Experiment: KB with Harmalol 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_5011 and Psyr_5012 are separated by 32 nucleotides Psyr_5012 and Psyr_RNA78 are separated by 117 nucleotides Psyr_RNA78 and Psyr_RNA79 are separated by 108 nucleotides Psyr_RNA79 and Psyr_RNA80 are separated by 144 nucleotides
Psyr_5011: Psyr_5011 - Amino acid adenylation:Thioester reductase, at 5,943,655 to 5,947,101
_5011
Psyr_5012: Psyr_5012 - conserved hypothetical protein, at 5,947,134 to 5,947,613
_5012
Psyr_RNA78: Psyr_RNA78 - 5S ribosomal RNA, at 5,947,731 to 5,947,845
_RNA78
Psyr_RNA79: Psyr_RNA79 - 5S ribosomal RNA, at 5,947,954 to 5,948,068
_RNA79
Psyr_RNA80: Psyr_RNA80 - 23S ribosomal RNA, at 5,948,213 to 5,951,115
_RNA80
Position (kb) 5947
5948 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5946.826 kb on + strand at 5946.826 kb on + strand at 5946.943 kb on + strand at 5946.943 kb on + strand at 5946.944 kb on - strand at 5946.944 kb on - strand at 5946.944 kb on - strand at 5947.033 kb on + strand at 5947.107 kb on + strand at 5947.119 kb on + strand at 5947.119 kb on + strand at 5947.119 kb on + strand at 5947.119 kb on + strand at 5947.119 kb on + strand at 5947.119 kb on + strand at 5947.119 kb on + strand at 5947.120 kb on - strand at 5947.120 kb on - strand at 5947.279 kb on + strand, within Psyr_5012 at 5947.280 kb on - strand, within Psyr_5012 at 5947.398 kb on + strand, within Psyr_5012 at 5947.398 kb on + strand, within Psyr_5012 at 5947.398 kb on + strand, within Psyr_5012 at 5947.398 kb on + strand, within Psyr_5012 at 5947.398 kb on + strand, within Psyr_5012 at 5947.398 kb on + strand, within Psyr_5012 at 5947.398 kb on + strand, within Psyr_5012 at 5947.398 kb on + strand, within Psyr_5012 at 5947.398 kb on + strand, within Psyr_5012 at 5947.398 kb on + strand, within Psyr_5012 at 5947.398 kb on + strand, within Psyr_5012 at 5947.398 kb on + strand, within Psyr_5012 at 5947.398 kb on + strand, within Psyr_5012 at 5947.398 kb on + strand, within Psyr_5012 at 5947.398 kb on + strand, within Psyr_5012 at 5947.398 kb on + strand, within Psyr_5012 at 5947.398 kb on + strand, within Psyr_5012 at 5947.399 kb on - strand, within Psyr_5012 at 5947.399 kb on - strand, within Psyr_5012 at 5947.399 kb on - strand, within Psyr_5012 at 5947.399 kb on - strand, within Psyr_5012 at 5947.399 kb on - strand, within Psyr_5012 at 5947.399 kb on - strand, within Psyr_5012 at 5947.399 kb on - strand, within Psyr_5012 at 5947.399 kb on - strand, within Psyr_5012 at 5947.399 kb on - strand, within Psyr_5012 at 5947.399 kb on - strand, within Psyr_5012 at 5947.399 kb on - strand, within Psyr_5012 at 5947.399 kb on - strand, within Psyr_5012 at 5947.399 kb on - strand, within Psyr_5012 at 5947.399 kb on - strand, within Psyr_5012 at 5947.399 kb on - strand, within Psyr_5012 at 5947.399 kb on - strand, within Psyr_5012 at 5947.399 kb on - strand, within Psyr_5012 at 5947.399 kb on - strand, within Psyr_5012 at 5947.400 kb on + strand, within Psyr_5012 at 5947.400 kb on + strand, within Psyr_5012 at 5947.400 kb on + strand, within Psyr_5012 at 5947.400 kb on + strand, within Psyr_5012 at 5947.400 kb on + strand, within Psyr_5012 at 5947.400 kb on + strand, within Psyr_5012 at 5947.400 kb on + strand, within Psyr_5012 at 5947.400 kb on + strand, within Psyr_5012 at 5947.401 kb on - strand, within Psyr_5012 at 5947.401 kb on - strand, within Psyr_5012 at 5947.401 kb on - strand, within Psyr_5012 at 5947.401 kb on - strand, within Psyr_5012 at 5947.401 kb on - strand, within Psyr_5012 at 5947.401 kb on - strand, within Psyr_5012 at 5947.401 kb on - strand, within Psyr_5012 at 5947.401 kb on - strand, within Psyr_5012 at 5947.401 kb on - strand, within Psyr_5012 at 5947.401 kb on - strand, within Psyr_5012 at 5947.485 kb on - strand, within Psyr_5012 at 5947.590 kb on - strand at 5947.666 kb on + strand at 5947.666 kb on + strand at 5947.745 kb on - strand, within Psyr_RNA78 at 5947.745 kb on - strand, within Psyr_RNA78 at 5947.745 kb on - strand, within Psyr_RNA78 at 5947.745 kb on - strand, within Psyr_RNA78 at 5947.745 kb on - strand, within Psyr_RNA78 at 5947.745 kb on - strand, within Psyr_RNA78 at 5947.782 kb on + strand, within Psyr_RNA78 at 5947.833 kb on + strand, within Psyr_RNA78 at 5947.833 kb on + strand, within Psyr_RNA78 at 5947.834 kb on - strand at 5947.834 kb on - strand at 5947.874 kb on - strand at 5947.874 kb on - strand at 5947.901 kb on + strand at 5947.901 kb on + strand at 5947.902 kb on - strand at 5947.902 kb on - strand at 5947.902 kb on - strand at 5947.902 kb on - strand at 5947.902 kb on - strand at 5947.902 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Harmalol 10 mM remove 5,946,826 + -0.2 5,946,826 + -0.7 5,946,943 + -0.3 5,946,943 + +0.3 5,946,944 - -0.4 5,946,944 - -0.4 5,946,944 - +0.9 5,947,033 + -1.9 5,947,107 + -0.5 5,947,119 + -0.5 5,947,119 + +0.1 5,947,119 + +0.8 5,947,119 + -1.0 5,947,119 + -0.3 5,947,119 + +0.2 5,947,119 + +0.9 5,947,120 - -1.5 5,947,120 - +0.3 5,947,279 + Psyr_5012 0.30 -0.5 5,947,280 - Psyr_5012 0.30 -0.5 5,947,398 + Psyr_5012 0.55 +0.9 5,947,398 + Psyr_5012 0.55 -0.5 5,947,398 + Psyr_5012 0.55 +0.7 5,947,398 + Psyr_5012 0.55 +1.3 5,947,398 + Psyr_5012 0.55 +0.3 5,947,398 + Psyr_5012 0.55 +0.8 5,947,398 + Psyr_5012 0.55 +0.5 5,947,398 + Psyr_5012 0.55 -0.1 5,947,398 + Psyr_5012 0.55 -0.7 5,947,398 + Psyr_5012 0.55 -0.4 5,947,398 + Psyr_5012 0.55 -1.3 5,947,398 + Psyr_5012 0.55 -0.1 5,947,398 + Psyr_5012 0.55 +0.2 5,947,398 + Psyr_5012 0.55 +0.0 5,947,398 + Psyr_5012 0.55 +1.0 5,947,398 + Psyr_5012 0.55 +0.4 5,947,398 + Psyr_5012 0.55 +0.2 5,947,399 - Psyr_5012 0.55 +0.3 5,947,399 - Psyr_5012 0.55 +0.5 5,947,399 - Psyr_5012 0.55 +0.1 5,947,399 - Psyr_5012 0.55 +0.1 5,947,399 - Psyr_5012 0.55 +0.1 5,947,399 - Psyr_5012 0.55 +0.9 5,947,399 - Psyr_5012 0.55 +0.9 5,947,399 - Psyr_5012 0.55 -0.2 5,947,399 - Psyr_5012 0.55 +0.5 5,947,399 - Psyr_5012 0.55 -0.1 5,947,399 - Psyr_5012 0.55 +0.5 5,947,399 - Psyr_5012 0.55 -1.1 5,947,399 - Psyr_5012 0.55 -0.6 5,947,399 - Psyr_5012 0.55 +0.0 5,947,399 - Psyr_5012 0.55 +0.1 5,947,399 - Psyr_5012 0.55 -0.1 5,947,399 - Psyr_5012 0.55 +0.3 5,947,399 - Psyr_5012 0.55 +0.5 5,947,400 + Psyr_5012 0.55 +1.6 5,947,400 + Psyr_5012 0.55 +0.7 5,947,400 + Psyr_5012 0.55 -0.4 5,947,400 + Psyr_5012 0.55 +0.2 5,947,400 + Psyr_5012 0.55 -0.0 5,947,400 + Psyr_5012 0.55 -0.8 5,947,400 + Psyr_5012 0.55 +0.8 5,947,400 + Psyr_5012 0.55 +0.4 5,947,401 - Psyr_5012 0.56 -0.4 5,947,401 - Psyr_5012 0.56 +0.5 5,947,401 - Psyr_5012 0.56 +0.4 5,947,401 - Psyr_5012 0.56 +0.5 5,947,401 - Psyr_5012 0.56 -0.4 5,947,401 - Psyr_5012 0.56 +0.2 5,947,401 - Psyr_5012 0.56 -0.8 5,947,401 - Psyr_5012 0.56 +0.1 5,947,401 - Psyr_5012 0.56 -0.3 5,947,401 - Psyr_5012 0.56 +1.2 5,947,485 - Psyr_5012 0.73 +0.6 5,947,590 - -1.5 5,947,666 + +0.2 5,947,666 + +0.0 5,947,745 - Psyr_RNA78 0.12 -0.2 5,947,745 - Psyr_RNA78 0.12 -0.1 5,947,745 - Psyr_RNA78 0.12 +0.4 5,947,745 - Psyr_RNA78 0.12 -1.6 5,947,745 - Psyr_RNA78 0.12 -0.5 5,947,745 - Psyr_RNA78 0.12 -1.0 5,947,782 + Psyr_RNA78 0.44 -1.6 5,947,833 + Psyr_RNA78 0.89 -2.4 5,947,833 + Psyr_RNA78 0.89 -0.1 5,947,834 - +0.0 5,947,834 - +0.9 5,947,874 - -0.9 5,947,874 - -0.0 5,947,901 + -2.0 5,947,901 + -1.6 5,947,902 - +0.0 5,947,902 - +1.5 5,947,902 - -0.1 5,947,902 - +1.7 5,947,902 - +1.8 5,947,902 - +0.9
Or see this region's nucleotide sequence