Experiment: RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00635 and BWI76_RS00640 are separated by 54 nucleotides BWI76_RS00640 and BWI76_RS00645 overlap by 4 nucleotides BWI76_RS00645 and BWI76_RS00650 are separated by 148 nucleotides
BWI76_RS00635: BWI76_RS00635 - inulin fructotransferase (DFA-I-forming), at 129,269 to 130,633
_RS00635
BWI76_RS00640: BWI76_RS00640 - L-rhamnose mutarotase, at 130,688 to 131,002
_RS00640
BWI76_RS00645: BWI76_RS00645 - lactaldehyde reductase, at 130,999 to 132,147
_RS00645
BWI76_RS00650: BWI76_RS00650 - rhamnose ABC transporter permease component, at 132,296 to 133,300
_RS00650
Position (kb) 130
131
132
133 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 130.051 kb on - strand, within BWI76_RS00635 at 130.056 kb on + strand, within BWI76_RS00635 at 130.071 kb on + strand at 130.071 kb on + strand, within BWI76_RS00635 at 130.073 kb on + strand, within BWI76_RS00635 at 130.084 kb on - strand, within BWI76_RS00635 at 130.111 kb on + strand, within BWI76_RS00635 at 130.136 kb on + strand, within BWI76_RS00635 at 130.144 kb on - strand, within BWI76_RS00635 at 130.162 kb on - strand, within BWI76_RS00635 at 130.185 kb on + strand, within BWI76_RS00635 at 130.185 kb on + strand, within BWI76_RS00635 at 130.185 kb on + strand, within BWI76_RS00635 at 130.185 kb on + strand, within BWI76_RS00635 at 130.185 kb on + strand, within BWI76_RS00635 at 130.187 kb on + strand, within BWI76_RS00635 at 130.187 kb on + strand, within BWI76_RS00635 at 130.187 kb on + strand, within BWI76_RS00635 at 130.195 kb on - strand, within BWI76_RS00635 at 130.210 kb on + strand, within BWI76_RS00635 at 130.219 kb on - strand, within BWI76_RS00635 at 130.228 kb on + strand, within BWI76_RS00635 at 130.236 kb on + strand, within BWI76_RS00635 at 130.236 kb on + strand, within BWI76_RS00635 at 130.236 kb on + strand, within BWI76_RS00635 at 130.236 kb on + strand, within BWI76_RS00635 at 130.236 kb on + strand, within BWI76_RS00635 at 130.236 kb on - strand, within BWI76_RS00635 at 130.237 kb on - strand, within BWI76_RS00635 at 130.243 kb on - strand, within BWI76_RS00635 at 130.246 kb on + strand, within BWI76_RS00635 at 130.251 kb on + strand at 130.254 kb on - strand, within BWI76_RS00635 at 130.254 kb on - strand, within BWI76_RS00635 at 130.254 kb on - strand, within BWI76_RS00635 at 130.307 kb on - strand, within BWI76_RS00635 at 130.317 kb on - strand at 130.329 kb on + strand, within BWI76_RS00635 at 130.329 kb on + strand, within BWI76_RS00635 at 130.329 kb on + strand, within BWI76_RS00635 at 130.329 kb on + strand, within BWI76_RS00635 at 130.337 kb on - strand, within BWI76_RS00635 at 130.337 kb on - strand, within BWI76_RS00635 at 130.337 kb on - strand, within BWI76_RS00635 at 130.403 kb on + strand, within BWI76_RS00635 at 130.462 kb on + strand, within BWI76_RS00635 at 130.462 kb on + strand, within BWI76_RS00635 at 130.462 kb on + strand, within BWI76_RS00635 at 130.465 kb on - strand, within BWI76_RS00635 at 130.470 kb on - strand, within BWI76_RS00635 at 130.488 kb on + strand, within BWI76_RS00635 at 130.488 kb on + strand, within BWI76_RS00635 at 130.488 kb on + strand, within BWI76_RS00635 at 130.497 kb on + strand at 130.525 kb on + strand at 130.585 kb on - strand at 130.599 kb on + strand at 130.661 kb on + strand at 130.707 kb on + strand at 130.733 kb on - strand, within BWI76_RS00640 at 130.842 kb on - strand, within BWI76_RS00640 at 131.161 kb on - strand, within BWI76_RS00645 at 131.192 kb on - strand, within BWI76_RS00645 at 131.386 kb on + strand, within BWI76_RS00645 at 131.386 kb on + strand, within BWI76_RS00645 at 131.394 kb on - strand, within BWI76_RS00645 at 131.512 kb on - strand, within BWI76_RS00645 at 131.555 kb on - strand, within BWI76_RS00645 at 131.563 kb on - strand, within BWI76_RS00645 at 131.674 kb on + strand, within BWI76_RS00645 at 131.792 kb on - strand, within BWI76_RS00645 at 131.945 kb on - strand, within BWI76_RS00645 at 132.025 kb on - strand, within BWI76_RS00645 at 132.136 kb on + strand at 132.186 kb on + strand at 132.266 kb on - strand at 132.523 kb on + strand, within BWI76_RS00650 at 132.619 kb on + strand, within BWI76_RS00650 at 132.684 kb on + strand, within BWI76_RS00650 at 132.786 kb on - strand, within BWI76_RS00650 at 132.791 kb on - strand, within BWI76_RS00650 at 132.832 kb on + strand, within BWI76_RS00650 at 132.897 kb on + strand, within BWI76_RS00650 at 132.907 kb on + strand, within BWI76_RS00650 at 132.947 kb on + strand, within BWI76_RS00650 at 133.029 kb on - strand, within BWI76_RS00650
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 130,051 - BWI76_RS00635 0.57 +0.2 130,056 + BWI76_RS00635 0.58 +0.5 130,071 + +1.3 130,071 + BWI76_RS00635 0.59 +0.3 130,073 + BWI76_RS00635 0.59 +0.5 130,084 - BWI76_RS00635 0.60 +1.5 130,111 + BWI76_RS00635 0.62 +0.7 130,136 + BWI76_RS00635 0.64 -0.5 130,144 - BWI76_RS00635 0.64 -0.7 130,162 - BWI76_RS00635 0.65 +1.9 130,185 + BWI76_RS00635 0.67 -3.4 130,185 + BWI76_RS00635 0.67 +1.3 130,185 + BWI76_RS00635 0.67 +1.1 130,185 + BWI76_RS00635 0.67 +0.8 130,185 + BWI76_RS00635 0.67 +1.3 130,187 + BWI76_RS00635 0.67 +1.0 130,187 + BWI76_RS00635 0.67 -0.4 130,187 + BWI76_RS00635 0.67 -0.1 130,195 - BWI76_RS00635 0.68 -0.1 130,210 + BWI76_RS00635 0.69 +0.9 130,219 - BWI76_RS00635 0.70 -0.7 130,228 + BWI76_RS00635 0.70 -0.7 130,236 + BWI76_RS00635 0.71 +0.8 130,236 + BWI76_RS00635 0.71 -2.5 130,236 + BWI76_RS00635 0.71 -1.7 130,236 + BWI76_RS00635 0.71 -1.3 130,236 + BWI76_RS00635 0.71 +0.7 130,236 - BWI76_RS00635 0.71 -0.7 130,237 - BWI76_RS00635 0.71 -3.8 130,243 - BWI76_RS00635 0.71 -0.9 130,246 + BWI76_RS00635 0.72 +1.2 130,251 + +2.3 130,254 - BWI76_RS00635 0.72 -0.4 130,254 - BWI76_RS00635 0.72 +0.2 130,254 - BWI76_RS00635 0.72 -0.4 130,307 - BWI76_RS00635 0.76 +1.1 130,317 - -0.7 130,329 + BWI76_RS00635 0.78 +0.2 130,329 + BWI76_RS00635 0.78 +0.3 130,329 + BWI76_RS00635 0.78 +1.1 130,329 + BWI76_RS00635 0.78 -0.4 130,337 - BWI76_RS00635 0.78 -0.4 130,337 - BWI76_RS00635 0.78 +1.2 130,337 - BWI76_RS00635 0.78 +1.5 130,403 + BWI76_RS00635 0.83 +1.3 130,462 + BWI76_RS00635 0.87 +0.8 130,462 + BWI76_RS00635 0.87 +2.4 130,462 + BWI76_RS00635 0.87 -0.5 130,465 - BWI76_RS00635 0.88 -0.9 130,470 - BWI76_RS00635 0.88 +1.6 130,488 + BWI76_RS00635 0.89 -2.0 130,488 + BWI76_RS00635 0.89 -0.6 130,488 + BWI76_RS00635 0.89 -0.3 130,497 + +0.6 130,525 + +1.2 130,585 - -0.3 130,599 + +1.3 130,661 + -3.0 130,707 + -0.2 130,733 - BWI76_RS00640 0.14 -0.8 130,842 - BWI76_RS00640 0.49 +0.5 131,161 - BWI76_RS00645 0.14 +0.5 131,192 - BWI76_RS00645 0.17 -0.4 131,386 + BWI76_RS00645 0.34 -3.0 131,386 + BWI76_RS00645 0.34 -0.0 131,394 - BWI76_RS00645 0.34 -2.1 131,512 - BWI76_RS00645 0.45 -0.2 131,555 - BWI76_RS00645 0.48 +0.3 131,563 - BWI76_RS00645 0.49 -0.6 131,674 + BWI76_RS00645 0.59 -1.7 131,792 - BWI76_RS00645 0.69 -3.2 131,945 - BWI76_RS00645 0.82 -0.5 132,025 - BWI76_RS00645 0.89 -1.0 132,136 + -0.3 132,186 + -1.0 132,266 - -2.4 132,523 + BWI76_RS00650 0.23 -0.6 132,619 + BWI76_RS00650 0.32 -0.1 132,684 + BWI76_RS00650 0.39 +2.3 132,786 - BWI76_RS00650 0.49 +0.1 132,791 - BWI76_RS00650 0.49 -0.1 132,832 + BWI76_RS00650 0.53 +1.0 132,897 + BWI76_RS00650 0.60 +0.5 132,907 + BWI76_RS00650 0.61 +0.6 132,947 + BWI76_RS00650 0.65 +0.3 133,029 - BWI76_RS00650 0.73 +0.3
Or see this region's nucleotide sequence