Experiment: RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04150 and BWI76_RS04155 are separated by 10 nucleotides BWI76_RS04155 and BWI76_RS04160 are separated by 104 nucleotides BWI76_RS04160 and BWI76_RS04165 are separated by 9 nucleotides
BWI76_RS04150: BWI76_RS04150 - lipoate--protein ligase A, at 853,899 to 854,915
_RS04150
BWI76_RS04155: BWI76_RS04155 - membrane protein, at 854,926 to 855,567
_RS04155
BWI76_RS04160: BWI76_RS04160 - phosphoserine phosphatase, at 855,672 to 856,643
_RS04160
BWI76_RS04165: BWI76_RS04165 - DNA repair protein RadA, at 856,653 to 858,035
_RS04165
Position (kb) 855
856
857 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 854.972 kb on - strand at 855.070 kb on + strand, within BWI76_RS04155 at 855.251 kb on - strand, within BWI76_RS04155 at 855.251 kb on - strand, within BWI76_RS04155 at 855.486 kb on + strand, within BWI76_RS04155 at 855.504 kb on - strand at 855.512 kb on - strand at 855.522 kb on - strand at 855.691 kb on + strand at 855.726 kb on + strand at 855.797 kb on + strand, within BWI76_RS04160 at 855.866 kb on - strand, within BWI76_RS04160 at 855.919 kb on + strand, within BWI76_RS04160 at 855.927 kb on - strand, within BWI76_RS04160 at 856.200 kb on - strand, within BWI76_RS04160 at 856.310 kb on + strand, within BWI76_RS04160 at 856.331 kb on - strand, within BWI76_RS04160 at 856.571 kb on + strand at 856.593 kb on + strand at 856.598 kb on + strand at 856.603 kb on - strand at 856.606 kb on - strand at 856.617 kb on + strand at 856.695 kb on + strand at 857.087 kb on - strand, within BWI76_RS04165 at 857.376 kb on - strand, within BWI76_RS04165 at 857.473 kb on + strand, within BWI76_RS04165
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 854,972 - +0.2 855,070 + BWI76_RS04155 0.22 +0.5 855,251 - BWI76_RS04155 0.51 +0.0 855,251 - BWI76_RS04155 0.51 +0.9 855,486 + BWI76_RS04155 0.87 -0.9 855,504 - +0.2 855,512 - +0.8 855,522 - +0.0 855,691 + +0.6 855,726 + -2.1 855,797 + BWI76_RS04160 0.13 -0.4 855,866 - BWI76_RS04160 0.20 -1.4 855,919 + BWI76_RS04160 0.25 -2.6 855,927 - BWI76_RS04160 0.26 -2.7 856,200 - BWI76_RS04160 0.54 +0.9 856,310 + BWI76_RS04160 0.66 -2.7 856,331 - BWI76_RS04160 0.68 +1.2 856,571 + -2.1 856,593 + -1.1 856,598 + +1.2 856,603 - -0.8 856,606 - -0.4 856,617 + -3.3 856,695 + -0.3 857,087 - BWI76_RS04165 0.31 +0.6 857,376 - BWI76_RS04165 0.52 -0.2 857,473 + BWI76_RS04165 0.59 +1.1
Or see this region's nucleotide sequence