Experiment: RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04465 and BWI76_RS04470 are separated by 18 nucleotides BWI76_RS04470 and BWI76_RS04475 are separated by 138 nucleotides
BWI76_RS04465: BWI76_RS04465 - carbamoyl-phosphate synthase small subunit, at 922,546 to 923,694
_RS04465
BWI76_RS04470: BWI76_RS04470 - carbamoyl phosphate synthase large subunit, at 923,713 to 926,937
_RS04470
BWI76_RS04475: BWI76_RS04475 - ROK family protein, at 927,076 to 927,960
_RS04475
Position (kb) 923
924
925
926
927 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 922.828 kb on + strand, within BWI76_RS04465 at 922.836 kb on + strand, within BWI76_RS04465 at 922.836 kb on - strand, within BWI76_RS04465 at 922.897 kb on - strand, within BWI76_RS04465 at 923.052 kb on - strand, within BWI76_RS04465 at 923.206 kb on + strand, within BWI76_RS04465 at 923.623 kb on - strand at 923.628 kb on - strand at 923.794 kb on - strand at 923.808 kb on + strand at 923.816 kb on - strand at 923.855 kb on + strand at 923.862 kb on + strand at 923.879 kb on - strand at 923.995 kb on + strand at 923.995 kb on + strand at 924.003 kb on - strand at 924.256 kb on + strand, within BWI76_RS04470 at 924.264 kb on - strand at 924.575 kb on - strand, within BWI76_RS04470 at 924.717 kb on + strand, within BWI76_RS04470 at 924.748 kb on - strand, within BWI76_RS04470 at 924.751 kb on - strand, within BWI76_RS04470 at 924.760 kb on - strand, within BWI76_RS04470 at 924.914 kb on + strand, within BWI76_RS04470 at 925.097 kb on + strand, within BWI76_RS04470 at 925.338 kb on + strand, within BWI76_RS04470 at 925.346 kb on - strand, within BWI76_RS04470 at 925.547 kb on + strand, within BWI76_RS04470 at 925.706 kb on + strand, within BWI76_RS04470 at 925.931 kb on - strand, within BWI76_RS04470 at 926.164 kb on - strand, within BWI76_RS04470 at 926.332 kb on - strand, within BWI76_RS04470 at 926.473 kb on + strand, within BWI76_RS04470 at 926.481 kb on - strand, within BWI76_RS04470 at 926.610 kb on + strand, within BWI76_RS04470 at 926.817 kb on + strand at 926.817 kb on + strand at 926.903 kb on - strand at 927.074 kb on - strand at 927.433 kb on + strand, within BWI76_RS04475 at 927.433 kb on + strand, within BWI76_RS04475 at 927.441 kb on - strand, within BWI76_RS04475 at 927.613 kb on - strand, within BWI76_RS04475 at 927.747 kb on + strand, within BWI76_RS04475 at 927.752 kb on + strand, within BWI76_RS04475 at 927.815 kb on + strand, within BWI76_RS04475 at 927.823 kb on - strand, within BWI76_RS04475 at 927.907 kb on + strand at 927.910 kb on - strand at 927.915 kb on - strand at 927.922 kb on + strand at 927.930 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 922,828 + BWI76_RS04465 0.25 -3.8 922,836 + BWI76_RS04465 0.25 -5.3 922,836 - BWI76_RS04465 0.25 -2.4 922,897 - BWI76_RS04465 0.31 -1.7 923,052 - BWI76_RS04465 0.44 -3.0 923,206 + BWI76_RS04465 0.57 -1.2 923,623 - -2.7 923,628 - -3.2 923,794 - -2.9 923,808 + -2.2 923,816 - +1.1 923,855 + -3.7 923,862 + -2.0 923,879 - -2.7 923,995 + +0.1 923,995 + -1.4 924,003 - -3.4 924,256 + BWI76_RS04470 0.17 -2.4 924,264 - -1.2 924,575 - BWI76_RS04470 0.27 -4.0 924,717 + BWI76_RS04470 0.31 +0.1 924,748 - BWI76_RS04470 0.32 +0.1 924,751 - BWI76_RS04470 0.32 -2.4 924,760 - BWI76_RS04470 0.32 -2.6 924,914 + BWI76_RS04470 0.37 -2.6 925,097 + BWI76_RS04470 0.43 -3.0 925,338 + BWI76_RS04470 0.50 +1.1 925,346 - BWI76_RS04470 0.51 -0.9 925,547 + BWI76_RS04470 0.57 +0.1 925,706 + BWI76_RS04470 0.62 -4.4 925,931 - BWI76_RS04470 0.69 -1.9 926,164 - BWI76_RS04470 0.76 -4.7 926,332 - BWI76_RS04470 0.81 -5.3 926,473 + BWI76_RS04470 0.86 +0.1 926,481 - BWI76_RS04470 0.86 -3.9 926,610 + BWI76_RS04470 0.90 -3.5 926,817 + -2.7 926,817 + -3.7 926,903 - -2.6 927,074 - +0.3 927,433 + BWI76_RS04475 0.40 +1.1 927,433 + BWI76_RS04475 0.40 +1.1 927,441 - BWI76_RS04475 0.41 -0.1 927,613 - BWI76_RS04475 0.61 -0.3 927,747 + BWI76_RS04475 0.76 -0.4 927,752 + BWI76_RS04475 0.76 -0.6 927,815 + BWI76_RS04475 0.84 +1.0 927,823 - BWI76_RS04475 0.84 +0.6 927,907 + +0.4 927,910 - +0.5 927,915 - -0.2 927,922 + +0.6 927,930 - +1.1
Or see this region's nucleotide sequence