Experiment: RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00845 and BWI76_RS00850 are separated by 9 nucleotides BWI76_RS00850 and BWI76_RS00855 are separated by 77 nucleotides
BWI76_RS00845: BWI76_RS00845 - N-acetyl-gamma-glutamyl-phosphate reductase, at 174,921 to 175,925
_RS00845
BWI76_RS00850: BWI76_RS00850 - acetylglutamate kinase, at 175,935 to 176,708
_RS00850
BWI76_RS00855: BWI76_RS00855 - argininosuccinate lyase, at 176,786 to 178,159
_RS00855
Position (kb) 175
176
177 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 174.966 kb on + strand at 174.996 kb on + strand at 175.207 kb on + strand, within BWI76_RS00845 at 175.291 kb on + strand, within BWI76_RS00845 at 175.291 kb on + strand, within BWI76_RS00845 at 175.329 kb on - strand, within BWI76_RS00845 at 175.483 kb on - strand, within BWI76_RS00845 at 175.498 kb on - strand, within BWI76_RS00845 at 175.593 kb on + strand, within BWI76_RS00845 at 175.709 kb on - strand, within BWI76_RS00845 at 175.714 kb on - strand, within BWI76_RS00845 at 175.739 kb on + strand, within BWI76_RS00845 at 175.775 kb on - strand, within BWI76_RS00845 at 175.803 kb on + strand, within BWI76_RS00845 at 175.825 kb on - strand at 176.129 kb on + strand, within BWI76_RS00850 at 176.171 kb on + strand, within BWI76_RS00850 at 176.235 kb on - strand, within BWI76_RS00850 at 176.263 kb on + strand, within BWI76_RS00850 at 176.350 kb on + strand, within BWI76_RS00850 at 176.350 kb on + strand, within BWI76_RS00850 at 176.358 kb on - strand, within BWI76_RS00850 at 176.583 kb on - strand, within BWI76_RS00850 at 176.612 kb on - strand, within BWI76_RS00850 at 176.652 kb on + strand at 176.655 kb on - strand at 176.669 kb on + strand at 176.669 kb on + strand at 176.737 kb on + strand at 176.861 kb on + strand at 176.876 kb on + strand at 176.917 kb on + strand at 176.966 kb on + strand, within BWI76_RS00855 at 176.994 kb on + strand, within BWI76_RS00855 at 177.129 kb on - strand, within BWI76_RS00855 at 177.132 kb on + strand, within BWI76_RS00855 at 177.164 kb on + strand, within BWI76_RS00855 at 177.231 kb on + strand, within BWI76_RS00855 at 177.375 kb on + strand, within BWI76_RS00855 at 177.458 kb on + strand, within BWI76_RS00855 at 177.466 kb on - strand, within BWI76_RS00855 at 177.598 kb on + strand, within BWI76_RS00855
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 174,966 + -4.9 174,996 + -0.0 175,207 + BWI76_RS00845 0.28 -4.1 175,291 + BWI76_RS00845 0.37 -2.6 175,291 + BWI76_RS00845 0.37 +1.3 175,329 - BWI76_RS00845 0.41 -2.5 175,483 - BWI76_RS00845 0.56 -1.7 175,498 - BWI76_RS00845 0.57 -1.9 175,593 + BWI76_RS00845 0.67 -2.9 175,709 - BWI76_RS00845 0.78 -1.7 175,714 - BWI76_RS00845 0.79 -2.8 175,739 + BWI76_RS00845 0.81 -1.7 175,775 - BWI76_RS00845 0.85 -1.9 175,803 + BWI76_RS00845 0.88 -2.9 175,825 - -2.8 176,129 + BWI76_RS00850 0.25 +0.0 176,171 + BWI76_RS00850 0.30 -1.0 176,235 - BWI76_RS00850 0.39 -2.0 176,263 + BWI76_RS00850 0.42 -2.7 176,350 + BWI76_RS00850 0.54 -2.1 176,350 + BWI76_RS00850 0.54 -2.8 176,358 - BWI76_RS00850 0.55 -2.1 176,583 - BWI76_RS00850 0.84 -2.9 176,612 - BWI76_RS00850 0.87 -4.5 176,652 + +0.2 176,655 - -4.4 176,669 + -5.3 176,669 + -1.2 176,737 + +0.3 176,861 + -4.5 176,876 + -2.9 176,917 + -2.2 176,966 + BWI76_RS00855 0.13 -1.4 176,994 + BWI76_RS00855 0.15 -2.2 177,129 - BWI76_RS00855 0.25 -2.8 177,132 + BWI76_RS00855 0.25 -3.5 177,164 + BWI76_RS00855 0.28 -3.0 177,231 + BWI76_RS00855 0.32 -2.2 177,375 + BWI76_RS00855 0.43 -4.6 177,458 + BWI76_RS00855 0.49 -1.0 177,466 - BWI76_RS00855 0.49 -3.2 177,598 + BWI76_RS00855 0.59 -2.0
Or see this region's nucleotide sequence