Experiment: KB with Harmalol 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3398 and Psyr_3399 overlap by 11 nucleotides Psyr_3399 and Psyr_3400 are separated by 13 nucleotides
Psyr_3398: Psyr_3398 - Alanine racemase, N-terminal, at 4,055,262 to 4,056,398
_3398
Psyr_3399: Psyr_3399 - Endoribonuclease L-PSP, at 4,056,388 to 4,056,786
_3399
Psyr_3400: Psyr_3400 - FAD dependent oxidoreductase, at 4,056,800 to 4,057,984
_3400
Position (kb)
4056
4057 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 4055.508 kb on + strand, within Psyr_3398 at 4055.509 kb on - strand, within Psyr_3398 at 4055.509 kb on - strand, within Psyr_3398 at 4055.509 kb on - strand, within Psyr_3398 at 4055.509 kb on - strand, within Psyr_3398 at 4055.509 kb on - strand, within Psyr_3398 at 4055.597 kb on - strand, within Psyr_3398 at 4055.787 kb on + strand, within Psyr_3398 at 4055.787 kb on + strand, within Psyr_3398 at 4055.788 kb on - strand, within Psyr_3398 at 4055.788 kb on - strand, within Psyr_3398 at 4055.970 kb on + strand, within Psyr_3398 at 4055.970 kb on + strand, within Psyr_3398 at 4055.970 kb on + strand, within Psyr_3398 at 4055.970 kb on + strand, within Psyr_3398 at 4055.970 kb on + strand, within Psyr_3398 at 4055.970 kb on + strand, within Psyr_3398 at 4055.970 kb on + strand, within Psyr_3398 at 4055.970 kb on + strand, within Psyr_3398 at 4055.970 kb on + strand, within Psyr_3398 at 4055.970 kb on + strand, within Psyr_3398 at 4055.970 kb on + strand, within Psyr_3398 at 4055.970 kb on + strand, within Psyr_3398 at 4055.971 kb on - strand, within Psyr_3398 at 4055.971 kb on - strand, within Psyr_3398 at 4055.971 kb on - strand, within Psyr_3398 at 4055.971 kb on - strand, within Psyr_3398 at 4055.971 kb on - strand, within Psyr_3398 at 4055.971 kb on - strand, within Psyr_3398 at 4055.971 kb on - strand, within Psyr_3398 at 4055.971 kb on - strand, within Psyr_3398 at 4055.971 kb on - strand, within Psyr_3398 at 4055.971 kb on - strand, within Psyr_3398 at 4055.971 kb on - strand, within Psyr_3398 at 4055.971 kb on - strand, within Psyr_3398 at 4055.971 kb on - strand, within Psyr_3398 at 4056.339 kb on + strand at 4056.372 kb on - strand at 4056.628 kb on + strand, within Psyr_3399 at 4056.628 kb on + strand, within Psyr_3399 at 4056.629 kb on - strand, within Psyr_3399 at 4056.629 kb on - strand, within Psyr_3399 at 4056.629 kb on - strand, within Psyr_3399 at 4056.629 kb on - strand, within Psyr_3399 at 4056.771 kb on + strand at 4056.923 kb on + strand, within Psyr_3400 at 4056.924 kb on - strand, within Psyr_3400 at 4057.112 kb on + strand, within Psyr_3400 at 4057.113 kb on - strand, within Psyr_3400 at 4057.218 kb on - strand, within Psyr_3400 at 4057.374 kb on + strand, within Psyr_3400 at 4057.374 kb on + strand, within Psyr_3400 at 4057.374 kb on + strand, within Psyr_3400 at 4057.375 kb on - strand, within Psyr_3400 at 4057.375 kb on - strand, within Psyr_3400 at 4057.375 kb on - strand, within Psyr_3400 at 4057.375 kb on - strand, within Psyr_3400 at 4057.375 kb on - strand, within Psyr_3400 at 4057.375 kb on - strand, within Psyr_3400 at 4057.553 kb on + strand, within Psyr_3400 at 4057.553 kb on + strand, within Psyr_3400 at 4057.553 kb on + strand, within Psyr_3400 at 4057.553 kb on + strand, within Psyr_3400 at 4057.553 kb on + strand, within Psyr_3400 at 4057.554 kb on - strand, within Psyr_3400 at 4057.554 kb on - strand, within Psyr_3400 at 4057.606 kb on + strand, within Psyr_3400 at 4057.635 kb on - strand, within Psyr_3400 at 4057.635 kb on - strand, within Psyr_3400
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Harmalol 10 mM remove 4,055,508 + Psyr_3398 0.22 +0.6 4,055,509 - Psyr_3398 0.22 -0.4 4,055,509 - Psyr_3398 0.22 -1.0 4,055,509 - Psyr_3398 0.22 +1.5 4,055,509 - Psyr_3398 0.22 +0.5 4,055,509 - Psyr_3398 0.22 -1.1 4,055,597 - Psyr_3398 0.29 -0.0 4,055,787 + Psyr_3398 0.46 -0.6 4,055,787 + Psyr_3398 0.46 +1.1 4,055,788 - Psyr_3398 0.46 -0.3 4,055,788 - Psyr_3398 0.46 +0.1 4,055,970 + Psyr_3398 0.62 +0.1 4,055,970 + Psyr_3398 0.62 -1.2 4,055,970 + Psyr_3398 0.62 -0.6 4,055,970 + Psyr_3398 0.62 +1.4 4,055,970 + Psyr_3398 0.62 +1.3 4,055,970 + Psyr_3398 0.62 +0.5 4,055,970 + Psyr_3398 0.62 -0.6 4,055,970 + Psyr_3398 0.62 -1.1 4,055,970 + Psyr_3398 0.62 -0.1 4,055,970 + Psyr_3398 0.62 +0.3 4,055,970 + Psyr_3398 0.62 +0.1 4,055,970 + Psyr_3398 0.62 +0.5 4,055,971 - Psyr_3398 0.62 +0.5 4,055,971 - Psyr_3398 0.62 -1.0 4,055,971 - Psyr_3398 0.62 +1.9 4,055,971 - Psyr_3398 0.62 +0.9 4,055,971 - Psyr_3398 0.62 +1.0 4,055,971 - Psyr_3398 0.62 +0.4 4,055,971 - Psyr_3398 0.62 +0.0 4,055,971 - Psyr_3398 0.62 -3.0 4,055,971 - Psyr_3398 0.62 -0.4 4,055,971 - Psyr_3398 0.62 -0.7 4,055,971 - Psyr_3398 0.62 -0.6 4,055,971 - Psyr_3398 0.62 -0.9 4,055,971 - Psyr_3398 0.62 +0.7 4,056,339 + -1.0 4,056,372 - +0.1 4,056,628 + Psyr_3399 0.60 -0.0 4,056,628 + Psyr_3399 0.60 +0.5 4,056,629 - Psyr_3399 0.60 +0.7 4,056,629 - Psyr_3399 0.60 +0.4 4,056,629 - Psyr_3399 0.60 -0.2 4,056,629 - Psyr_3399 0.60 +0.5 4,056,771 + +0.0 4,056,923 + Psyr_3400 0.10 +0.0 4,056,924 - Psyr_3400 0.10 -2.0 4,057,112 + Psyr_3400 0.26 -1.3 4,057,113 - Psyr_3400 0.26 -1.1 4,057,218 - Psyr_3400 0.35 +2.6 4,057,374 + Psyr_3400 0.48 -0.1 4,057,374 + Psyr_3400 0.48 +4.2 4,057,374 + Psyr_3400 0.48 +0.4 4,057,375 - Psyr_3400 0.49 +0.8 4,057,375 - Psyr_3400 0.49 +2.1 4,057,375 - Psyr_3400 0.49 -0.4 4,057,375 - Psyr_3400 0.49 -0.6 4,057,375 - Psyr_3400 0.49 -1.2 4,057,375 - Psyr_3400 0.49 -0.0 4,057,553 + Psyr_3400 0.64 +0.1 4,057,553 + Psyr_3400 0.64 +1.3 4,057,553 + Psyr_3400 0.64 -0.1 4,057,553 + Psyr_3400 0.64 -0.1 4,057,553 + Psyr_3400 0.64 -2.0 4,057,554 - Psyr_3400 0.64 +0.8 4,057,554 - Psyr_3400 0.64 +1.9 4,057,606 + Psyr_3400 0.68 +0.2 4,057,635 - Psyr_3400 0.70 -0.5 4,057,635 - Psyr_3400 0.70 +0.3
Or see this region's nucleotide sequence