Experiment: RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04670 and BWI76_RS04675 are separated by 2 nucleotides BWI76_RS04675 and BWI76_RS04680 overlap by 1 nucleotides
BWI76_RS04670: BWI76_RS04670 - 3-isopropylmalate dehydratase large subunit, at 973,685 to 975,085
_RS04670
BWI76_RS04675: BWI76_RS04675 - 3-isopropylmalate dehydrogenase, at 975,088 to 976,179
_RS04675
BWI76_RS04680: BWI76_RS04680 - 2-isopropylmalate synthase, at 976,179 to 977,750
_RS04680
Position (kb) 975
976
977 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 974.120 kb on + strand, within BWI76_RS04670 at 974.272 kb on - strand, within BWI76_RS04670 at 974.372 kb on + strand, within BWI76_RS04670 at 974.395 kb on - strand, within BWI76_RS04670 at 974.433 kb on + strand, within BWI76_RS04670 at 974.439 kb on - strand, within BWI76_RS04670 at 974.477 kb on + strand, within BWI76_RS04670 at 974.480 kb on - strand, within BWI76_RS04670 at 974.741 kb on - strand at 974.797 kb on + strand, within BWI76_RS04670 at 974.894 kb on + strand, within BWI76_RS04670 at 974.924 kb on - strand, within BWI76_RS04670 at 974.924 kb on - strand, within BWI76_RS04670 at 975.006 kb on + strand at 975.145 kb on - strand at 975.438 kb on + strand, within BWI76_RS04675 at 975.473 kb on + strand, within BWI76_RS04675 at 975.788 kb on - strand, within BWI76_RS04675 at 975.833 kb on - strand, within BWI76_RS04675 at 975.841 kb on - strand, within BWI76_RS04675 at 975.899 kb on + strand, within BWI76_RS04675 at 975.900 kb on - strand, within BWI76_RS04675 at 976.189 kb on + strand at 976.321 kb on - strand at 976.321 kb on - strand at 976.368 kb on - strand, within BWI76_RS04680 at 976.375 kb on + strand, within BWI76_RS04680 at 976.383 kb on - strand, within BWI76_RS04680 at 976.388 kb on - strand, within BWI76_RS04680 at 976.423 kb on - strand, within BWI76_RS04680 at 976.517 kb on + strand, within BWI76_RS04680 at 976.628 kb on + strand, within BWI76_RS04680 at 976.696 kb on - strand, within BWI76_RS04680 at 976.775 kb on + strand, within BWI76_RS04680 at 976.807 kb on - strand, within BWI76_RS04680 at 976.858 kb on + strand, within BWI76_RS04680 at 976.895 kb on - strand, within BWI76_RS04680 at 976.918 kb on + strand, within BWI76_RS04680 at 977.079 kb on - strand, within BWI76_RS04680 at 977.084 kb on - strand, within BWI76_RS04680 at 977.091 kb on + strand, within BWI76_RS04680 at 977.104 kb on - strand, within BWI76_RS04680 at 977.135 kb on - strand, within BWI76_RS04680 at 977.172 kb on + strand, within BWI76_RS04680
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 974,120 + BWI76_RS04670 0.31 -1.0 974,272 - BWI76_RS04670 0.42 -1.2 974,372 + BWI76_RS04670 0.49 -2.2 974,395 - BWI76_RS04670 0.51 -2.2 974,433 + BWI76_RS04670 0.53 -1.1 974,439 - BWI76_RS04670 0.54 -2.2 974,477 + BWI76_RS04670 0.57 -1.0 974,480 - BWI76_RS04670 0.57 -1.6 974,741 - -2.0 974,797 + BWI76_RS04670 0.79 -3.8 974,894 + BWI76_RS04670 0.86 -1.4 974,924 - BWI76_RS04670 0.88 -2.9 974,924 - BWI76_RS04670 0.88 -1.7 975,006 + -1.2 975,145 - +0.1 975,438 + BWI76_RS04675 0.32 -3.0 975,473 + BWI76_RS04675 0.35 -3.5 975,788 - BWI76_RS04675 0.64 -1.0 975,833 - BWI76_RS04675 0.68 -1.7 975,841 - BWI76_RS04675 0.69 +0.5 975,899 + BWI76_RS04675 0.74 -1.1 975,900 - BWI76_RS04675 0.74 -3.5 976,189 + -0.7 976,321 - -1.3 976,321 - +0.5 976,368 - BWI76_RS04680 0.12 -1.9 976,375 + BWI76_RS04680 0.12 -5.1 976,383 - BWI76_RS04680 0.13 -0.6 976,388 - BWI76_RS04680 0.13 -0.5 976,423 - BWI76_RS04680 0.16 +1.1 976,517 + BWI76_RS04680 0.22 +1.1 976,628 + BWI76_RS04680 0.29 -0.9 976,696 - BWI76_RS04680 0.33 -1.7 976,775 + BWI76_RS04680 0.38 -2.7 976,807 - BWI76_RS04680 0.40 -4.8 976,858 + BWI76_RS04680 0.43 -1.4 976,895 - BWI76_RS04680 0.46 -2.1 976,918 + BWI76_RS04680 0.47 +1.1 977,079 - BWI76_RS04680 0.57 -0.5 977,084 - BWI76_RS04680 0.58 -0.7 977,091 + BWI76_RS04680 0.58 -3.0 977,104 - BWI76_RS04680 0.59 -5.3 977,135 - BWI76_RS04680 0.61 +0.1 977,172 + BWI76_RS04680 0.63 -2.3
Or see this region's nucleotide sequence