Experiment: RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04235 and BWI76_RS04240 are separated by 212 nucleotides BWI76_RS04240 and BWI76_RS04245 are separated by 81 nucleotides BWI76_RS04245 and BWI76_RS04250 are separated by 1 nucleotides BWI76_RS04250 and BWI76_RS04255 are separated by 3 nucleotides
BWI76_RS04235: BWI76_RS04235 - tRNA/rRNA methyltransferase, at 871,476 to 872,162
_RS04235
BWI76_RS04240: BWI76_RS04240 - thr operon leader peptide, at 872,375 to 872,443
_RS04240
BWI76_RS04245: BWI76_RS04245 - bifunctional aspartate kinase/homoserine dehydrogenase I, at 872,525 to 874,987
_RS04245
BWI76_RS04250: BWI76_RS04250 - homoserine kinase, at 874,989 to 875,918
_RS04250
BWI76_RS04255: BWI76_RS04255 - threonine synthase, at 875,922 to 877,202
_RS04255
Position (kb) 872
873
874
875 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 871.620 kb on - strand, within BWI76_RS04235 at 871.624 kb on + strand, within BWI76_RS04235 at 871.632 kb on - strand, within BWI76_RS04235 at 871.784 kb on + strand, within BWI76_RS04235 at 871.868 kb on - strand, within BWI76_RS04235 at 871.870 kb on - strand, within BWI76_RS04235 at 871.886 kb on + strand, within BWI76_RS04235 at 871.903 kb on + strand, within BWI76_RS04235 at 871.903 kb on + strand, within BWI76_RS04235 at 871.911 kb on - strand, within BWI76_RS04235 at 871.930 kb on - strand, within BWI76_RS04235 at 872.053 kb on + strand, within BWI76_RS04235 at 872.061 kb on - strand, within BWI76_RS04235 at 872.061 kb on - strand, within BWI76_RS04235 at 872.071 kb on + strand, within BWI76_RS04235 at 872.079 kb on - strand, within BWI76_RS04235 at 872.110 kb on + strand at 872.110 kb on + strand at 872.118 kb on + strand at 872.123 kb on - strand at 872.337 kb on + strand at 872.362 kb on + strand at 872.368 kb on - strand at 872.433 kb on - strand, within BWI76_RS04240 at 872.433 kb on - strand, within BWI76_RS04240 at 872.473 kb on + strand at 872.473 kb on + strand at 872.473 kb on + strand at 872.475 kb on + strand at 872.475 kb on + strand at 872.481 kb on - strand at 872.515 kb on + strand at 872.612 kb on - strand at 872.625 kb on + strand at 872.754 kb on - strand at 872.844 kb on + strand, within BWI76_RS04245 at 872.844 kb on + strand, within BWI76_RS04245 at 872.844 kb on + strand, within BWI76_RS04245 at 872.844 kb on + strand, within BWI76_RS04245 at 872.852 kb on - strand, within BWI76_RS04245 at 872.852 kb on - strand, within BWI76_RS04245 at 872.852 kb on - strand, within BWI76_RS04245 at 872.852 kb on - strand, within BWI76_RS04245 at 872.852 kb on - strand, within BWI76_RS04245 at 872.882 kb on - strand, within BWI76_RS04245 at 872.935 kb on + strand, within BWI76_RS04245 at 873.226 kb on - strand, within BWI76_RS04245 at 873.292 kb on - strand, within BWI76_RS04245 at 873.295 kb on + strand, within BWI76_RS04245 at 873.592 kb on - strand, within BWI76_RS04245 at 873.696 kb on + strand, within BWI76_RS04245 at 873.740 kb on - strand, within BWI76_RS04245 at 873.779 kb on + strand, within BWI76_RS04245 at 873.779 kb on + strand, within BWI76_RS04245 at 873.787 kb on - strand, within BWI76_RS04245 at 873.815 kb on + strand, within BWI76_RS04245 at 873.909 kb on - strand, within BWI76_RS04245 at 873.916 kb on + strand, within BWI76_RS04245 at 874.039 kb on + strand, within BWI76_RS04245 at 874.039 kb on + strand, within BWI76_RS04245 at 874.082 kb on - strand, within BWI76_RS04245 at 874.282 kb on + strand, within BWI76_RS04245 at 874.290 kb on + strand, within BWI76_RS04245 at 874.435 kb on - strand, within BWI76_RS04245 at 874.480 kb on - strand, within BWI76_RS04245 at 874.490 kb on - strand, within BWI76_RS04245 at 874.654 kb on - strand, within BWI76_RS04245 at 874.675 kb on - strand, within BWI76_RS04245 at 874.905 kb on + strand at 874.949 kb on + strand at 875.101 kb on - strand, within BWI76_RS04250 at 875.266 kb on + strand, within BWI76_RS04250 at 875.274 kb on - strand, within BWI76_RS04250 at 875.422 kb on - strand, within BWI76_RS04250 at 875.516 kb on - strand, within BWI76_RS04250 at 875.539 kb on - strand at 875.587 kb on + strand, within BWI76_RS04250 at 875.595 kb on - strand, within BWI76_RS04250 at 875.599 kb on + strand, within BWI76_RS04250 at 875.615 kb on - strand, within BWI76_RS04250 at 875.846 kb on - strand at 875.962 kb on + strand at 875.965 kb on - strand at 875.970 kb on - strand at 875.987 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 871,620 - BWI76_RS04235 0.21 +0.2 871,624 + BWI76_RS04235 0.22 +0.6 871,632 - BWI76_RS04235 0.23 +1.2 871,784 + BWI76_RS04235 0.45 -0.3 871,868 - BWI76_RS04235 0.57 -0.3 871,870 - BWI76_RS04235 0.57 +0.3 871,886 + BWI76_RS04235 0.60 -0.3 871,903 + BWI76_RS04235 0.62 -1.5 871,903 + BWI76_RS04235 0.62 +0.3 871,911 - BWI76_RS04235 0.63 -1.6 871,930 - BWI76_RS04235 0.66 -1.2 872,053 + BWI76_RS04235 0.84 +1.0 872,061 - BWI76_RS04235 0.85 +0.6 872,061 - BWI76_RS04235 0.85 +0.8 872,071 + BWI76_RS04235 0.87 -0.1 872,079 - BWI76_RS04235 0.88 +1.7 872,110 + +1.4 872,110 + -0.1 872,118 + +3.7 872,123 - +0.5 872,337 + -0.6 872,362 + -0.4 872,368 - +0.2 872,433 - BWI76_RS04240 0.84 -4.2 872,433 - BWI76_RS04240 0.84 +1.2 872,473 + -2.0 872,473 + -0.2 872,473 + +0.3 872,475 + -0.4 872,475 + -1.2 872,481 - -0.4 872,515 + -2.3 872,612 - -3.2 872,625 + -2.5 872,754 - -1.5 872,844 + BWI76_RS04245 0.13 -2.7 872,844 + BWI76_RS04245 0.13 -0.4 872,844 + BWI76_RS04245 0.13 -3.2 872,844 + BWI76_RS04245 0.13 -3.4 872,852 - BWI76_RS04245 0.13 -2.8 872,852 - BWI76_RS04245 0.13 -2.9 872,852 - BWI76_RS04245 0.13 -0.8 872,852 - BWI76_RS04245 0.13 -0.8 872,852 - BWI76_RS04245 0.13 -4.3 872,882 - BWI76_RS04245 0.14 -2.8 872,935 + BWI76_RS04245 0.17 -4.7 873,226 - BWI76_RS04245 0.28 +0.2 873,292 - BWI76_RS04245 0.31 -5.5 873,295 + BWI76_RS04245 0.31 -0.7 873,592 - BWI76_RS04245 0.43 -3.5 873,696 + BWI76_RS04245 0.48 -1.2 873,740 - BWI76_RS04245 0.49 -3.5 873,779 + BWI76_RS04245 0.51 -1.8 873,779 + BWI76_RS04245 0.51 -4.2 873,787 - BWI76_RS04245 0.51 -1.8 873,815 + BWI76_RS04245 0.52 -0.8 873,909 - BWI76_RS04245 0.56 +0.2 873,916 + BWI76_RS04245 0.56 -0.9 874,039 + BWI76_RS04245 0.61 -4.2 874,039 + BWI76_RS04245 0.61 -1.4 874,082 - BWI76_RS04245 0.63 -2.3 874,282 + BWI76_RS04245 0.71 -4.8 874,290 + BWI76_RS04245 0.72 -0.9 874,435 - BWI76_RS04245 0.78 -3.2 874,480 - BWI76_RS04245 0.79 -4.7 874,490 - BWI76_RS04245 0.80 -2.9 874,654 - BWI76_RS04245 0.86 -2.8 874,675 - BWI76_RS04245 0.87 -1.3 874,905 + -3.4 874,949 + -2.7 875,101 - BWI76_RS04250 0.12 -3.1 875,266 + BWI76_RS04250 0.30 -3.4 875,274 - BWI76_RS04250 0.31 -5.3 875,422 - BWI76_RS04250 0.47 -5.6 875,516 - BWI76_RS04250 0.57 -4.7 875,539 - +1.2 875,587 + BWI76_RS04250 0.64 -1.4 875,595 - BWI76_RS04250 0.65 -2.8 875,599 + BWI76_RS04250 0.66 -5.4 875,615 - BWI76_RS04250 0.67 -1.2 875,846 - -3.3 875,962 + -4.3 875,965 - +0.2 875,970 - -3.4 875,987 - -3.4
Or see this region's nucleotide sequence