within BWI76_RS04680 at 976.375 kb on + strand, within BWI76_RS04680 at 976.383 kb on - strand, within BWI76_RS04680 at 976.388 kb on - strand, within BWI76_RS04680 at 976.423 kb on - strand, within BWI76_RS04680 at 976.517 kb on + strand, within BWI76_RS04680 at 976.628 kb on + strand, within BWI76_RS04680 at 976.696 kb on - strand, within BWI76_RS04680 at 976.775 kb on + strand, within BWI76_RS04680 at 976.807 kb on - strand, within BWI76_RS04680 at 976.858 kb on + strand, within BWI76_RS04680 at 976.895 kb on - strand, within BWI76_RS04680 at 976.918 kb on + strand, within BWI76_RS04680 at 977.079 kb on - strand, within BWI76_RS04680 at 977.084 kb on - strand, within BWI76_RS04680 at 977.091 kb on + strand, within BWI76_RS04680 at 977.104 kb on - strand, within BWI76_RS04680 at 977.135 kb on - strand, within BWI76_RS04680 at 977.172 kb on + strand, within BWI76_RS04680 at 977.222 kb on + strand, within BWI76_RS04680 at 977.222 kb on + strand, within BWI76_RS04680 at 977.234 kb on - strand, within BWI76_RS04680 at 977.361 kb on - strand, within BWI76_RS04680 at 977.361 kb on - strand, within BWI76_RS04680 at 977.450 kb on + strand, within BWI76_RS04680 at 977.550 kb on + strand, within BWI76_RS04680 at 977.550 kb on + strand, within BWI76_RS04680 at 977.571 kb on + strand, within BWI76_RS04680 at 977.572 kb on - strand, within BWI76_RS04680 at 977.579 kb on - strand, within BWI76_RS04680 at 977.627 kb on + strand at 977.627 kb on + strand at 977.632 kb on + strand at 977.686 kb on - strand at 977.688 kb on + strand at 977.688 kb on + strand at 977.702 kb on + strand at 977.707 kb on - strand at 977.707 kb on - strand at 977.710 kb on - strand at 977.712 kb on + strand at 977.712 kb on + strand at 977.720 kb on - strand at 977.720 kb on - strand at 977.760 kb on - strand at 977.760 kb on - strand at 977.769 kb on + strand at 977.771 kb on + strand at 977.777 kb on - strand at 977.781 kb on - strand at 977.820 kb on + strand at 977.844 kb on + strand at 977.859 kb on + strand, within BWI76_RS04685 at 977.866 kb on + strand, within BWI76_RS04685 at 977.866 kb on + strand, within BWI76_RS04685 at 977.874 kb on - strand, within BWI76_RS04685 at 977.899 kb on + strand, within BWI76_RS04685 at 977.899 kb on + strand, within BWI76_RS04685 at 977.899 kb on + strand, within BWI76_RS04685 at 977.899 kb on + strand, within BWI76_RS04685 at 977.899 kb on - strand, within BWI76_RS04685 at 977.899 kb on - strand, within BWI76_RS04685 at 977.907 kb on - strand, within BWI76_RS04685 at 977.907 kb on - strand, within BWI76_RS04685 at 977.907 kb on - strand, within BWI76_RS04685 at 977.907 kb on - strand, within BWI76_RS04685 at 977.907 kb on - strand, within BWI76_RS04685 at 977.950 kb on - strand at 977.950 kb on - strand at 977.970 kb on - strand at 977.970 kb on - strand at 977.970 kb on - strand at 977.970 kb on - strand at 977.977 kb on + strand at 977.977 kb on + strand at 977.977 kb on + strand at 977.985 kb on - strand at 977.990 kb on + strand at 977.991 kb on + strand at 977.997 kb on + strand at 978.004 kb on - strand at 978.005 kb on - strand at 978.047 kb on + strand at 978.055 kb on - strand at 978.057 kb on - strand at 978.155 kb on - strand at 978.167 kb on - strand at 978.169 kb on + strand at 978.169 kb on + strand at 978.177 kb on - strand at 978.177 kb on - strand at 978.178 kb on - strand at 978.221 kb on - strand at 978.271 kb on + strand at 978.271 kb on + strand at 978.271 kb on + strand at 978.271 kb on + strand at 978.279 kb on - strand at 978.377 kb on + strand at 978.377 kb on + strand at 978.377 kb on + strand at 978.431 kb on + strand at 978.431 kb on - strand at 978.548 kb on - strand at 978.550 kb on - strand at 978.585 kb on + strand at 978.596 kb on + strand at 978.600 kb on + strand at 978.601 kb on + strand at 978.601 kb on + strand at 978.601 kb on + strand at 978.601 kb on + strand at 978.601 kb on + strand at 978.601 kb on + strand at 978.606 kb on + strand at 978.606 kb on + strand at 978.608 kb on + strand at 978.608 kb on + strand at 978.608 kb on + strand at 978.608 kb on + strand at 978.608 kb on - strand at 978.608 kb on - strand at 978.609 kb on - strand at 978.609 kb on - strand at 978.616 kb on - strand at 978.616 kb on - strand at 978.627 kb on + strand at 978.627 kb on + strand at 978.627 kb on + strand at 978.627 kb on + strand at 978.627 kb on + strand at 978.635 kb on - strand at 978.693 kb on + strand, within BWI76_RS04690 at 978.700 kb on - strand, within BWI76_RS04690 at 978.700 kb on - strand, within BWI76_RS04690 at 978.701 kb on - strand, within BWI76_RS04690 at 978.703 kb on + strand, within BWI76_RS04690 at 978.713 kb on + strand at 978.713 kb on + strand, within BWI76_RS04690 at 978.713 kb on + strand, within BWI76_RS04690 at 978.713 kb on + strand, within BWI76_RS04690 at 978.721 kb on - strand, within BWI76_RS04690 at 978.728 kb on - strand, within BWI76_RS04690 at 978.728 kb on - strand, within BWI76_RS04690
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 975,438 + BWI76_RS04675 0.32 -3.0 975,473 + BWI76_RS04675 0.35 -3.5 975,788 - BWI76_RS04675 0.64 -1.0 975,833 - BWI76_RS04675 0.68 -1.7 975,841 - BWI76_RS04675 0.69 +0.5 975,899 + BWI76_RS04675 0.74 -1.1 975,900 - BWI76_RS04675 0.74 -3.5 976,189 + -0.7 976,321 - -1.3 976,321 - +0.5 976,368 - BWI76_RS04680 0.12 -1.9 976,375 + BWI76_RS04680 0.12 -5.1 976,383 - BWI76_RS04680 0.13 -0.6 976,388 - BWI76_RS04680 0.13 -0.5 976,423 - BWI76_RS04680 0.16 +1.1 976,517 + BWI76_RS04680 0.22 +1.1 976,628 + BWI76_RS04680 0.29 -0.9 976,696 - BWI76_RS04680 0.33 -1.7 976,775 + BWI76_RS04680 0.38 -2.7 976,807 - BWI76_RS04680 0.40 -4.8 976,858 + BWI76_RS04680 0.43 -1.4 976,895 - BWI76_RS04680 0.46 -2.1 976,918 + BWI76_RS04680 0.47 +1.1 977,079 - BWI76_RS04680 0.57 -0.5 977,084 - BWI76_RS04680 0.58 -0.7 977,091 + BWI76_RS04680 0.58 -3.0 977,104 - BWI76_RS04680 0.59 -5.3 977,135 - BWI76_RS04680 0.61 +0.1 977,172 + BWI76_RS04680 0.63 -2.3 977,222 + BWI76_RS04680 0.66 -3.3 977,222 + BWI76_RS04680 0.66 -1.4 977,234 - BWI76_RS04680 0.67 -2.2 977,361 - BWI76_RS04680 0.75 -0.9 977,361 - BWI76_RS04680 0.75 +1.1 977,450 + BWI76_RS04680 0.81 -1.7 977,550 + BWI76_RS04680 0.87 -3.4 977,550 + BWI76_RS04680 0.87 -1.9 977,571 + BWI76_RS04680 0.89 -0.7 977,572 - BWI76_RS04680 0.89 -0.6 977,579 - BWI76_RS04680 0.89 -1.1 977,627 + +1.7 977,627 + +0.3 977,632 + -0.9 977,686 - -2.2 977,688 + -2.5 977,688 + +0.1 977,702 + -3.1 977,707 - -1.2 977,707 - +1.1 977,710 - -1.6 977,712 + -3.0 977,712 + -4.8 977,720 - -1.1 977,720 - -1.2 977,760 - -2.1 977,760 - -2.5 977,769 + -1.9 977,771 + -1.4 977,777 - +0.4 977,781 - +0.3 977,820 + -0.5 977,844 + -3.9 977,859 + BWI76_RS04685 0.15 -5.3 977,866 + BWI76_RS04685 0.23 -1.7 977,866 + BWI76_RS04685 0.23 -1.7 977,874 - BWI76_RS04685 0.32 -3.5 977,899 + BWI76_RS04685 0.61 -1.6 977,899 + BWI76_RS04685 0.61 -2.5 977,899 + BWI76_RS04685 0.61 -4.1 977,899 + BWI76_RS04685 0.61 -1.5 977,899 - BWI76_RS04685 0.61 -0.3 977,899 - BWI76_RS04685 0.61 -1.9 977,907 - BWI76_RS04685 0.70 +0.1 977,907 - BWI76_RS04685 0.70 -2.1 977,907 - BWI76_RS04685 0.70 -0.2 977,907 - BWI76_RS04685 0.70 -3.2 977,907 - BWI76_RS04685 0.70 +0.5 977,950 - +0.2 977,950 - +0.7 977,970 - +0.4 977,970 - -0.1 977,970 - -0.2 977,970 - -0.2 977,977 + +1.1 977,977 + -0.3 977,977 + -1.2 977,985 - -0.8 977,990 + -1.5 977,991 + -2.5 977,997 + -1.6 978,004 - +1.0 978,005 - +0.1 978,047 + +0.6 978,055 - +0.0 978,057 - -1.9 978,155 - +1.1 978,167 - -0.9 978,169 + -1.3 978,169 + -0.5 978,177 - +0.1 978,177 - -1.2 978,178 - -2.3 978,221 - -1.2 978,271 + +0.9 978,271 + -0.9 978,271 + +1.1 978,271 + +0.1 978,279 - -2.6 978,377 + +0.1 978,377 + -0.8 978,377 + -1.7 978,431 + +0.1 978,431 - -2.1 978,548 - -1.0 978,550 - +1.1 978,585 + +1.1 978,596 + +1.1 978,600 + -1.9 978,601 + +1.1 978,601 + -0.5 978,601 + -0.8 978,601 + +0.2 978,601 + -1.5 978,601 + -1.2 978,606 + -2.2 978,606 + +0.4 978,608 + +0.1 978,608 + -1.5 978,608 + +0.1 978,608 + +1.5 978,608 - +0.2 978,608 - -2.2 978,609 - -1.9 978,609 - -1.2 978,616 - -1.2 978,616 - -0.6 978,627 + -1.9 978,627 + +0.1 978,627 + -0.5 978,627 + -1.5 978,627 + +1.6 978,635 - +0.1 978,693 + BWI76_RS04690 0.15 +0.3 978,700 - BWI76_RS04690 0.15 -0.3 978,700 - BWI76_RS04690 0.15 -1.6 978,701 - BWI76_RS04690 0.15 -0.6 978,703 + BWI76_RS04690 0.16 -2.1 978,713 + -1.2 978,713 + BWI76_RS04690 0.17 +1.4 978,713 + BWI76_RS04690 0.17 +1.1 978,713 + BWI76_RS04690 0.17 +0.1 978,721 - BWI76_RS04690 0.18 -1.7 978,728 - BWI76_RS04690 0.18 +0.3 978,728 - BWI76_RS04690 0.18 +0.7
Or see this region's nucleotide sequence