Experiment: RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04665 and BWI76_RS04670 are separated by 10 nucleotides BWI76_RS04670 and BWI76_RS04675 are separated by 2 nucleotides
BWI76_RS04665: BWI76_RS04665 - 3-isopropylmalate dehydratase small subunit, at 973,069 to 973,674
_RS04665
BWI76_RS04670: BWI76_RS04670 - 3-isopropylmalate dehydratase large subunit, at 973,685 to 975,085
_RS04670
BWI76_RS04675: BWI76_RS04675 - 3-isopropylmalate dehydrogenase, at 975,088 to 976,179
_RS04675
Position (kb) 973
974
975
976 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 972.692 kb on - strand at 972.704 kb on + strand at 972.704 kb on + strand at 972.704 kb on + strand at 972.704 kb on + strand at 972.704 kb on + strand at 972.704 kb on + strand at 972.704 kb on + strand at 972.704 kb on + strand at 972.704 kb on + strand at 972.704 kb on + strand at 972.704 kb on + strand at 972.704 kb on + strand at 972.704 kb on + strand at 972.704 kb on + strand at 972.704 kb on + strand at 972.704 kb on + strand at 972.704 kb on + strand at 972.704 kb on + strand at 972.712 kb on - strand at 972.712 kb on - strand at 972.712 kb on - strand at 972.712 kb on - strand at 972.712 kb on - strand at 972.712 kb on - strand at 972.738 kb on + strand at 972.788 kb on + strand at 972.895 kb on + strand at 973.046 kb on - strand at 973.046 kb on - strand at 973.049 kb on + strand at 973.049 kb on + strand at 973.049 kb on + strand at 973.049 kb on + strand at 973.049 kb on + strand at 973.049 kb on + strand at 973.049 kb on + strand at 973.049 kb on + strand at 973.049 kb on + strand at 973.049 kb on + strand at 973.049 kb on + strand at 973.049 kb on + strand at 973.049 kb on + strand at 973.049 kb on + strand at 973.049 kb on + strand at 973.049 kb on + strand at 973.049 kb on + strand at 973.050 kb on - strand at 973.050 kb on - strand at 973.055 kb on - strand at 973.057 kb on - strand at 973.057 kb on - strand at 973.057 kb on - strand at 973.057 kb on - strand at 973.057 kb on - strand at 973.057 kb on - strand at 973.057 kb on - strand at 973.057 kb on - strand at 973.057 kb on - strand at 973.057 kb on - strand at 973.075 kb on + strand at 973.083 kb on - strand at 973.083 kb on - strand at 973.089 kb on + strand at 973.116 kb on - strand at 973.128 kb on + strand at 973.128 kb on + strand at 973.128 kb on + strand at 973.128 kb on + strand at 973.128 kb on + strand at 973.128 kb on + strand at 973.128 kb on - strand at 973.129 kb on + strand at 973.129 kb on + strand at 973.134 kb on + strand, within BWI76_RS04665 at 973.134 kb on + strand, within BWI76_RS04665 at 973.134 kb on + strand, within BWI76_RS04665 at 973.134 kb on + strand, within BWI76_RS04665 at 973.134 kb on + strand, within BWI76_RS04665 at 973.134 kb on + strand, within BWI76_RS04665 at 973.134 kb on + strand, within BWI76_RS04665 at 973.134 kb on + strand, within BWI76_RS04665 at 973.134 kb on + strand, within BWI76_RS04665 at 973.134 kb on + strand, within BWI76_RS04665 at 973.134 kb on + strand, within BWI76_RS04665 at 973.136 kb on - strand, within BWI76_RS04665 at 973.136 kb on - strand, within BWI76_RS04665 at 973.136 kb on - strand, within BWI76_RS04665 at 973.136 kb on - strand, within BWI76_RS04665 at 973.136 kb on - strand, within BWI76_RS04665 at 973.139 kb on + strand, within BWI76_RS04665 at 973.139 kb on + strand, within BWI76_RS04665 at 973.142 kb on - strand, within BWI76_RS04665 at 973.142 kb on - strand, within BWI76_RS04665 at 973.142 kb on - strand, within BWI76_RS04665 at 973.142 kb on - strand, within BWI76_RS04665 at 973.142 kb on - strand, within BWI76_RS04665 at 973.142 kb on - strand, within BWI76_RS04665 at 973.142 kb on - strand, within BWI76_RS04665 at 973.142 kb on - strand, within BWI76_RS04665 at 973.142 kb on - strand, within BWI76_RS04665 at 973.142 kb on - strand, within BWI76_RS04665 at 973.143 kb on + strand, within BWI76_RS04665 at 973.143 kb on + strand, within BWI76_RS04665 at 973.144 kb on + strand, within BWI76_RS04665 at 973.144 kb on + strand, within BWI76_RS04665 at 973.144 kb on + strand, within BWI76_RS04665 at 973.144 kb on + strand, within BWI76_RS04665 at 973.144 kb on + strand at 973.147 kb on - strand, within BWI76_RS04665 at 973.147 kb on - strand, within BWI76_RS04665 at 973.151 kb on + strand, within BWI76_RS04665 at 973.151 kb on + strand, within BWI76_RS04665 at 973.152 kb on - strand, within BWI76_RS04665 at 973.152 kb on - strand, within BWI76_RS04665 at 973.152 kb on - strand, within BWI76_RS04665 at 973.152 kb on - strand, within BWI76_RS04665 at 973.152 kb on - strand, within BWI76_RS04665 at 973.152 kb on - strand, within BWI76_RS04665 at 973.152 kb on - strand, within BWI76_RS04665 at 973.152 kb on - strand, within BWI76_RS04665 at 973.192 kb on + strand, within BWI76_RS04665 at 973.211 kb on + strand, within BWI76_RS04665 at 973.211 kb on + strand, within BWI76_RS04665 at 973.219 kb on - strand, within BWI76_RS04665 at 973.219 kb on - strand, within BWI76_RS04665 at 973.256 kb on + strand, within BWI76_RS04665 at 973.279 kb on - strand, within BWI76_RS04665 at 973.303 kb on - strand, within BWI76_RS04665 at 973.361 kb on - strand, within BWI76_RS04665 at 973.407 kb on + strand, within BWI76_RS04665 at 973.463 kb on - strand, within BWI76_RS04665 at 973.466 kb on + strand, within BWI76_RS04665 at 973.466 kb on + strand, within BWI76_RS04665 at 973.499 kb on + strand, within BWI76_RS04665 at 973.554 kb on + strand, within BWI76_RS04665 at 973.670 kb on + strand at 973.751 kb on + strand at 973.759 kb on - strand at 973.852 kb on - strand, within BWI76_RS04670 at 973.891 kb on - strand, within BWI76_RS04670 at 973.945 kb on + strand, within BWI76_RS04670 at 974.038 kb on + strand, within BWI76_RS04670 at 974.120 kb on + strand, within BWI76_RS04670 at 974.272 kb on - strand, within BWI76_RS04670 at 974.372 kb on + strand, within BWI76_RS04670 at 974.395 kb on - strand, within BWI76_RS04670 at 974.433 kb on + strand, within BWI76_RS04670 at 974.439 kb on - strand, within BWI76_RS04670 at 974.477 kb on + strand, within BWI76_RS04670 at 974.480 kb on - strand, within BWI76_RS04670 at 974.741 kb on - strand at 974.797 kb on + strand, within BWI76_RS04670 at 974.894 kb on + strand, within BWI76_RS04670 at 974.924 kb on - strand, within BWI76_RS04670 at 974.924 kb on - strand, within BWI76_RS04670 at 975.006 kb on + strand at 975.145 kb on - strand at 975.438 kb on + strand, within BWI76_RS04675 at 975.473 kb on + strand, within BWI76_RS04675 at 975.788 kb on - strand, within BWI76_RS04675 at 975.833 kb on - strand, within BWI76_RS04675 at 975.841 kb on - strand, within BWI76_RS04675 at 975.899 kb on + strand, within BWI76_RS04675 at 975.900 kb on - strand, within BWI76_RS04675
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 972,692 - -1.4 972,704 + -0.0 972,704 + +2.1 972,704 + -0.7 972,704 + -1.2 972,704 + -1.2 972,704 + -1.2 972,704 + -0.5 972,704 + -0.5 972,704 + -2.0 972,704 + +0.0 972,704 + -0.5 972,704 + -1.2 972,704 + +0.1 972,704 + -1.7 972,704 + -1.2 972,704 + +0.5 972,704 + +0.9 972,704 + +0.0 972,712 - -1.9 972,712 - -0.5 972,712 - -0.5 972,712 - -1.5 972,712 - +1.1 972,712 - -0.5 972,738 + -2.0 972,788 + +0.9 972,895 + -2.1 973,046 - -2.4 973,046 - -0.0 973,049 + +0.1 973,049 + -0.1 973,049 + +0.2 973,049 + -1.2 973,049 + -1.2 973,049 + +0.7 973,049 + +0.1 973,049 + +0.7 973,049 + -3.9 973,049 + -1.2 973,049 + -0.3 973,049 + +0.7 973,049 + -0.8 973,049 + -0.1 973,049 + -0.5 973,049 + -1.7 973,049 + -0.1 973,050 - -0.5 973,050 - +0.1 973,055 - -0.3 973,057 - +0.4 973,057 - -1.2 973,057 - +1.3 973,057 - +0.7 973,057 - -1.3 973,057 - -0.7 973,057 - -0.9 973,057 - -0.1 973,057 - -0.2 973,057 - -3.5 973,075 + +1.9 973,083 - -2.9 973,083 - +0.1 973,089 + +0.7 973,116 - -1.7 973,128 + -0.9 973,128 + -3.6 973,128 + -1.3 973,128 + -0.3 973,128 + -2.1 973,128 + -0.3 973,128 - -0.6 973,129 + -0.8 973,129 + -1.5 973,134 + BWI76_RS04665 0.11 -2.3 973,134 + BWI76_RS04665 0.11 -0.8 973,134 + BWI76_RS04665 0.11 +1.1 973,134 + BWI76_RS04665 0.11 -2.3 973,134 + BWI76_RS04665 0.11 -1.7 973,134 + BWI76_RS04665 0.11 -3.2 973,134 + BWI76_RS04665 0.11 -3.4 973,134 + BWI76_RS04665 0.11 -0.5 973,134 + BWI76_RS04665 0.11 -2.8 973,134 + BWI76_RS04665 0.11 -1.9 973,134 + BWI76_RS04665 0.11 -3.8 973,136 - BWI76_RS04665 0.11 -4.5 973,136 - BWI76_RS04665 0.11 -5.7 973,136 - BWI76_RS04665 0.11 +1.1 973,136 - BWI76_RS04665 0.11 -1.9 973,136 - BWI76_RS04665 0.11 -1.0 973,139 + BWI76_RS04665 0.12 -0.6 973,139 + BWI76_RS04665 0.12 +1.1 973,142 - BWI76_RS04665 0.12 -2.5 973,142 - BWI76_RS04665 0.12 -1.8 973,142 - BWI76_RS04665 0.12 -0.5 973,142 - BWI76_RS04665 0.12 -2.2 973,142 - BWI76_RS04665 0.12 -2.1 973,142 - BWI76_RS04665 0.12 -1.9 973,142 - BWI76_RS04665 0.12 -3.0 973,142 - BWI76_RS04665 0.12 -1.1 973,142 - BWI76_RS04665 0.12 -2.3 973,142 - BWI76_RS04665 0.12 -2.0 973,143 + BWI76_RS04665 0.12 -3.7 973,143 + BWI76_RS04665 0.12 -2.5 973,144 + BWI76_RS04665 0.12 +2.1 973,144 + BWI76_RS04665 0.12 -1.4 973,144 + BWI76_RS04665 0.12 -2.2 973,144 + BWI76_RS04665 0.12 -0.9 973,144 + -0.5 973,147 - BWI76_RS04665 0.13 -4.0 973,147 - BWI76_RS04665 0.13 -1.4 973,151 + BWI76_RS04665 0.14 -2.8 973,151 + BWI76_RS04665 0.14 -2.0 973,152 - BWI76_RS04665 0.14 -2.8 973,152 - BWI76_RS04665 0.14 -2.6 973,152 - BWI76_RS04665 0.14 +0.5 973,152 - BWI76_RS04665 0.14 -4.7 973,152 - BWI76_RS04665 0.14 -0.5 973,152 - BWI76_RS04665 0.14 -0.9 973,152 - BWI76_RS04665 0.14 -2.7 973,152 - BWI76_RS04665 0.14 -2.9 973,192 + BWI76_RS04665 0.20 +1.1 973,211 + BWI76_RS04665 0.23 -1.5 973,211 + BWI76_RS04665 0.23 -1.8 973,219 - BWI76_RS04665 0.25 -3.3 973,219 - BWI76_RS04665 0.25 -5.7 973,256 + BWI76_RS04665 0.31 -1.5 973,279 - BWI76_RS04665 0.35 -2.2 973,303 - BWI76_RS04665 0.39 -2.5 973,361 - BWI76_RS04665 0.48 -1.4 973,407 + BWI76_RS04665 0.56 -1.6 973,463 - BWI76_RS04665 0.65 -1.5 973,466 + BWI76_RS04665 0.66 -1.9 973,466 + BWI76_RS04665 0.66 -1.2 973,499 + BWI76_RS04665 0.71 -1.6 973,554 + BWI76_RS04665 0.80 -0.6 973,670 + -2.6 973,751 + -3.1 973,759 - -4.4 973,852 - BWI76_RS04670 0.12 -2.4 973,891 - BWI76_RS04670 0.15 -4.3 973,945 + BWI76_RS04670 0.19 -1.7 974,038 + BWI76_RS04670 0.25 +0.1 974,120 + BWI76_RS04670 0.31 -1.0 974,272 - BWI76_RS04670 0.42 -1.2 974,372 + BWI76_RS04670 0.49 -2.2 974,395 - BWI76_RS04670 0.51 -2.2 974,433 + BWI76_RS04670 0.53 -1.1 974,439 - BWI76_RS04670 0.54 -2.2 974,477 + BWI76_RS04670 0.57 -1.0 974,480 - BWI76_RS04670 0.57 -1.6 974,741 - -2.0 974,797 + BWI76_RS04670 0.79 -3.8 974,894 + BWI76_RS04670 0.86 -1.4 974,924 - BWI76_RS04670 0.88 -2.9 974,924 - BWI76_RS04670 0.88 -1.7 975,006 + -1.2 975,145 - +0.1 975,438 + BWI76_RS04675 0.32 -3.0 975,473 + BWI76_RS04675 0.35 -3.5 975,788 - BWI76_RS04675 0.64 -1.0 975,833 - BWI76_RS04675 0.68 -1.7 975,841 - BWI76_RS04675 0.69 +0.5 975,899 + BWI76_RS04675 0.74 -1.1 975,900 - BWI76_RS04675 0.74 -3.5
Or see this region's nucleotide sequence