Experiment: RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00975 and BWI76_RS00980 are separated by 2 nucleotides BWI76_RS00980 and BWI76_RS00985 are separated by 58 nucleotides
BWI76_RS00975: BWI76_RS00975 - dihydroxy-acid dehydratase, at 198,294 to 200,144
_RS00975
BWI76_RS00980: BWI76_RS00980 - PLP-dependent threonine dehydratase, at 200,147 to 201,691
_RS00980
BWI76_RS00985: BWI76_RS00985 - transcriptional regulator IlvY, at 201,750 to 202,640
_RS00985
Position (kb) 200
201
202 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 199.226 kb on + strand, within BWI76_RS00975 at 199.226 kb on + strand, within BWI76_RS00975 at 199.226 kb on + strand, within BWI76_RS00975 at 199.226 kb on + strand, within BWI76_RS00975 at 199.234 kb on - strand, within BWI76_RS00975 at 199.234 kb on - strand, within BWI76_RS00975 at 199.740 kb on - strand, within BWI76_RS00975 at 199.806 kb on + strand, within BWI76_RS00975 at 199.877 kb on - strand, within BWI76_RS00975 at 199.960 kb on - strand at 200.009 kb on + strand at 200.062 kb on + strand at 200.070 kb on - strand at 200.192 kb on - strand at 200.207 kb on - strand at 200.207 kb on - strand at 200.229 kb on - strand at 200.244 kb on - strand at 200.325 kb on + strand, within BWI76_RS00980 at 200.325 kb on + strand, within BWI76_RS00980 at 200.325 kb on + strand, within BWI76_RS00980 at 200.333 kb on - strand, within BWI76_RS00980 at 200.333 kb on - strand, within BWI76_RS00980 at 200.333 kb on - strand, within BWI76_RS00980 at 200.333 kb on - strand, within BWI76_RS00980 at 200.333 kb on - strand, within BWI76_RS00980 at 200.333 kb on - strand, within BWI76_RS00980 at 200.333 kb on - strand, within BWI76_RS00980 at 200.333 kb on - strand, within BWI76_RS00980 at 200.333 kb on - strand, within BWI76_RS00980 at 200.333 kb on - strand, within BWI76_RS00980 at 200.558 kb on + strand, within BWI76_RS00980 at 200.652 kb on - strand, within BWI76_RS00980 at 200.792 kb on + strand, within BWI76_RS00980 at 200.845 kb on - strand, within BWI76_RS00980 at 200.845 kb on - strand, within BWI76_RS00980 at 200.869 kb on - strand, within BWI76_RS00980 at 200.929 kb on + strand, within BWI76_RS00980 at 201.380 kb on - strand, within BWI76_RS00980 at 201.454 kb on - strand at 201.505 kb on + strand, within BWI76_RS00980 at 201.565 kb on - strand at 201.629 kb on - strand at 201.918 kb on - strand, within BWI76_RS00985 at 201.928 kb on - strand, within BWI76_RS00985 at 201.974 kb on + strand, within BWI76_RS00985 at 202.030 kb on - strand, within BWI76_RS00985 at 202.043 kb on - strand, within BWI76_RS00985 at 202.101 kb on - strand, within BWI76_RS00985 at 202.114 kb on + strand, within BWI76_RS00985 at 202.114 kb on + strand, within BWI76_RS00985 at 202.177 kb on + strand, within BWI76_RS00985 at 202.264 kb on + strand, within BWI76_RS00985 at 202.444 kb on + strand, within BWI76_RS00985 at 202.515 kb on + strand, within BWI76_RS00985 at 202.560 kb on + strand at 202.610 kb on + strand at 202.625 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 199,226 + BWI76_RS00975 0.50 -2.8 199,226 + BWI76_RS00975 0.50 -0.7 199,226 + BWI76_RS00975 0.50 -1.9 199,226 + BWI76_RS00975 0.50 -1.7 199,234 - BWI76_RS00975 0.51 -2.9 199,234 - BWI76_RS00975 0.51 -1.2 199,740 - BWI76_RS00975 0.78 +1.3 199,806 + BWI76_RS00975 0.82 -1.5 199,877 - BWI76_RS00975 0.86 -2.0 199,960 - -3.4 200,009 + -2.2 200,062 + -3.2 200,070 - -2.9 200,192 - -0.3 200,207 - -2.4 200,207 - -1.8 200,229 - -1.9 200,244 - -1.0 200,325 + BWI76_RS00980 0.12 -2.8 200,325 + BWI76_RS00980 0.12 -1.0 200,325 + BWI76_RS00980 0.12 -5.0 200,333 - BWI76_RS00980 0.12 -3.3 200,333 - BWI76_RS00980 0.12 -0.9 200,333 - BWI76_RS00980 0.12 -4.1 200,333 - BWI76_RS00980 0.12 -0.9 200,333 - BWI76_RS00980 0.12 -1.5 200,333 - BWI76_RS00980 0.12 -3.4 200,333 - BWI76_RS00980 0.12 -0.2 200,333 - BWI76_RS00980 0.12 -2.4 200,333 - BWI76_RS00980 0.12 -1.7 200,333 - BWI76_RS00980 0.12 -3.0 200,558 + BWI76_RS00980 0.27 -4.4 200,652 - BWI76_RS00980 0.33 -0.3 200,792 + BWI76_RS00980 0.42 -2.5 200,845 - BWI76_RS00980 0.45 +1.3 200,845 - BWI76_RS00980 0.45 -2.6 200,869 - BWI76_RS00980 0.47 -4.3 200,929 + BWI76_RS00980 0.51 -4.4 201,380 - BWI76_RS00980 0.80 -1.3 201,454 - +0.3 201,505 + BWI76_RS00980 0.88 +0.8 201,565 - -1.8 201,629 - -1.3 201,918 - BWI76_RS00985 0.19 -2.7 201,928 - BWI76_RS00985 0.20 -0.3 201,974 + BWI76_RS00985 0.25 -1.0 202,030 - BWI76_RS00985 0.31 -2.4 202,043 - BWI76_RS00985 0.33 -0.5 202,101 - BWI76_RS00985 0.39 -0.6 202,114 + BWI76_RS00985 0.41 -0.3 202,114 + BWI76_RS00985 0.41 -0.7 202,177 + BWI76_RS00985 0.48 -0.9 202,264 + BWI76_RS00985 0.58 +0.1 202,444 + BWI76_RS00985 0.78 -0.1 202,515 + BWI76_RS00985 0.86 -1.0 202,560 + -0.3 202,610 + +0.3 202,625 - -1.5
Or see this region's nucleotide sequence