Experiment: RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03215 and BWI76_RS03220 are separated by 79 nucleotides BWI76_RS03220 and BWI76_RS03225 are separated by 429 nucleotides
BWI76_RS03215: BWI76_RS03215 - hypothetical protein, at 662,631 to 663,731
_RS03215
BWI76_RS03220: BWI76_RS03220 - hypothetical protein, at 663,811 to 664,334
_RS03220
BWI76_RS03225: BWI76_RS03225 - transcriptional regulator, at 664,764 to 665,570
_RS03225
Position (kb) 663
664
665 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 662.820 kb on - strand, within BWI76_RS03215 at 662.865 kb on - strand, within BWI76_RS03215 at 662.943 kb on + strand, within BWI76_RS03215 at 662.951 kb on - strand, within BWI76_RS03215 at 662.960 kb on + strand, within BWI76_RS03215 at 663.081 kb on - strand, within BWI76_RS03215 at 663.081 kb on - strand, within BWI76_RS03215 at 663.082 kb on - strand, within BWI76_RS03215 at 663.093 kb on - strand, within BWI76_RS03215 at 663.177 kb on + strand, within BWI76_RS03215 at 663.184 kb on + strand, within BWI76_RS03215 at 663.359 kb on + strand, within BWI76_RS03215 at 663.434 kb on + strand, within BWI76_RS03215 at 663.465 kb on - strand at 663.536 kb on + strand, within BWI76_RS03215 at 663.536 kb on + strand, within BWI76_RS03215 at 663.536 kb on + strand, within BWI76_RS03215 at 663.536 kb on + strand, within BWI76_RS03215 at 663.536 kb on + strand, within BWI76_RS03215 at 663.536 kb on + strand, within BWI76_RS03215 at 663.544 kb on - strand, within BWI76_RS03215 at 663.544 kb on - strand, within BWI76_RS03215 at 663.626 kb on + strand at 663.715 kb on + strand at 663.726 kb on + strand at 663.755 kb on - strand at 663.771 kb on + strand at 663.816 kb on - strand at 663.823 kb on + strand at 663.834 kb on - strand at 663.849 kb on + strand at 663.854 kb on + strand at 663.857 kb on - strand at 663.860 kb on + strand at 663.867 kb on + strand, within BWI76_RS03220 at 663.867 kb on + strand at 663.867 kb on - strand, within BWI76_RS03220 at 663.867 kb on - strand, within BWI76_RS03220 at 663.867 kb on - strand, within BWI76_RS03220 at 663.875 kb on - strand, within BWI76_RS03220 at 663.875 kb on - strand, within BWI76_RS03220 at 663.875 kb on - strand, within BWI76_RS03220 at 663.876 kb on - strand, within BWI76_RS03220 at 663.876 kb on - strand, within BWI76_RS03220 at 663.928 kb on + strand, within BWI76_RS03220 at 663.936 kb on - strand, within BWI76_RS03220 at 663.962 kb on - strand, within BWI76_RS03220 at 663.970 kb on - strand, within BWI76_RS03220 at 663.972 kb on - strand, within BWI76_RS03220 at 664.033 kb on + strand, within BWI76_RS03220 at 664.033 kb on + strand, within BWI76_RS03220 at 664.043 kb on - strand, within BWI76_RS03220 at 664.087 kb on + strand, within BWI76_RS03220 at 664.087 kb on + strand, within BWI76_RS03220 at 664.102 kb on + strand, within BWI76_RS03220 at 664.107 kb on - strand, within BWI76_RS03220 at 664.112 kb on + strand, within BWI76_RS03220 at 664.120 kb on + strand, within BWI76_RS03220 at 664.120 kb on - strand, within BWI76_RS03220 at 664.121 kb on + strand, within BWI76_RS03220 at 664.128 kb on - strand, within BWI76_RS03220 at 664.129 kb on - strand, within BWI76_RS03220 at 664.129 kb on - strand, within BWI76_RS03220 at 664.131 kb on + strand, within BWI76_RS03220 at 664.139 kb on - strand, within BWI76_RS03220 at 664.139 kb on - strand, within BWI76_RS03220 at 664.141 kb on + strand, within BWI76_RS03220 at 664.168 kb on - strand, within BWI76_RS03220 at 664.168 kb on - strand, within BWI76_RS03220 at 664.177 kb on + strand, within BWI76_RS03220 at 664.177 kb on + strand, within BWI76_RS03220 at 664.184 kb on + strand, within BWI76_RS03220 at 664.184 kb on + strand, within BWI76_RS03220 at 664.184 kb on + strand, within BWI76_RS03220 at 664.192 kb on - strand, within BWI76_RS03220 at 664.192 kb on - strand, within BWI76_RS03220 at 664.192 kb on - strand, within BWI76_RS03220 at 664.192 kb on - strand, within BWI76_RS03220 at 664.231 kb on - strand, within BWI76_RS03220 at 664.247 kb on + strand, within BWI76_RS03220 at 664.252 kb on - strand, within BWI76_RS03220 at 664.255 kb on - strand, within BWI76_RS03220 at 664.255 kb on - strand, within BWI76_RS03220 at 664.273 kb on + strand, within BWI76_RS03220 at 664.277 kb on - strand, within BWI76_RS03220 at 664.277 kb on - strand, within BWI76_RS03220 at 664.279 kb on + strand, within BWI76_RS03220 at 664.282 kb on - strand at 664.291 kb on - strand at 664.293 kb on - strand at 664.293 kb on - strand at 664.293 kb on - strand at 664.306 kb on - strand at 664.341 kb on + strand at 664.341 kb on + strand at 664.342 kb on + strand at 664.348 kb on + strand at 664.348 kb on + strand at 664.348 kb on + strand at 664.348 kb on - strand at 664.348 kb on - strand at 664.349 kb on - strand at 664.349 kb on - strand at 664.350 kb on - strand at 664.356 kb on + strand at 664.356 kb on - strand at 664.357 kb on - strand at 664.364 kb on - strand at 664.364 kb on - strand at 664.370 kb on + strand at 664.370 kb on - strand at 664.372 kb on + strand at 664.372 kb on + strand at 664.372 kb on + strand at 664.372 kb on + strand at 664.372 kb on + strand at 664.372 kb on + strand at 664.380 kb on - strand at 664.380 kb on - strand at 664.380 kb on - strand at 664.393 kb on + strand at 664.426 kb on + strand at 664.434 kb on - strand at 664.434 kb on - strand at 664.435 kb on - strand at 664.447 kb on + strand at 664.457 kb on + strand at 664.457 kb on + strand at 664.462 kb on + strand at 664.462 kb on + strand at 664.462 kb on + strand at 664.462 kb on + strand at 664.462 kb on + strand at 664.462 kb on + strand at 664.462 kb on + strand at 664.462 kb on + strand at 664.462 kb on + strand at 664.462 kb on + strand at 664.462 kb on + strand at 664.462 kb on + strand at 664.462 kb on + strand at 664.462 kb on + strand at 664.462 kb on + strand at 664.462 kb on + strand at 664.462 kb on + strand at 664.462 kb on + strand at 664.462 kb on + strand at 664.462 kb on + strand at 664.464 kb on + strand at 664.464 kb on + strand at 664.464 kb on + strand at 664.464 kb on + strand at 664.465 kb on - strand at 664.465 kb on - strand at 664.470 kb on - strand at 664.470 kb on - strand at 664.470 kb on - strand at 664.470 kb on - strand at 664.470 kb on - strand at 664.470 kb on - strand at 664.470 kb on - strand at 664.470 kb on - strand at 664.470 kb on - strand at 664.470 kb on - strand at 664.470 kb on - strand at 664.470 kb on - strand at 664.470 kb on - strand at 664.470 kb on - strand at 664.470 kb on - strand at 664.470 kb on - strand at 664.470 kb on - strand at 664.470 kb on - strand at 664.470 kb on - strand at 664.470 kb on - strand at 664.470 kb on - strand at 664.470 kb on - strand at 664.470 kb on - strand at 664.470 kb on - strand at 664.470 kb on - strand at 664.470 kb on - strand at 664.472 kb on - strand at 664.472 kb on - strand at 664.472 kb on - strand at 664.472 kb on - strand at 664.472 kb on - strand at 664.472 kb on - strand at 664.472 kb on - strand at 664.472 kb on - strand at 664.472 kb on - strand at 664.472 kb on - strand at 664.472 kb on - strand at 664.478 kb on - strand at 664.478 kb on - strand at 664.478 kb on - strand at 664.478 kb on - strand at 664.478 kb on - strand at 664.480 kb on + strand at 664.480 kb on + strand at 664.480 kb on + strand at 664.480 kb on + strand at 664.480 kb on + strand at 664.480 kb on + strand at 664.480 kb on + strand at 664.480 kb on + strand at 664.480 kb on + strand at 664.480 kb on + strand at 664.480 kb on + strand at 664.480 kb on + strand at 664.488 kb on - strand at 664.488 kb on - strand at 664.488 kb on - strand at 664.488 kb on - strand at 664.488 kb on - strand at 664.488 kb on - strand at 664.488 kb on - strand at 664.488 kb on - strand at 664.488 kb on - strand at 664.488 kb on - strand at 664.488 kb on - strand at 664.488 kb on - strand at 664.488 kb on - strand at 664.488 kb on - strand at 664.488 kb on - strand at 664.488 kb on - strand at 664.488 kb on - strand at 664.488 kb on - strand at 664.488 kb on - strand at 664.488 kb on - strand at 664.488 kb on - strand at 664.488 kb on - strand at 664.488 kb on - strand at 664.488 kb on - strand at 664.488 kb on - strand at 664.488 kb on - strand at 664.488 kb on - strand at 664.488 kb on - strand at 664.488 kb on - strand at 664.488 kb on - strand at 664.488 kb on - strand at 664.497 kb on + strand at 664.505 kb on - strand at 664.507 kb on - strand at 664.507 kb on - strand at 664.520 kb on + strand at 664.530 kb on + strand at 664.533 kb on - strand at 664.538 kb on - strand at 664.538 kb on - strand at 664.538 kb on - strand at 664.555 kb on + strand at 664.555 kb on + strand at 664.563 kb on + strand at 664.563 kb on + strand at 664.566 kb on - strand at 664.570 kb on + strand at 664.570 kb on + strand at 664.570 kb on + strand at 664.570 kb on + strand at 664.570 kb on + strand at 664.570 kb on + strand at 664.570 kb on + strand at 664.570 kb on + strand at 664.570 kb on + strand at 664.570 kb on + strand at 664.570 kb on + strand at 664.570 kb on + strand at 664.572 kb on + strand at 664.572 kb on + strand at 664.578 kb on + strand at 664.578 kb on + strand at 664.578 kb on - strand at 664.578 kb on - strand at 664.578 kb on - strand at 664.578 kb on - strand at 664.578 kb on - strand at 664.578 kb on - strand at 664.578 kb on - strand at 664.578 kb on - strand at 664.578 kb on - strand at 664.578 kb on - strand at 664.578 kb on - strand at 664.578 kb on - strand at 664.580 kb on + strand at 664.580 kb on + strand at 664.588 kb on - strand at 664.588 kb on - strand at 664.588 kb on - strand at 664.588 kb on - strand at 664.588 kb on - strand at 664.590 kb on + strand at 664.593 kb on - strand at 664.601 kb on + strand at 664.601 kb on + strand at 664.602 kb on + strand at 664.602 kb on + strand at 664.607 kb on + strand at 664.608 kb on + strand at 664.609 kb on + strand at 664.609 kb on + strand at 664.609 kb on - strand at 664.609 kb on - strand at 664.609 kb on - strand at 664.610 kb on - strand at 664.610 kb on - strand at 664.611 kb on - strand at 664.615 kb on - strand at 664.616 kb on - strand at 664.616 kb on - strand at 664.617 kb on - strand at 664.617 kb on - strand at 664.617 kb on - strand at 664.617 kb on - strand at 664.617 kb on - strand at 664.627 kb on - strand at 664.649 kb on + strand at 664.649 kb on + strand at 664.658 kb on + strand at 664.660 kb on + strand at 664.660 kb on + strand at 664.660 kb on + strand at 664.660 kb on + strand at 664.660 kb on + strand at 664.660 kb on + strand at 664.660 kb on + strand at 664.660 kb on + strand at 664.660 kb on + strand at 664.660 kb on + strand at 664.666 kb on + strand at 664.668 kb on + strand at 664.668 kb on + strand at 664.668 kb on + strand at 664.668 kb on - strand at 664.668 kb on - strand at 664.668 kb on - strand at 664.668 kb on - strand at 664.668 kb on - strand at 664.668 kb on - strand at 664.668 kb on - strand at 664.668 kb on - strand at 664.668 kb on - strand at 664.668 kb on - strand at 664.668 kb on - strand at 664.668 kb on - strand at 664.668 kb on - strand at 664.668 kb on - strand at 664.668 kb on - strand at 664.668 kb on - strand at 664.671 kb on + strand at 664.679 kb on - strand at 664.679 kb on - strand at 664.681 kb on + strand at 664.690 kb on + strand at 664.696 kb on - strand at 664.700 kb on + strand at 664.719 kb on + strand at 664.738 kb on + strand at 664.738 kb on + strand at 664.748 kb on - strand at 664.748 kb on - strand at 664.793 kb on - strand at 664.795 kb on + strand at 664.798 kb on - strand at 664.820 kb on + strand at 664.836 kb on - strand at 665.006 kb on - strand, within BWI76_RS03225 at 665.091 kb on + strand, within BWI76_RS03225 at 665.100 kb on + strand, within BWI76_RS03225 at 665.100 kb on + strand, within BWI76_RS03225 at 665.108 kb on - strand at 665.108 kb on - strand, within BWI76_RS03225 at 665.130 kb on - strand, within BWI76_RS03225 at 665.130 kb on - strand, within BWI76_RS03225 at 665.155 kb on - strand, within BWI76_RS03225 at 665.155 kb on - strand, within BWI76_RS03225 at 665.155 kb on - strand, within BWI76_RS03225 at 665.155 kb on - strand, within BWI76_RS03225 at 665.158 kb on + strand, within BWI76_RS03225 at 665.190 kb on - strand, within BWI76_RS03225 at 665.261 kb on - strand, within BWI76_RS03225 at 665.261 kb on - strand, within BWI76_RS03225 at 665.275 kb on + strand, within BWI76_RS03225 at 665.279 kb on - strand, within BWI76_RS03225 at 665.307 kb on - strand, within BWI76_RS03225
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 662,820 - BWI76_RS03215 0.17 -2.2 662,865 - BWI76_RS03215 0.21 +2.4 662,943 + BWI76_RS03215 0.28 +0.1 662,951 - BWI76_RS03215 0.29 -0.1 662,960 + BWI76_RS03215 0.30 +0.6 663,081 - BWI76_RS03215 0.41 -1.1 663,081 - BWI76_RS03215 0.41 +1.8 663,082 - BWI76_RS03215 0.41 -1.9 663,093 - BWI76_RS03215 0.42 +1.1 663,177 + BWI76_RS03215 0.50 -0.8 663,184 + BWI76_RS03215 0.50 -0.4 663,359 + BWI76_RS03215 0.66 +1.3 663,434 + BWI76_RS03215 0.73 -0.5 663,465 - +0.0 663,536 + BWI76_RS03215 0.82 +0.3 663,536 + BWI76_RS03215 0.82 -3.4 663,536 + BWI76_RS03215 0.82 +0.1 663,536 + BWI76_RS03215 0.82 -0.7 663,536 + BWI76_RS03215 0.82 -0.7 663,536 + BWI76_RS03215 0.82 -1.3 663,544 - BWI76_RS03215 0.83 +3.3 663,544 - BWI76_RS03215 0.83 +0.8 663,626 + -0.1 663,715 + -0.1 663,726 + -0.1 663,755 - +0.1 663,771 + -0.4 663,816 - -1.0 663,823 + -0.3 663,834 - -0.3 663,849 + -0.5 663,854 + +1.7 663,857 - +0.3 663,860 + +2.3 663,867 + BWI76_RS03220 0.11 +1.0 663,867 + +0.3 663,867 - BWI76_RS03220 0.11 -0.6 663,867 - BWI76_RS03220 0.11 +0.8 663,867 - BWI76_RS03220 0.11 +0.4 663,875 - BWI76_RS03220 0.12 -0.3 663,875 - BWI76_RS03220 0.12 -0.0 663,875 - BWI76_RS03220 0.12 -0.0 663,876 - BWI76_RS03220 0.12 +0.4 663,876 - BWI76_RS03220 0.12 -0.4 663,928 + BWI76_RS03220 0.22 -0.6 663,936 - BWI76_RS03220 0.24 +0.4 663,962 - BWI76_RS03220 0.29 -1.0 663,970 - BWI76_RS03220 0.30 -0.4 663,972 - BWI76_RS03220 0.31 +0.4 664,033 + BWI76_RS03220 0.42 +0.1 664,033 + BWI76_RS03220 0.42 +0.5 664,043 - BWI76_RS03220 0.44 -1.3 664,087 + BWI76_RS03220 0.53 +3.1 664,087 + BWI76_RS03220 0.53 +0.6 664,102 + BWI76_RS03220 0.56 +0.3 664,107 - BWI76_RS03220 0.56 -1.0 664,112 + BWI76_RS03220 0.57 -0.3 664,120 + BWI76_RS03220 0.59 +0.3 664,120 - BWI76_RS03220 0.59 -2.3 664,121 + BWI76_RS03220 0.59 -1.1 664,128 - BWI76_RS03220 0.60 -1.9 664,129 - BWI76_RS03220 0.61 +2.6 664,129 - BWI76_RS03220 0.61 +0.3 664,131 + BWI76_RS03220 0.61 -0.6 664,139 - BWI76_RS03220 0.63 +0.0 664,139 - BWI76_RS03220 0.63 +0.4 664,141 + BWI76_RS03220 0.63 -1.1 664,168 - BWI76_RS03220 0.68 -0.4 664,168 - BWI76_RS03220 0.68 -0.6 664,177 + BWI76_RS03220 0.70 -0.5 664,177 + BWI76_RS03220 0.70 -0.3 664,184 + BWI76_RS03220 0.71 +1.3 664,184 + BWI76_RS03220 0.71 -0.1 664,184 + BWI76_RS03220 0.71 +0.1 664,192 - BWI76_RS03220 0.73 -1.5 664,192 - BWI76_RS03220 0.73 -1.2 664,192 - BWI76_RS03220 0.73 +0.5 664,192 - BWI76_RS03220 0.73 +0.2 664,231 - BWI76_RS03220 0.80 -2.2 664,247 + BWI76_RS03220 0.83 -0.5 664,252 - BWI76_RS03220 0.84 -0.6 664,255 - BWI76_RS03220 0.85 -0.3 664,255 - BWI76_RS03220 0.85 -0.4 664,273 + BWI76_RS03220 0.88 -2.7 664,277 - BWI76_RS03220 0.89 -0.4 664,277 - BWI76_RS03220 0.89 -3.5 664,279 + BWI76_RS03220 0.89 -1.3 664,282 - -0.4 664,291 - +0.5 664,293 - +1.0 664,293 - +1.3 664,293 - +0.2 664,306 - +0.9 664,341 + +2.1 664,341 + -0.3 664,342 + +0.3 664,348 + -1.5 664,348 + +0.5 664,348 + -1.9 664,348 - -0.8 664,348 - +1.2 664,349 - +0.1 664,349 - +0.1 664,350 - -1.4 664,356 + +0.9 664,356 - -1.1 664,357 - +1.1 664,364 - +0.4 664,364 - +0.8 664,370 + +1.3 664,370 - +0.8 664,372 + -0.2 664,372 + -0.6 664,372 + +0.3 664,372 + -2.7 664,372 + -2.1 664,372 + -2.7 664,380 - -1.5 664,380 - +0.1 664,380 - +0.1 664,393 + -1.7 664,426 + -2.2 664,434 - -0.9 664,434 - +0.7 664,435 - -0.2 664,447 + -0.8 664,457 + +3.1 664,457 + -0.2 664,462 + +1.3 664,462 + +0.3 664,462 + +1.3 664,462 + -0.2 664,462 + +1.1 664,462 + -3.2 664,462 + +1.3 664,462 + +2.6 664,462 + +0.6 664,462 + +0.0 664,462 + -0.7 664,462 + +1.3 664,462 + -0.7 664,462 + +0.2 664,462 + +1.1 664,462 + -0.7 664,462 + +1.1 664,462 + +0.3 664,462 + +2.4 664,462 + -0.7 664,464 + -1.1 664,464 + -3.0 664,464 + -2.5 664,464 + +0.8 664,465 - -1.9 664,465 - +0.5 664,470 - +0.1 664,470 - +0.4 664,470 - -0.3 664,470 - -4.0 664,470 - +0.5 664,470 - -0.3 664,470 - -1.1 664,470 - -2.2 664,470 - +0.8 664,470 - -0.9 664,470 - -0.2 664,470 - +1.4 664,470 - -2.1 664,470 - +2.3 664,470 - -0.5 664,470 - -0.4 664,470 - -1.9 664,470 - +0.8 664,470 - +0.9 664,470 - +1.2 664,470 - -0.3 664,470 - -1.7 664,470 - +2.9 664,470 - -1.1 664,470 - -0.9 664,470 - +0.3 664,472 - +0.0 664,472 - +0.3 664,472 - +1.3 664,472 - +0.2 664,472 - -0.9 664,472 - -0.1 664,472 - -1.3 664,472 - +0.3 664,472 - -0.8 664,472 - -2.4 664,472 - -2.6 664,478 - -0.5 664,478 - -3.6 664,478 - +0.3 664,478 - -0.1 664,478 - +0.4 664,480 + +0.3 664,480 + -1.4 664,480 + +1.5 664,480 + -2.5 664,480 + -1.7 664,480 + -0.7 664,480 + -0.3 664,480 + -2.1 664,480 + -0.9 664,480 + -1.7 664,480 + -0.3 664,480 + -1.7 664,488 - +1.8 664,488 - -0.3 664,488 - -0.7 664,488 - +0.0 664,488 - +1.3 664,488 - +0.4 664,488 - -1.1 664,488 - +2.1 664,488 - -2.1 664,488 - -1.1 664,488 - +0.1 664,488 - -0.1 664,488 - +0.8 664,488 - -0.3 664,488 - -0.3 664,488 - +1.3 664,488 - +0.4 664,488 - +0.2 664,488 - +0.5 664,488 - -2.1 664,488 - +1.7 664,488 - +1.1 664,488 - +0.3 664,488 - -0.7 664,488 - -2.3 664,488 - +1.5 664,488 - -1.1 664,488 - +0.3 664,488 - +1.7 664,488 - -0.0 664,488 - +0.5 664,497 + -0.3 664,505 - -0.1 664,507 - -1.3 664,507 - -1.0 664,520 + -0.3 664,530 + +1.3 664,533 - 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BWI76_RS03225 0.48 -0.3 665,155 - BWI76_RS03225 0.48 +1.4 665,155 - BWI76_RS03225 0.48 -2.1 665,155 - BWI76_RS03225 0.48 +1.3 665,158 + BWI76_RS03225 0.49 -0.7 665,190 - BWI76_RS03225 0.53 -2.9 665,261 - BWI76_RS03225 0.62 -1.9 665,261 - BWI76_RS03225 0.62 +2.7 665,275 + BWI76_RS03225 0.63 +0.7 665,279 - BWI76_RS03225 0.64 +1.3 665,307 - BWI76_RS03225 0.67 +1.3
Or see this region's nucleotide sequence