Experiment: RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03645 and BWI76_RS03650 are separated by 451 nucleotides BWI76_RS03650 and BWI76_RS03655 overlap by 2 nucleotides BWI76_RS03655 and BWI76_RS03660 overlap by 29 nucleotides BWI76_RS03660 and BWI76_RS03665 overlap by 4 nucleotides
BWI76_RS03645: BWI76_RS03645 - hypothetical protein, at 753,023 to 753,934
_RS03645
BWI76_RS03650: BWI76_RS03650 - endoribonuclease SymE, at 754,386 to 754,721
_RS03650
BWI76_RS03655: BWI76_RS03655 - hypothetical protein, at 754,720 to 754,920
_RS03655
BWI76_RS03660: BWI76_RS03660 - hypothetical protein, at 754,892 to 756,757
_RS03660
BWI76_RS03665: BWI76_RS03665 - ABC transporter ATP-binding protein, at 756,754 to 758,490
_RS03665
Position (kb) 754
755
756
757 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 753.893 kb on - strand at 753.902 kb on + strand at 753.903 kb on + strand at 753.903 kb on + strand at 753.903 kb on + strand at 753.903 kb on + strand at 753.903 kb on + strand at 753.903 kb on + strand at 753.910 kb on - strand at 753.910 kb on - strand at 753.910 kb on - strand at 753.910 kb on - strand at 753.911 kb on - strand at 753.911 kb on - strand at 753.911 kb on - strand at 753.911 kb on - strand at 753.911 kb on - strand at 753.911 kb on - strand at 753.911 kb on - strand at 753.911 kb on - strand at 753.911 kb on - strand at 753.937 kb on + strand at 753.954 kb on - strand at 753.956 kb on - strand at 753.987 kb on + strand at 754.018 kb on - strand at 754.093 kb on - strand at 754.170 kb on + strand at 754.177 kb on + strand at 754.177 kb on + strand at 754.177 kb on + strand at 754.185 kb on + strand at 754.198 kb on + strand at 754.198 kb on + strand at 754.203 kb on + strand at 754.211 kb on + strand at 754.219 kb on - strand at 754.219 kb on - strand at 754.243 kb on + strand at 754.243 kb on + strand at 754.258 kb on - strand at 754.261 kb on + strand at 754.261 kb on + strand at 754.261 kb on + strand at 754.261 kb on + strand at 754.261 kb on + strand at 754.261 kb on + strand at 754.261 kb on + strand at 754.261 kb on + strand at 754.261 kb on + strand at 754.261 kb on + strand at 754.261 kb on + strand at 754.261 kb on + strand at 754.261 kb on + strand at 754.261 kb on + strand at 754.261 kb on + strand at 754.261 kb on + strand at 754.261 kb on + strand at 754.262 kb on + strand at 754.262 kb on + strand at 754.262 kb on - strand at 754.269 kb on - strand at 754.269 kb on - strand at 754.269 kb on - strand at 754.269 kb on - strand at 754.269 kb on - strand at 754.270 kb on - strand at 754.276 kb on - strand at 754.288 kb on + strand at 754.303 kb on + strand at 754.313 kb on + strand at 754.320 kb on - strand at 754.320 kb on - strand at 754.320 kb on - strand at 754.333 kb on + strand at 754.351 kb on + strand at 754.351 kb on + strand at 754.351 kb on + strand at 754.351 kb on + strand at 754.354 kb on - strand at 754.354 kb on - strand at 754.356 kb on + strand at 754.359 kb on - strand at 754.359 kb on - strand at 754.359 kb on - strand at 754.361 kb on + strand at 754.361 kb on + strand at 754.361 kb on + strand at 754.361 kb on + strand at 754.364 kb on - strand at 754.364 kb on - strand at 754.392 kb on - strand at 754.394 kb on + strand at 754.394 kb on + strand at 754.400 kb on - strand at 754.402 kb on - strand at 754.402 kb on - strand at 754.402 kb on - strand at 754.402 kb on - strand at 754.407 kb on - strand at 754.422 kb on + strand, within BWI76_RS03650 at 754.422 kb on + strand, within BWI76_RS03650 at 754.430 kb on - strand, within BWI76_RS03650 at 754.430 kb on - strand, within BWI76_RS03650 at 754.430 kb on - strand, within BWI76_RS03650 at 754.430 kb on - strand, within BWI76_RS03650 at 754.430 kb on - strand, within BWI76_RS03650 at 754.439 kb on + strand, within BWI76_RS03650 at 754.439 kb on + strand, within BWI76_RS03650 at 754.488 kb on - strand, within BWI76_RS03650 at 754.488 kb on - strand, within BWI76_RS03650 at 754.498 kb on - strand, within BWI76_RS03650 at 754.520 kb on + strand, within BWI76_RS03650 at 754.520 kb on + strand, within BWI76_RS03650 at 754.520 kb on + strand, within BWI76_RS03650 at 754.523 kb on - strand, within BWI76_RS03650 at 754.523 kb on - strand, within BWI76_RS03650 at 754.523 kb on - strand, within BWI76_RS03650 at 754.527 kb on + strand, within BWI76_RS03650 at 754.528 kb on - strand, within BWI76_RS03650 at 754.528 kb on - strand, within BWI76_RS03650 at 754.528 kb on - strand, within BWI76_RS03650 at 754.535 kb on - strand, within BWI76_RS03650 at 754.610 kb on + strand, within BWI76_RS03650 at 754.632 kb on - strand, within BWI76_RS03650 at 754.632 kb on - strand, within BWI76_RS03650 at 754.649 kb on - strand, within BWI76_RS03650 at 754.655 kb on - strand, within BWI76_RS03650 at 754.679 kb on + strand, within BWI76_RS03650 at 754.679 kb on + strand, within BWI76_RS03650 at 754.679 kb on + strand, within BWI76_RS03650 at 754.687 kb on - strand, within BWI76_RS03650 at 754.687 kb on - strand, within BWI76_RS03650 at 754.687 kb on - strand, within BWI76_RS03650 at 754.687 kb on - strand, within BWI76_RS03650 at 754.715 kb on - strand at 754.717 kb on + strand at 754.725 kb on - strand at 754.733 kb on - strand at 754.755 kb on - strand, within BWI76_RS03655 at 754.755 kb on - strand, within BWI76_RS03655 at 754.768 kb on + strand, within BWI76_RS03655 at 754.769 kb on + strand, within BWI76_RS03655 at 754.770 kb on - strand, within BWI76_RS03655 at 754.776 kb on - strand, within BWI76_RS03655 at 754.810 kb on - strand, within BWI76_RS03655 at 754.861 kb on + strand, within BWI76_RS03655 at 754.874 kb on - strand, within BWI76_RS03655 at 754.876 kb on - strand, within BWI76_RS03655 at 754.877 kb on + strand, within BWI76_RS03655 at 754.948 kb on + strand at 755.084 kb on - strand, within BWI76_RS03660 at 755.185 kb on + strand, within BWI76_RS03660 at 755.435 kb on - strand, within BWI76_RS03660 at 756.027 kb on + strand, within BWI76_RS03660 at 756.084 kb on + strand, within BWI76_RS03660 at 756.159 kb on + strand, within BWI76_RS03660 at 756.299 kb on + strand, within BWI76_RS03660 at 756.337 kb on + strand, within BWI76_RS03660 at 756.337 kb on + strand, within BWI76_RS03660 at 756.863 kb on - strand at 757.161 kb on + strand, within BWI76_RS03665
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 753,893 - +1.3 753,902 + -0.3 753,903 + -0.5 753,903 + +0.5 753,903 + +0.4 753,903 + -0.3 753,903 + +2.3 753,903 + -1.7 753,910 - -0.4 753,910 - +1.1 753,910 - -0.2 753,910 - +0.3 753,911 - -0.2 753,911 - +0.0 753,911 - +1.8 753,911 - +2.3 753,911 - +1.3 753,911 - +0.1 753,911 - +1.1 753,911 - +0.7 753,911 - +1.2 753,937 + -1.1 753,954 - -0.4 753,956 - -0.1 753,987 + -2.6 754,018 - -0.7 754,093 - -1.2 754,170 + +0.7 754,177 + -2.9 754,177 + +0.8 754,177 + -1.4 754,185 + +0.9 754,198 + -0.8 754,198 + +2.0 754,203 + +0.1 754,211 + +1.5 754,219 - +0.3 754,219 - -3.1 754,243 + -0.7 754,243 + -0.1 754,258 - -0.2 754,261 + +0.2 754,261 + +0.3 754,261 + +0.1 754,261 + -0.7 754,261 + +0.3 754,261 + +0.6 754,261 + -1.3 754,261 + +0.2 754,261 + -0.1 754,261 + +0.1 754,261 + +1.3 754,261 + +1.3 754,261 + -0.3 754,261 + +0.1 754,261 + +0.4 754,261 + +1.2 754,261 + -0.3 754,262 + +2.8 754,262 + -0.3 754,262 - +1.3 754,269 - +0.2 754,269 - +0.7 754,269 - +1.3 754,269 - +1.1 754,269 - -0.3 754,270 - -0.6 754,276 - +2.0 754,288 + +0.3 754,303 + -0.7 754,313 + +0.4 754,320 - -2.5 754,320 - -0.2 754,320 - +0.3 754,333 + +0.9 754,351 + -2.3 754,351 + +0.0 754,351 + +1.0 754,351 + -2.0 754,354 - +0.0 754,354 - +0.8 754,356 + -1.2 754,359 - -1.1 754,359 - -0.2 754,359 - -0.7 754,361 + +0.5 754,361 + -0.5 754,361 + +0.1 754,361 + -1.1 754,364 - -0.5 754,364 - +0.8 754,392 - -2.4 754,394 + -0.7 754,394 + -0.9 754,400 - +1.1 754,402 - +0.0 754,402 - -1.2 754,402 - -0.5 754,402 - -0.1 754,407 - +2.3 754,422 + BWI76_RS03650 0.11 +0.3 754,422 + BWI76_RS03650 0.11 +0.1 754,430 - BWI76_RS03650 0.13 -1.7 754,430 - BWI76_RS03650 0.13 +0.1 754,430 - BWI76_RS03650 0.13 +1.3 754,430 - BWI76_RS03650 0.13 -0.8 754,430 - BWI76_RS03650 0.13 -0.8 754,439 + BWI76_RS03650 0.16 +0.3 754,439 + BWI76_RS03650 0.16 -0.2 754,488 - BWI76_RS03650 0.30 -3.1 754,488 - BWI76_RS03650 0.30 -0.7 754,498 - BWI76_RS03650 0.33 -2.2 754,520 + BWI76_RS03650 0.40 +0.7 754,520 + BWI76_RS03650 0.40 +0.6 754,520 + BWI76_RS03650 0.40 +0.7 754,523 - BWI76_RS03650 0.41 +0.9 754,523 - BWI76_RS03650 0.41 -0.8 754,523 - BWI76_RS03650 0.41 +1.3 754,527 + BWI76_RS03650 0.42 -1.6 754,528 - BWI76_RS03650 0.42 +0.2 754,528 - BWI76_RS03650 0.42 -0.9 754,528 - BWI76_RS03650 0.42 +0.3 754,535 - BWI76_RS03650 0.44 -0.5 754,610 + BWI76_RS03650 0.67 -0.0 754,632 - BWI76_RS03650 0.73 -0.4 754,632 - BWI76_RS03650 0.73 -1.9 754,649 - BWI76_RS03650 0.78 +0.2 754,655 - BWI76_RS03650 0.80 +0.6 754,679 + BWI76_RS03650 0.87 +0.7 754,679 + BWI76_RS03650 0.87 +0.4 754,679 + BWI76_RS03650 0.87 -1.3 754,687 - BWI76_RS03650 0.90 -1.6 754,687 - BWI76_RS03650 0.90 +0.1 754,687 - BWI76_RS03650 0.90 +0.1 754,687 - BWI76_RS03650 0.90 -0.4 754,715 - -1.9 754,717 + -0.2 754,725 - +0.4 754,733 - -0.5 754,755 - BWI76_RS03655 0.17 +0.1 754,755 - BWI76_RS03655 0.17 +1.2 754,768 + BWI76_RS03655 0.24 +1.3 754,769 + BWI76_RS03655 0.24 -2.6 754,770 - BWI76_RS03655 0.25 -0.7 754,776 - BWI76_RS03655 0.28 +0.6 754,810 - BWI76_RS03655 0.45 -1.6 754,861 + BWI76_RS03655 0.70 -1.6 754,874 - BWI76_RS03655 0.77 -0.1 754,876 - BWI76_RS03655 0.78 -0.5 754,877 + BWI76_RS03655 0.78 -0.7 754,948 + -2.2 755,084 - BWI76_RS03660 0.10 -0.3 755,185 + BWI76_RS03660 0.16 -1.3 755,435 - BWI76_RS03660 0.29 -1.1 756,027 + BWI76_RS03660 0.61 -3.0 756,084 + BWI76_RS03660 0.64 +0.3 756,159 + BWI76_RS03660 0.68 +0.3 756,299 + BWI76_RS03660 0.75 -1.0 756,337 + BWI76_RS03660 0.77 +0.3 756,337 + BWI76_RS03660 0.77 -0.7 756,863 - -0.3 757,161 + BWI76_RS03665 0.23 +0.9
Or see this region's nucleotide sequence