Experiment: KB with Harmalol 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3353 and Psyr_3354 are separated by 61 nucleotides Psyr_3354 and Psyr_3355 are separated by 12 nucleotides
Psyr_3353: Psyr_3353 - Catalase, at 4,010,342 to 4,011,874
_3353
Psyr_3354: Psyr_3354 - Ankyrin, at 4,011,936 to 4,012,505
_3354
Psyr_3355: Psyr_3355 - PAS, at 4,012,518 to 4,014,458
_3355
Position (kb)
4011
4012
4013 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4010.945 kb on + strand, within Psyr_3353 at 4010.945 kb on + strand, within Psyr_3353 at 4010.946 kb on - strand, within Psyr_3353 at 4010.997 kb on + strand, within Psyr_3353 at 4010.997 kb on + strand, within Psyr_3353 at 4010.997 kb on + strand, within Psyr_3353 at 4010.998 kb on - strand, within Psyr_3353 at 4010.998 kb on - strand, within Psyr_3353 at 4010.998 kb on - strand, within Psyr_3353 at 4010.998 kb on - strand, within Psyr_3353 at 4011.006 kb on - strand, within Psyr_3353 at 4011.038 kb on + strand, within Psyr_3353 at 4011.038 kb on + strand, within Psyr_3353 at 4011.039 kb on - strand, within Psyr_3353 at 4011.039 kb on - strand, within Psyr_3353 at 4011.039 kb on - strand, within Psyr_3353 at 4011.119 kb on + strand, within Psyr_3353 at 4011.119 kb on + strand, within Psyr_3353 at 4011.120 kb on - strand, within Psyr_3353 at 4011.120 kb on - strand at 4011.185 kb on + strand, within Psyr_3353 at 4011.185 kb on + strand, within Psyr_3353 at 4011.186 kb on - strand at 4011.186 kb on - strand, within Psyr_3353 at 4011.186 kb on - strand, within Psyr_3353 at 4011.414 kb on - strand, within Psyr_3353 at 4011.431 kb on + strand, within Psyr_3353 at 4011.431 kb on + strand, within Psyr_3353 at 4011.545 kb on + strand, within Psyr_3353 at 4011.545 kb on + strand, within Psyr_3353 at 4011.546 kb on - strand, within Psyr_3353 at 4011.546 kb on - strand, within Psyr_3353 at 4011.774 kb on - strand at 4011.779 kb on + strand at 4011.779 kb on + strand at 4011.779 kb on + strand at 4011.780 kb on - strand at 4011.780 kb on - strand at 4011.780 kb on - strand at 4011.780 kb on - strand at 4011.797 kb on + strand at 4011.797 kb on + strand at 4011.951 kb on + strand at 4012.125 kb on + strand, within Psyr_3354 at 4012.155 kb on + strand, within Psyr_3354 at 4012.155 kb on + strand, within Psyr_3354 at 4012.155 kb on + strand, within Psyr_3354 at 4012.155 kb on + strand, within Psyr_3354 at 4012.156 kb on - strand, within Psyr_3354 at 4012.156 kb on - strand, within Psyr_3354 at 4012.205 kb on - strand, within Psyr_3354 at 4012.205 kb on - strand, within Psyr_3354 at 4012.205 kb on - strand, within Psyr_3354 at 4012.368 kb on + strand, within Psyr_3354 at 4012.368 kb on + strand, within Psyr_3354 at 4012.368 kb on + strand, within Psyr_3354 at 4012.369 kb on - strand, within Psyr_3354 at 4012.369 kb on - strand, within Psyr_3354 at 4012.543 kb on + strand at 4012.813 kb on + strand, within Psyr_3355 at 4012.814 kb on - strand, within Psyr_3355 at 4012.814 kb on - strand, within Psyr_3355 at 4012.814 kb on - strand, within Psyr_3355 at 4012.814 kb on - strand, within Psyr_3355 at 4012.951 kb on + strand, within Psyr_3355 at 4012.952 kb on - strand, within Psyr_3355 at 4012.966 kb on + strand, within Psyr_3355 at 4012.966 kb on + strand, within Psyr_3355 at 4013.002 kb on + strand, within Psyr_3355 at 4013.003 kb on - strand, within Psyr_3355 at 4013.003 kb on - strand, within Psyr_3355 at 4013.042 kb on - strand, within Psyr_3355 at 4013.167 kb on + strand, within Psyr_3355 at 4013.167 kb on + strand, within Psyr_3355 at 4013.168 kb on - strand, within Psyr_3355
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Harmalol 10 mM remove 4,010,945 + Psyr_3353 0.39 +0.0 4,010,945 + Psyr_3353 0.39 +0.8 4,010,946 - Psyr_3353 0.39 -0.2 4,010,997 + Psyr_3353 0.43 +1.7 4,010,997 + Psyr_3353 0.43 -0.5 4,010,997 + Psyr_3353 0.43 -0.5 4,010,998 - Psyr_3353 0.43 -0.8 4,010,998 - Psyr_3353 0.43 -1.2 4,010,998 - Psyr_3353 0.43 +1.7 4,010,998 - Psyr_3353 0.43 -0.2 4,011,006 - Psyr_3353 0.43 -1.0 4,011,038 + Psyr_3353 0.45 +1.5 4,011,038 + Psyr_3353 0.45 -0.3 4,011,039 - Psyr_3353 0.45 -1.0 4,011,039 - Psyr_3353 0.45 -1.8 4,011,039 - Psyr_3353 0.45 +0.9 4,011,119 + Psyr_3353 0.51 -0.4 4,011,119 + Psyr_3353 0.51 -0.8 4,011,120 - Psyr_3353 0.51 -0.1 4,011,120 - +0.9 4,011,185 + Psyr_3353 0.55 -0.4 4,011,185 + Psyr_3353 0.55 -0.3 4,011,186 - +1.2 4,011,186 - Psyr_3353 0.55 -0.1 4,011,186 - Psyr_3353 0.55 -3.8 4,011,414 - Psyr_3353 0.70 -0.4 4,011,431 + Psyr_3353 0.71 +0.5 4,011,431 + Psyr_3353 0.71 +0.5 4,011,545 + Psyr_3353 0.78 -0.2 4,011,545 + Psyr_3353 0.78 +0.0 4,011,546 - Psyr_3353 0.79 +0.9 4,011,546 - Psyr_3353 0.79 +0.2 4,011,774 - -1.7 4,011,779 + -0.7 4,011,779 + +1.0 4,011,779 + -2.7 4,011,780 - -1.2 4,011,780 - -2.2 4,011,780 - +0.5 4,011,780 - -2.2 4,011,797 + -0.3 4,011,797 + +0.4 4,011,951 + -0.9 4,012,125 + Psyr_3354 0.33 -0.4 4,012,155 + Psyr_3354 0.38 +0.1 4,012,155 + Psyr_3354 0.38 +0.0 4,012,155 + Psyr_3354 0.38 +0.5 4,012,155 + Psyr_3354 0.38 +0.6 4,012,156 - Psyr_3354 0.39 +1.5 4,012,156 - Psyr_3354 0.39 +1.0 4,012,205 - Psyr_3354 0.47 +2.1 4,012,205 - Psyr_3354 0.47 +1.4 4,012,205 - Psyr_3354 0.47 -0.3 4,012,368 + Psyr_3354 0.76 -0.1 4,012,368 + Psyr_3354 0.76 -0.2 4,012,368 + Psyr_3354 0.76 +1.0 4,012,369 - Psyr_3354 0.76 +1.0 4,012,369 - Psyr_3354 0.76 -0.2 4,012,543 + +1.4 4,012,813 + Psyr_3355 0.15 -0.6 4,012,814 - Psyr_3355 0.15 +0.0 4,012,814 - Psyr_3355 0.15 -0.2 4,012,814 - Psyr_3355 0.15 -1.0 4,012,814 - Psyr_3355 0.15 -0.7 4,012,951 + Psyr_3355 0.22 -1.8 4,012,952 - Psyr_3355 0.22 -0.3 4,012,966 + Psyr_3355 0.23 -1.1 4,012,966 + Psyr_3355 0.23 +1.6 4,013,002 + Psyr_3355 0.25 +0.7 4,013,003 - Psyr_3355 0.25 -0.6 4,013,003 - Psyr_3355 0.25 +0.9 4,013,042 - Psyr_3355 0.27 -1.0 4,013,167 + Psyr_3355 0.33 -0.8 4,013,167 + Psyr_3355 0.33 -0.7 4,013,168 - Psyr_3355 0.33 -0.5
Or see this region's nucleotide sequence