Experiment: KB with Harmalol 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3807 and Psyr_3808 overlap by 4 nucleotides Psyr_3808 and Psyr_3809 overlap by 4 nucleotides Psyr_3809 and Psyr_3810 are separated by 142 nucleotides
Psyr_3807: Psyr_3807 - hypothetical protein, at 4,527,902 to 4,529,116
_3807
Psyr_3808: Psyr_3808 - hypothetical protein, at 4,529,113 to 4,529,592
_3808
Psyr_3809: Psyr_3809 - hypothetical protein, at 4,529,589 to 4,531,439
_3809
Psyr_3810: Psyr_3810 - Integrase, catalytic region, at 4,531,582 to 4,532,403
_3810
Position (kb) 4529
4530
4531
4532 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4528.600 kb on + strand, within Psyr_3807 at 4528.600 kb on + strand, within Psyr_3807 at 4528.703 kb on + strand, within Psyr_3807 at 4528.703 kb on + strand, within Psyr_3807 at 4528.704 kb on - strand, within Psyr_3807 at 4528.704 kb on - strand, within Psyr_3807 at 4528.704 kb on - strand, within Psyr_3807 at 4528.729 kb on - strand, within Psyr_3807 at 4528.817 kb on - strand, within Psyr_3807 at 4528.993 kb on + strand, within Psyr_3807 at 4529.036 kb on - strand at 4529.081 kb on - strand at 4529.095 kb on + strand at 4529.148 kb on - strand at 4529.333 kb on + strand, within Psyr_3808 at 4529.509 kb on - strand, within Psyr_3808 at 4529.582 kb on + strand at 4529.611 kb on + strand at 4529.612 kb on - strand at 4529.645 kb on + strand at 4529.675 kb on - strand at 4529.678 kb on - strand at 4529.767 kb on + strand at 4529.809 kb on + strand, within Psyr_3809 at 4529.809 kb on + strand, within Psyr_3809 at 4529.888 kb on - strand, within Psyr_3809 at 4529.888 kb on - strand, within Psyr_3809 at 4529.934 kb on - strand, within Psyr_3809 at 4530.084 kb on + strand, within Psyr_3809 at 4530.158 kb on + strand, within Psyr_3809 at 4530.211 kb on + strand, within Psyr_3809 at 4530.370 kb on + strand, within Psyr_3809 at 4530.371 kb on - strand, within Psyr_3809 at 4530.371 kb on - strand, within Psyr_3809 at 4530.602 kb on - strand, within Psyr_3809 at 4530.608 kb on - strand, within Psyr_3809 at 4530.807 kb on + strand, within Psyr_3809 at 4530.808 kb on - strand, within Psyr_3809 at 4530.857 kb on + strand, within Psyr_3809 at 4530.985 kb on - strand, within Psyr_3809 at 4531.012 kb on - strand, within Psyr_3809 at 4531.115 kb on + strand, within Psyr_3809 at 4531.116 kb on - strand, within Psyr_3809 at 4531.118 kb on - strand, within Psyr_3809 at 4531.132 kb on + strand, within Psyr_3809 at 4531.136 kb on + strand, within Psyr_3809 at 4531.194 kb on + strand, within Psyr_3809 at 4531.293 kb on - strand at 4531.348 kb on + strand at 4531.376 kb on - strand at 4531.413 kb on - strand at 4531.413 kb on - strand at 4531.413 kb on - strand at 4531.453 kb on + strand at 4531.453 kb on + strand at 4531.453 kb on + strand at 4531.454 kb on - strand at 4531.455 kb on + strand at 4531.456 kb on - strand at 4531.456 kb on - strand at 4531.457 kb on + strand at 4531.457 kb on + strand at 4531.458 kb on - strand at 4531.552 kb on - strand at 4531.583 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Harmalol 10 mM remove 4,528,600 + Psyr_3807 0.57 -0.6 4,528,600 + Psyr_3807 0.57 -1.3 4,528,703 + Psyr_3807 0.66 -1.1 4,528,703 + Psyr_3807 0.66 -0.2 4,528,704 - Psyr_3807 0.66 -0.3 4,528,704 - Psyr_3807 0.66 +1.2 4,528,704 - Psyr_3807 0.66 -0.9 4,528,729 - Psyr_3807 0.68 +0.3 4,528,817 - Psyr_3807 0.75 -1.9 4,528,993 + Psyr_3807 0.90 +0.0 4,529,036 - +0.7 4,529,081 - -2.5 4,529,095 + -0.3 4,529,148 - -3.0 4,529,333 + Psyr_3808 0.46 -1.9 4,529,509 - Psyr_3808 0.82 +0.9 4,529,582 + -1.0 4,529,611 + -3.5 4,529,612 - +0.6 4,529,645 + -1.3 4,529,675 - -3.5 4,529,678 - +1.3 4,529,767 + -1.8 4,529,809 + Psyr_3809 0.12 -1.7 4,529,809 + Psyr_3809 0.12 -2.5 4,529,888 - Psyr_3809 0.16 -1.1 4,529,888 - Psyr_3809 0.16 -1.6 4,529,934 - Psyr_3809 0.19 -1.1 4,530,084 + Psyr_3809 0.27 -0.5 4,530,158 + Psyr_3809 0.31 -0.4 4,530,211 + Psyr_3809 0.34 -2.9 4,530,370 + Psyr_3809 0.42 +0.5 4,530,371 - Psyr_3809 0.42 -2.1 4,530,371 - Psyr_3809 0.42 +0.9 4,530,602 - Psyr_3809 0.55 -2.8 4,530,608 - Psyr_3809 0.55 -0.5 4,530,807 + Psyr_3809 0.66 -2.6 4,530,808 - Psyr_3809 0.66 +2.3 4,530,857 + Psyr_3809 0.69 -3.3 4,530,985 - Psyr_3809 0.75 -0.6 4,531,012 - Psyr_3809 0.77 -1.3 4,531,115 + Psyr_3809 0.82 +0.5 4,531,116 - Psyr_3809 0.82 -1.9 4,531,118 - Psyr_3809 0.83 -2.1 4,531,132 + Psyr_3809 0.83 -0.3 4,531,136 + Psyr_3809 0.84 +0.4 4,531,194 + Psyr_3809 0.87 -0.9 4,531,293 - -2.3 4,531,348 + -1.4 4,531,376 - +0.3 4,531,413 - -2.1 4,531,413 - -0.0 4,531,413 - -0.5 4,531,453 + -0.8 4,531,453 + +1.3 4,531,453 + +0.1 4,531,454 - -1.1 4,531,455 + +0.1 4,531,456 - +0.7 4,531,456 - -0.8 4,531,457 + -0.1 4,531,457 + -1.2 4,531,458 - -2.6 4,531,552 - -1.2 4,531,583 + +0.1
Or see this region's nucleotide sequence