Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS00345

Experiment: RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
71,232	-				+1.0
71,303	-				-3.8
71,317	-		BWI76_RS00340	0.11	+0.4
71,529	+		BWI76_RS00340	0.32	-0.2
71,637	-		BWI76_RS00340	0.43	+0.1
72,120	+				+0.4
72,151	+				+1.5
72,151	+				+0.4
72,151	+				-0.4
72,151	+				-1.9
72,151	+				+2.0
72,158	+				+0.3
72,159	-				-2.3
72,159	-				-2.2
72,159	-				-0.2
72,159	-				-2.2
72,159	-				-0.3
72,201	-				-0.7
72,307	+		BWI76_RS00345	0.13	-3.2
72,339	+		BWI76_RS00345	0.18	-0.7
72,510	-		BWI76_RS00345	0.45	+0.3
72,510	-		BWI76_RS00345	0.45	-1.3
72,592	-		BWI76_RS00345	0.58	-0.7
72,592	-		BWI76_RS00345	0.58	-1.2
72,661	-		BWI76_RS00345	0.70	-3.2
72,745	-		BWI76_RS00345	0.83	-0.7
72,822	+				-1.7
72,830	-				+1.3
72,832	-				-0.1
72,875	+				+0.9
72,875	+				+0.5
72,875	+				-1.3
72,883	-				+0.0
72,883	-				-1.7
72,883	-				-1.3
72,959	+				-1.5
72,959	+				-1.0
72,967	-				-0.0
73,004	+		BWI76_RS00350	0.12	-0.0
73,019	+		BWI76_RS00350	0.13	+1.0
73,034	-				+1.3
73,047	+		BWI76_RS00350	0.16	-0.0
73,047	-		BWI76_RS00350	0.16	-0.5
73,111	+		BWI76_RS00350	0.23	-0.6
73,111	+		BWI76_RS00350	0.23	-0.0
73,111	+		BWI76_RS00350	0.23	+0.3
73,111	+		BWI76_RS00350	0.23	-2.0
73,121	+		BWI76_RS00350	0.25	-1.3
73,129	-		BWI76_RS00350	0.25	+0.2
73,129	-		BWI76_RS00350	0.25	-0.1
73,129	-		BWI76_RS00350	0.25	+0.3
73,136	+		BWI76_RS00350	0.26	-0.6
73,152	+		BWI76_RS00350	0.28	+0.3
73,169	-		BWI76_RS00350	0.30	+0.4
73,221	+		BWI76_RS00350	0.36	-1.5
73,238	+		BWI76_RS00350	0.38	+0.2
73,250	-		BWI76_RS00350	0.39	-1.3
73,255	+		BWI76_RS00350	0.39	+0.7
73,256	-		BWI76_RS00350	0.40	+0.5
73,274	-		BWI76_RS00350	0.42	-2.0
73,274	-		BWI76_RS00350	0.42	+1.6
73,279	-		BWI76_RS00350	0.42	-0.0
73,286	-		BWI76_RS00350	0.43	-1.0
73,317	+		BWI76_RS00350	0.46	-0.7
73,340	+		BWI76_RS00350	0.49	+0.3
73,348	-		BWI76_RS00350	0.50	-1.0
73,392	+		BWI76_RS00350	0.55	-1.5
73,474	-		BWI76_RS00350	0.64	+0.3
73,491	+		BWI76_RS00350	0.66	+0.0
73,491	+		BWI76_RS00350	0.66	+1.1
73,499	-		BWI76_RS00350	0.67	-2.9
73,501	-		BWI76_RS00350	0.67	+0.3
73,520	-		BWI76_RS00350	0.69	-3.2
73,520	-		BWI76_RS00350	0.69	+0.7
73,520	-		BWI76_RS00350	0.69	+0.0
73,521	-		BWI76_RS00350	0.69	-0.1
73,566	+		BWI76_RS00350	0.74	-0.6
73,566	+		BWI76_RS00350	0.74	+0.7
73,574	-		BWI76_RS00350	0.75	-0.4
73,580	+		BWI76_RS00350	0.76	-0.7
73,588	-		BWI76_RS00350	0.76	+0.3
73,599	-		BWI76_RS00350	0.78	+0.0
73,600	-		BWI76_RS00350	0.78	-0.4
73,610	-		BWI76_RS00350	0.79	+0.7
73,619	-				+0.4
73,625	-		BWI76_RS00350	0.81	-3.0
73,627	-		BWI76_RS00350	0.81	+0.4
73,703	+		BWI76_RS00350	0.89	-0.7
73,754	+				+0.3
73,754	+				-0.6
73,762	-				+0.2
73,824	-				-2.8
73,837	+				-0.8
73,837	+				+0.6
73,845	-				+0.7
73,845	-				-1.0