Experiment: KB with Harmalol 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2947 and Psyr_2948 are separated by 46 nucleotides Psyr_2948 and Psyr_2949 are separated by 85 nucleotides Psyr_2949 and Psyr_2950 are separated by 198 nucleotides
Psyr_2947: Psyr_2947 - hypothetical protein, at 3,536,743 to 3,537,165
_2947
Psyr_2948: Psyr_2948 - Nitroreductase, at 3,537,212 to 3,537,856
_2948
Psyr_2949: Psyr_2949 - conserved hypothetical protein, at 3,537,942 to 3,538,526
_2949
Psyr_2950: Psyr_2950 - putative hydrolase signal peptide protein, at 3,538,725 to 3,539,672
_2950
Position (kb)
3537
3538
3539 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3536.970 kb on + strand, within Psyr_2947 at 3536.984 kb on - strand, within Psyr_2947 at 3536.984 kb on - strand, within Psyr_2947 at 3536.986 kb on - strand, within Psyr_2947 at 3537.011 kb on + strand, within Psyr_2947 at 3537.011 kb on + strand, within Psyr_2947 at 3537.012 kb on - strand, within Psyr_2947 at 3537.012 kb on - strand, within Psyr_2947 at 3537.012 kb on - strand, within Psyr_2947 at 3537.133 kb on + strand at 3537.133 kb on + strand at 3537.134 kb on - strand at 3537.134 kb on - strand at 3537.134 kb on - strand at 3537.247 kb on + strand at 3537.247 kb on + strand at 3537.247 kb on + strand at 3537.248 kb on - strand at 3537.248 kb on - strand at 3537.248 kb on - strand at 3537.248 kb on - strand at 3537.248 kb on - strand at 3537.290 kb on + strand, within Psyr_2948 at 3537.396 kb on + strand, within Psyr_2948 at 3537.475 kb on + strand, within Psyr_2948 at 3537.475 kb on + strand, within Psyr_2948 at 3537.475 kb on + strand, within Psyr_2948 at 3537.475 kb on + strand, within Psyr_2948 at 3537.476 kb on - strand, within Psyr_2948 at 3537.476 kb on - strand, within Psyr_2948 at 3537.563 kb on + strand, within Psyr_2948 at 3537.563 kb on + strand, within Psyr_2948 at 3537.564 kb on - strand, within Psyr_2948 at 3537.564 kb on - strand, within Psyr_2948 at 3537.564 kb on - strand, within Psyr_2948 at 3537.593 kb on - strand, within Psyr_2948 at 3537.596 kb on + strand, within Psyr_2948 at 3537.596 kb on + strand, within Psyr_2948 at 3537.596 kb on + strand, within Psyr_2948 at 3537.596 kb on + strand, within Psyr_2948 at 3537.597 kb on - strand, within Psyr_2948 at 3537.597 kb on - strand, within Psyr_2948 at 3537.597 kb on - strand, within Psyr_2948 at 3537.597 kb on - strand, within Psyr_2948 at 3537.597 kb on - strand, within Psyr_2948 at 3537.597 kb on - strand, within Psyr_2948 at 3537.676 kb on + strand, within Psyr_2948 at 3537.850 kb on + strand at 3537.854 kb on + strand at 3537.854 kb on + strand at 3538.100 kb on + strand, within Psyr_2949 at 3538.100 kb on + strand, within Psyr_2949 at 3538.100 kb on + strand, within Psyr_2949 at 3538.101 kb on - strand, within Psyr_2949 at 3538.156 kb on - strand, within Psyr_2949 at 3538.174 kb on + strand, within Psyr_2949 at 3538.174 kb on + strand, within Psyr_2949 at 3538.175 kb on - strand, within Psyr_2949 at 3538.175 kb on - strand, within Psyr_2949 at 3538.206 kb on + strand, within Psyr_2949 at 3538.388 kb on + strand, within Psyr_2949 at 3538.388 kb on + strand, within Psyr_2949 at 3538.388 kb on + strand, within Psyr_2949 at 3538.388 kb on + strand, within Psyr_2949 at 3538.389 kb on - strand, within Psyr_2949 at 3538.389 kb on - strand, within Psyr_2949 at 3538.389 kb on - strand, within Psyr_2949 at 3538.464 kb on + strand, within Psyr_2949 at 3538.464 kb on + strand, within Psyr_2949 at 3538.465 kb on - strand, within Psyr_2949 at 3538.535 kb on + strand at 3538.535 kb on + strand at 3538.535 kb on + strand at 3538.535 kb on + strand at 3538.536 kb on - strand at 3538.536 kb on - strand at 3538.536 kb on - strand at 3538.536 kb on - strand at 3538.706 kb on + strand at 3538.707 kb on - strand at 3538.707 kb on - strand at 3538.731 kb on + strand at 3538.738 kb on + strand at 3538.739 kb on - strand at 3538.814 kb on + strand at 3538.815 kb on - strand at 3538.815 kb on - strand at 3538.827 kb on - strand, within Psyr_2950 at 3538.868 kb on + strand, within Psyr_2950 at 3538.944 kb on + strand, within Psyr_2950 at 3538.945 kb on - strand, within Psyr_2950 at 3538.977 kb on + strand, within Psyr_2950 at 3538.978 kb on - strand, within Psyr_2950 at 3538.986 kb on + strand, within Psyr_2950 at 3538.986 kb on + strand, within Psyr_2950 at 3538.986 kb on + strand, within Psyr_2950 at 3538.986 kb on + strand, within Psyr_2950 at 3538.986 kb on + strand, within Psyr_2950 at 3538.986 kb on + strand, within Psyr_2950 at 3538.986 kb on + strand, within Psyr_2950 at 3538.986 kb on + strand, within Psyr_2950 at 3538.986 kb on + strand, within Psyr_2950 at 3538.986 kb on + strand, within Psyr_2950 at 3538.986 kb on + strand, within Psyr_2950 at 3538.986 kb on + strand, within Psyr_2950 at 3538.986 kb on + strand, within Psyr_2950 at 3538.986 kb on + strand, within Psyr_2950 at 3538.987 kb on - strand, within Psyr_2950 at 3538.987 kb on - strand, within Psyr_2950 at 3538.987 kb on - strand, within Psyr_2950 at 3538.987 kb on - strand, within Psyr_2950 at 3538.987 kb on - strand, within Psyr_2950 at 3538.987 kb on - strand, within Psyr_2950 at 3538.987 kb on - strand, within Psyr_2950 at 3538.987 kb on - strand, within Psyr_2950 at 3538.987 kb on - strand, within Psyr_2950 at 3538.987 kb on - strand, within Psyr_2950 at 3538.987 kb on - strand, within Psyr_2950 at 3538.987 kb on - strand, within Psyr_2950 at 3538.987 kb on - strand, within Psyr_2950 at 3538.987 kb on - strand, within Psyr_2950 at 3538.987 kb on - strand, within Psyr_2950 at 3538.987 kb on - strand, within Psyr_2950 at 3539.145 kb on + strand, within Psyr_2950 at 3539.146 kb on - strand, within Psyr_2950 at 3539.212 kb on - strand, within Psyr_2950 at 3539.212 kb on - strand, within Psyr_2950 at 3539.292 kb on + strand, within Psyr_2950 at 3539.292 kb on + strand, within Psyr_2950 at 3539.292 kb on + strand, within Psyr_2950 at 3539.293 kb on - strand, within Psyr_2950 at 3539.293 kb on - strand, within Psyr_2950 at 3539.426 kb on + strand, within Psyr_2950 at 3539.427 kb on - strand, within Psyr_2950 at 3539.427 kb on - strand, within Psyr_2950 at 3539.520 kb on + strand, within Psyr_2950
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Harmalol 10 mM remove 3,536,970 + Psyr_2947 0.54 +0.4 3,536,984 - Psyr_2947 0.57 -0.6 3,536,984 - Psyr_2947 0.57 +0.2 3,536,986 - Psyr_2947 0.57 -0.7 3,537,011 + Psyr_2947 0.63 +0.0 3,537,011 + Psyr_2947 0.63 -1.0 3,537,012 - Psyr_2947 0.64 -0.6 3,537,012 - Psyr_2947 0.64 -0.7 3,537,012 - Psyr_2947 0.64 +0.3 3,537,133 + +1.3 3,537,133 + -1.3 3,537,134 - +0.0 3,537,134 - -0.4 3,537,134 - +1.7 3,537,247 + +0.3 3,537,247 + +0.4 3,537,247 + +0.3 3,537,248 - +1.1 3,537,248 - +1.0 3,537,248 - +2.3 3,537,248 - -0.1 3,537,248 - -0.0 3,537,290 + Psyr_2948 0.12 +1.5 3,537,396 + Psyr_2948 0.29 -1.7 3,537,475 + Psyr_2948 0.41 +1.1 3,537,475 + Psyr_2948 0.41 -0.2 3,537,475 + Psyr_2948 0.41 -0.9 3,537,475 + Psyr_2948 0.41 -0.1 3,537,476 - Psyr_2948 0.41 -0.8 3,537,476 - Psyr_2948 0.41 +0.7 3,537,563 + Psyr_2948 0.54 -1.0 3,537,563 + Psyr_2948 0.54 +1.3 3,537,564 - Psyr_2948 0.55 -0.3 3,537,564 - Psyr_2948 0.55 +1.0 3,537,564 - Psyr_2948 0.55 +0.2 3,537,593 - Psyr_2948 0.59 +1.7 3,537,596 + Psyr_2948 0.60 -1.6 3,537,596 + Psyr_2948 0.60 +2.0 3,537,596 + Psyr_2948 0.60 -0.6 3,537,596 + Psyr_2948 0.60 +0.7 3,537,597 - Psyr_2948 0.60 -0.1 3,537,597 - Psyr_2948 0.60 -0.0 3,537,597 - Psyr_2948 0.60 +0.7 3,537,597 - Psyr_2948 0.60 +0.4 3,537,597 - Psyr_2948 0.60 +0.5 3,537,597 - Psyr_2948 0.60 +0.4 3,537,676 + Psyr_2948 0.72 +0.1 3,537,850 + -0.4 3,537,854 + -0.3 3,537,854 + +0.2 3,538,100 + Psyr_2949 0.27 -0.7 3,538,100 + Psyr_2949 0.27 -1.2 3,538,100 + Psyr_2949 0.27 +1.0 3,538,101 - Psyr_2949 0.27 +1.3 3,538,156 - Psyr_2949 0.37 +0.7 3,538,174 + Psyr_2949 0.40 +1.1 3,538,174 + Psyr_2949 0.40 +0.8 3,538,175 - Psyr_2949 0.40 +0.7 3,538,175 - Psyr_2949 0.40 -0.2 3,538,206 + Psyr_2949 0.45 -1.3 3,538,388 + Psyr_2949 0.76 +1.0 3,538,388 + Psyr_2949 0.76 +0.9 3,538,388 + Psyr_2949 0.76 +0.2 3,538,388 + Psyr_2949 0.76 +0.3 3,538,389 - Psyr_2949 0.76 +1.1 3,538,389 - Psyr_2949 0.76 +0.3 3,538,389 - Psyr_2949 0.76 +1.0 3,538,464 + Psyr_2949 0.89 -0.6 3,538,464 + Psyr_2949 0.89 -0.9 3,538,465 - Psyr_2949 0.89 +0.1 3,538,535 + +0.3 3,538,535 + +0.7 3,538,535 + -2.3 3,538,535 + +0.7 3,538,536 - -1.9 3,538,536 - +0.7 3,538,536 - +1.3 3,538,536 - +0.2 3,538,706 + +0.3 3,538,707 - +0.1 3,538,707 - -0.2 3,538,731 + -0.6 3,538,738 + +0.3 3,538,739 - -0.5 3,538,814 + +0.7 3,538,815 - -1.5 3,538,815 - -0.7 3,538,827 - Psyr_2950 0.11 -0.1 3,538,868 + Psyr_2950 0.15 +0.0 3,538,944 + Psyr_2950 0.23 -0.2 3,538,945 - Psyr_2950 0.23 +1.3 3,538,977 + Psyr_2950 0.27 -0.7 3,538,978 - Psyr_2950 0.27 -1.7 3,538,986 + Psyr_2950 0.28 +0.7 3,538,986 + Psyr_2950 0.28 -0.9 3,538,986 + Psyr_2950 0.28 +3.3 3,538,986 + Psyr_2950 0.28 -0.9 3,538,986 + Psyr_2950 0.28 -1.8 3,538,986 + Psyr_2950 0.28 +1.0 3,538,986 + Psyr_2950 0.28 +0.5 3,538,986 + Psyr_2950 0.28 -2.3 3,538,986 + Psyr_2950 0.28 -0.2 3,538,986 + Psyr_2950 0.28 +1.1 3,538,986 + Psyr_2950 0.28 -2.3 3,538,986 + Psyr_2950 0.28 +0.3 3,538,986 + Psyr_2950 0.28 +0.4 3,538,986 + Psyr_2950 0.28 +0.3 3,538,987 - Psyr_2950 0.28 +0.1 3,538,987 - Psyr_2950 0.28 -0.3 3,538,987 - Psyr_2950 0.28 -0.3 3,538,987 - Psyr_2950 0.28 +1.4 3,538,987 - Psyr_2950 0.28 -1.8 3,538,987 - Psyr_2950 0.28 +0.3 3,538,987 - Psyr_2950 0.28 +0.4 3,538,987 - Psyr_2950 0.28 -1.2 3,538,987 - Psyr_2950 0.28 +1.1 3,538,987 - Psyr_2950 0.28 -1.9 3,538,987 - Psyr_2950 0.28 +0.4 3,538,987 - Psyr_2950 0.28 -0.9 3,538,987 - Psyr_2950 0.28 +0.8 3,538,987 - Psyr_2950 0.28 +0.2 3,538,987 - Psyr_2950 0.28 +1.3 3,538,987 - Psyr_2950 0.28 -2.8 3,539,145 + Psyr_2950 0.44 -0.5 3,539,146 - Psyr_2950 0.44 -0.4 3,539,212 - Psyr_2950 0.51 -0.5 3,539,212 - Psyr_2950 0.51 +0.1 3,539,292 + Psyr_2950 0.60 -2.9 3,539,292 + Psyr_2950 0.60 +0.7 3,539,292 + Psyr_2950 0.60 +0.4 3,539,293 - Psyr_2950 0.60 +1.0 3,539,293 - Psyr_2950 0.60 -0.2 3,539,426 + Psyr_2950 0.74 -1.0 3,539,427 - Psyr_2950 0.74 -0.7 3,539,427 - Psyr_2950 0.74 -0.3 3,539,520 + Psyr_2950 0.84 -1.3
Or see this region's nucleotide sequence