Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS00080

Experiment: RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
14,759	+		BWI76_RS00070	0.86	+1.3
14,884	+				+1.3
14,922	-				-2.7
14,925	-				+0.5
14,925	-				+0.7
15,018	-				+1.3
15,089	+		BWI76_RS00075	0.10	+1.0
15,320	-		BWI76_RS00075	0.43	+1.0
15,361	+		BWI76_RS00075	0.49	-1.3
16,009	+		BWI76_RS00080	0.35	+0.8
16,225	+		BWI76_RS00080	0.59	+3.1
16,451	+		BWI76_RS00080	0.85	+0.9
16,461	+		BWI76_RS00080	0.86	-0.3
16,469	-		BWI76_RS00080	0.87	+0.1
16,514	-				-1.5
16,529	-				-1.0
16,601	-				-3.6
16,609	+				-1.0
16,616	+				+0.5
16,631	+		BWI76_RS00085	0.11	+0.2
16,631	+		BWI76_RS00085	0.11	+0.9
16,932	-		BWI76_RS00085	0.76	+0.0
17,020	-				-0.3
17,088	-				+0.3
17,176	+				-0.1
17,176	+				+0.1
17,176	+				+1.0
17,176	+				+1.3
17,184	-				-0.4
17,184	-				+0.4
17,214	-				+1.2
17,244	+				+0.8
17,252	-				-1.0
17,252	-				+0.3
17,498	-		BWI76_RS00090	0.24	-1.9
17,539	+		BWI76_RS00090	0.28	-0.3
17,546	+		BWI76_RS00090	0.28	+1.3